Mercurial > repos > konradpaszkiewicz > interproscan
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| author | peterjc |
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| date | Wed, 05 Jun 2013 13:40:56 -0400 |
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1 #Created 07/01/2011 - Konrad Paszkiewicz, Exeter Sequencing Service, University of Exeter, UK |
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2 Revisions 2013 by Peter Cock, The James Hutton Institute, UK |
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4 The attached is a crude wrapper script for Interproscan. Typically this is useful when one wants to produce an annotation which is not based on sequence |
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5 similarity. E.g after a denovo transcriptome assembly, each transcript could be translated and run through this tool. |
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7 Prerequisites: |
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9 1. A working installation of Interproscan on your Galaxy server/cluster. |
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11 Limitations: |
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13 Currently it is setup to work with PFAM only due to the heavy computational demands Interproscan makes. |
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15 Input formats: |
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17 The standard interproscan input is either genomic or protein sequences. In the case of genomic sequences Interproscan will of run an ORF |
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18 prediction tool. However this tends to lose the ORF information (e.g. start/end co-ordinates) from the header. As such the requirement here is to input ORF |
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19 sequences (e.g. from EMBOSS getorf) and to then replace any spaces in the FASTA header with underscores. This workaround generally preserves the relevant |
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20 positional information. |
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