Mercurial > repos > konradpaszkiewicz > preppereadsforveletfasta
diff preppereads.xml @ 0:4d237a31970b default tip
Migrated tool version 1.0.0 from old tool shed archive to new tool shed repository
author | konradpaszkiewicz |
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date | Tue, 07 Jun 2011 17:42:21 -0400 |
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--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/preppereads.xml Tue Jun 07 17:42:21 2011 -0400 @@ -0,0 +1,32 @@ +<tool id="preppereadsforvelet" name="Prepare paired-end reads for use with Velvet (FASTQ)" version="1.0.0"> + <description>Prepare paired-end reads for use with Velvet</description> + <command interpreter="python"> + preppereads.py + '$__app__.config.new_file_path' + '$input1' + '$input2' + '$outpe' + '$outsingletons'; exit 0 + </command> + <inputs> + <param name="input1" type="data" format="illuminafastq" label="Read 1 of paired-end dataset"/> + <param name="input2" type="data" format="illuminafastq" label="Read 2 of paired-end dataset"/> + </inputs> + <outputs> + <data format="illuminafastq" name="outpe" label="Merged paired-end reads ready for velvet"/> + <data format="illuminafastq" name="outsingletons" label="Singleton reads reads for velvet"/> + + </outputs> + <requirements> + </requirements> + <help> +**Paired-end reads preparation for velvet** + +Velvet requires paired-end reads to be in the same file in an interleaved FASTQ format. This format specifies that read 1 should be followed immediately by read 2. + +This script is useful to ensure reads are in the correct order prior to passing the data to Velvet. + +Singleton reads can also be produced if quality trimming or other filtering criteria have removed the read's mate. These can also be passed to Velvet using a second short read channel. + + </help> +</tool>