Mercurial > repos > jpruab > jpr_picard
diff picard_FastqToSam.xml @ 4:f4d018471628 draft default tip
Uploaded
author | jpruab |
---|---|
date | Tue, 13 Aug 2013 12:09:14 -0400 |
parents | |
children |
line wrap: on
line diff
--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/picard_FastqToSam.xml Tue Aug 13 12:09:14 2013 -0400 @@ -0,0 +1,145 @@ +<tool id="picard_FastqToSam" name="FASTQ to BAM" version="1.56.0"> + <description>creates an unaligned BAM file</description> + <requirements><requirement type="package" version="1.56.0">picard</requirement></requirements> + <!-- Dan Blankenberg --> + <command>java -XX:DefaultMaxRAMFraction=1 -XX:+UseParallelGC + -jar "\$JAVA_JAR_PATH/FastqToSam.jar" + FASTQ="${input_fastq1}" + #if str( $input_fastq2) != "None": + FASTQ2="${input_fastq2}" + #end if + QUALITY_FORMAT="${ dict( fastqsanger='Standard', fastqcssanger='Standard', fastqillumina='Illumina', fastqsolexa='Solexa' )[ $input_fastq1.ext ] }" ##Solexa, Illumina, Standard + OUTPUT="${output_bam}" + READ_GROUP_NAME="${read_group_name}" + SAMPLE_NAME="${sample_name}" + #if $param_type.param_type_selector == "advanced": + #if str( $param_type.library_name ) != "": + LIBRARY_NAME="${param_type.library_name}" + #end if + #if str( $param_type.platform_unit ) != "": + PLATFORM_UNIT="${param_type.platform_unit}" + #end if + #if str( $param_type.platform ) != "": + PLATFORM="${param_type.platform}" + #end if + #if str( $param_type.sequencing_center ) != "": + SEQUENCING_CENTER="${param_type.sequencing_center}" + #end if + #if str( $param_type.predicted_insert_size ) != "": + PREDICTED_INSERT_SIZE="${param_type.predicted_insert_size}" + #end if + #if str( $param_type.description.value ) != "": + DESCRIPTION="${param_type.description}" + #end if + #if str( $param_type.run_date ) != "": + RUN_DATE="${param_type.run_date}" + #end if + #if str( $param_type.min_q ) != "": + MIN_Q="${param_type.min_q}" + #end if + #if str( $param_type.max_q ) != "": + MAX_Q="${param_type.max_q}" + #end if + SORT_ORDER="${param_type.sort_order}" + #else: + SORT_ORDER=coordinate ##unsorted, queryname, coordinate; always use coordinate + #end if + 2>&1 + || echo "Error running Picard FastqToSAM" >&2 + </command> + <inputs> + <param name="input_fastq1" type="data" format="fastqsanger,fastqillumina,fastqsolexa,fastqcssanger" label="FASTQ file" /> <!-- confirm that fastqcssanger also works --> + <param name="input_fastq2" type="data" format="fastqsanger,fastqillumina,fastqsolexa,fastqcssanger" optional="True" label="Second FASTQ of paired end data" help="Only needed when using paired end data." > + <options options_filter_attribute="ext" from_parameter="tool.app.datatypes_registry.datatypes_by_extension" transform_lines="obj.keys()"> + <column name="name" index="0"/> + <column name="value" index="0"/> + <filter type="param_value" ref="input_fastq1" ref_attribute="ext" column="0"/> + </options> + </param> + <param name="read_group_name" type="text" value="A" label="Read Group Name" /> + <param name="sample_name" type="text" value="unknown sample" label="Sample Name" /> + <conditional name="param_type"> + <param name="param_type_selector" type="select" label="Basic or Advanced options"> + <option value="basic" selected="True">Basic</option> + <option value="advanced">Advanced</option> + </param> + <when value="basic"> + <!-- Do nothing here --> + </when> + <when value="advanced"> + <param name="library_name" type="text" value="" label="Library Name" /> + <param name="platform_unit" type="text" value="" label="Platform Unit" /> + <param name="platform" type="text" value="" label="Platform" /> + <param name="sequencing_center" type="text" value="" label="Sequencing Center" /> + <param name="predicted_insert_size" type="integer" value="" optional="True" label="Predicted Insert Size" /> + <param name="description" type="text" value="" label="Description" /> + <param name="run_date" type="text" value="" label="Run Date" /> + <param name="min_q" type="integer" optional="True" value="0" label="Min Q" /> + <param name="max_q" type="integer" optional="True" value="93" label="Max Q" /> + <param name="sort_order" type="select" label="Sort order"> + <option value="coordinate" selected="True">coordinate</option> + <option value="queryname">queryname</option> + <option value="unsorted">unsorted</option> + </param> + </when> + </conditional> + </inputs> + <outputs> + <data format="bam" name="output_bam" /> + </outputs> + <tests> + <test> + <param name="input_fastq1" value="bwa_wrapper_in2.fastqsanger" ftype="fastqsanger" /> + <param name="input_fastq2" /> + <param name="read_group_name" value="A" /> + <param name="sample_name" value="unknown sample" /> + <param name="param_type_selector" value="basic" /> + <output name="output_bam" file="picard_fastq_to_sam_out1.bam" ftype="bam"/> + </test> + <test> + <param name="input_fastq1" value="bwa_wrapper_in2.fastqsanger" ftype="fastqsanger" /> + <param name="input_fastq2" value="bwa_wrapper_in3.fastqsanger" ftype="fastqsanger" /> + <param name="read_group_name" value="A" /> + <param name="sample_name" value="unknown sample" /> + <param name="param_type_selector" value="basic" /> + <output name="output_bam" file="picard_fastq_to_sam_out2.bam" ftype="bam"/> + </test> + </tests> + <help> +**What it does** + +Picard: FastqToSam converts FASTQ files to unaligned BAM files. + +------ + +Please cite the website "http://picard.sourceforge.net". + +------ + + +**Input formats** + +FastqToSam accepts FASTQ input files. If using paired-end data, you should select two FASTQ files. + +------ + +**Outputs** + +The output is in BAM format, see http://samtools.sourceforge.net for more details. + +------- + +**FastqToSam settings** + +This is list of FastqToSam options:: + + READ_GROUP_NAME=String Read group name Default value: A. This option can be set to 'null' to clear the default value. + SAMPLE_NAME=String Sample name to insert into the read group header Required. + LIBRARY_NAME=String The library name to place into the LB attribute in the read group header Default value: null. + PLATFORM_UNIT=String The platform unit (often run_barcode.lane) to insert into the read group header Default value: null. + PLATFORM=String The platform type (e.g. illumina, solid) to insert into the read group header Default value: null. + SEQUENCING_CENTER=String The sequencing center from which the data originated Default value: null. + PREDICTED_INSERT_SIZE=Integer Predicted median insert size, to insert into the read group header Default value: null. + DESCRIPTION=String Inserted into the read group header Default value: null. + </help> +</tool>