Mercurial > repos > konradpaszkiewicz > preppereadsforveletfasta
view preppereads.py @ 0:4d237a31970b default tip
Migrated tool version 1.0.0 from old tool shed archive to new tool shed repository
author | konradpaszkiewicz |
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date | Tue, 07 Jun 2011 17:42:21 -0400 |
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#!/usr/bin/env python """ Classes encapsulating decypher tool. James E Johnson - University of Minnesota """ import pkg_resources; import logging, os, string, sys, tempfile, glob, shutil, types, urllib import shlex, subprocess from optparse import OptionParser, OptionGroup from stat import * log = logging.getLogger( __name__ ) assert sys.version_info[:2] >= ( 2, 4 ) def stop_err( msg ): sys.stderr.write( "%s\n" % msg ) sys.exit() def __main__(): #Parse Command Line s = 'preppereads.py: argv = %s\n' % (sys.argv) # print >> sys.stderr, s # so will appear as blurb for file argcnt = len(sys.argv) working_dir = sys.argv[1] input1 = sys.argv[2] input2 = sys.argv[3] outpe = sys.argv[4] outsingletons = sys.argv[5] cmdline = '/users/galaxy/galaxyscripts/prepare_pe_reads_for_velvet.sh %s %s %s %s %s %s > /dev/null' % (input1, '1.fastq', input2, '2.fastq', 'single.fq', 'pe.fq') #print >> sys.stderr, cmdline # so will appear as blurb for file try: proc = subprocess.Popen( args=cmdline, shell=True, stderr=subprocess.PIPE ) returncode = proc.wait() # get stderr, allowing for case where it's very large stderr = '' buffsize = 1048576 try: while True: stderr += proc.stderr.read( buffsize ) if not stderr or len( stderr ) % buffsize != 0: break except OverflowError: pass if returncode != 0: raise Exception, stderr except Exception, e: stop_err( 'Error running preppereads.sh ' + str( e ) ) out = open(outpe,'w') #outpe_path = os.path.join(working_dir,'') for line in open('pe.fq'): out.write( "%s" % (line) ) out.close() out = open(outsingletons,'w') #singletons_path = os.path.join(working_dir,'') for line in open('single.fq'): out.write( "%s" % (line) ) out.close() if __name__ == "__main__": __main__()