Mercurial > repos > john-mccallum > pcr_markers
view GVF_Features_Extracter.xml @ 2:ea2117a7b363 draft
Uploaded CAPS marker design workflow for 454 Data
author | john-mccallum |
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date | Sun, 09 Sep 2012 23:16:56 -0400 |
parents | 21053f7f9ed1 |
children |
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<?xml version="1.0"?> <tool id="GVF Features Extracter_1" name="GVF Feature ID Extracter"> <description>Extract unique feature IDs from a GVF/GFF3 file </description> <command >awk '!/^#/ {print $1":"$2":"$3":"$4}' $inputgff3File | sort | uniq > $column_outputfile</command> <inputs> <param format="gff3" name="inputgff3File" type="data" label="GVF/Gff3 File"/> </inputs> <outputs> <data format="tabular" name="column_outputfile" /> </outputs> <help> .. class:: infomark **TIP** This tool extracts a unique feature identifier from GVF/GFF3 format, primarily to enable downstream analysis of SNPs or marker design ---- **Example** *input GFF3* :: ##gff-version 3 1119927 gsMapper SNP 434 434 . . . ID=1119927:gsMapper:SNP:434;Reference_seq=G;Variant_seq=A;Total_reads=10;Variant_freq=100;Enzyme=AluI;Phase=variant 1120709 gsMapper SNP 361 361 . . . ID=1120709:gsMapper:SNP:361;Reference_seq=T;Variant_seq=C;Total_reads=22;Variant_freq=68;Enzyme=TaqI;Phase=variant 1120709 gsMapper SNP 704 704 . . . ID=1120709:gsMapper:SNP:704;Reference_seq=A;Variant_seq=G;Total_reads=20;Variant_freq=90;Enzyme=RsaI;Phase=variant *output tabular text* :: 1119927:gsMapper:SNP:434 1120709:gsMapper:SNP:361 1120709:gsMapper:SNP:704 ----------------------------------------------------- *If you use this tool please cite:* A Toolkit For Bulk PCR-Based Marker Design From Next-Generation Sequence Data: Application For Development Of A Framework Linkage Map In Bulb Onion (*Allium cepa* L.) (2012) Samantha Baldwin, Roopashree Revanna, Susan Thomson, Meeghan Pither-Joyce, Kathryn Wright, Ross Crowhurst, Mark Fiers, Leshi Chen, Richard MacKnight, John A. McCallum </help> </tool>