Mercurial > repos > john-mccallum > pcr_markers
diff design_primers.xml @ 5:b321e0517be3 draft
Uploaded
author | ben-warren |
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date | Thu, 22 May 2014 20:30:19 -0400 |
parents | a0689dc29b7f |
children | f201e8c6e004 |
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--- a/design_primers.xml Thu Oct 18 19:47:07 2012 -0400 +++ b/design_primers.xml Thu May 22 20:30:19 2014 -0400 @@ -1,14 +1,25 @@ <?xml version="1.0"?> <tool id="Genetic_Marker_Design_2" name="Design primers to features"> <description>Design PCR Primers to Features</description> - <command interpreter="python">design_primers.py $inputfastaFile $inputSNPfile $inputTargetfile $min_size $max_size > $primer_outputfile </command> + <command interpreter="python -W ignore"> + #if 'Yes' in $uMelt.value + design_primers.py -i $inputfastaFile -g $inputSNPfile -T $inputTargetfile -p $min_size -P $max_size -u > $primer_outputfile + #else + design_primers.py -i $inputfastaFile -g $inputSNPfile -T $inputTargetfile -p $min_size -P $max_size > $primer_outputfile + #end if + </command> + <inputs> <param format="fasta" name="inputfastaFile" type="data" label="Multifasta Source file" /> <param format="gff3" name="inputSNPfile" type="data" label="annotation file(Gff3)" /> <param format="txt" name="inputTargetfile" type="data" optional="false" label="Target file" help="IN FORMAT Sequence id:source:type:start e.g. 1174806:gsMapper:SNP:292" ></param> <param name="min_size" size="20" type="text" value="75" label="Minimum Product Size Range" /> <param name="max_size" size="20" type="text" value="100" label="Maximum Product Size Range" /> - </inputs> + <param name="uMelt" type="select" label="uMelt prediction for HRM"> + <option value="No" selected= "true">No</option> + <option value="Yes">Yes</option> + </param> + </inputs> <outputs> <data format="tabular" name="primer_outputfile" /> </outputs> @@ -18,6 +29,7 @@ **TIP** This tool designs primer pairs to flank features +Optionally it can predict reference and variant Tm using the uMelt web service provided by University of Utah It takes