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1 /******************************************************************************
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2 ** @source gb1
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3 **
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4 ** Calculate strand bias of bacterial genome using B1 index
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5 **
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6 ** @author Copyright (C) 2012 Hidetoshi Itaya
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7 ** @version 1.0.3
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8 ** @modified 2012/1/20 Hidetoshi Itaya Created!
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9 ** @modified 2013/6/16 Revision 1
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10 ** @modified 2015/2/7 Refactor
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11 ** @@
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12 **
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13 ** This program is free software; you can redistribute it and/or
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14 ** modify it under the terms of the GNU General Public License
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15 ** as published by the Free Software Foundation; either version 2
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16 ** of the License, or (at your option) any later version.
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17 **
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18 ** This program is distributed in the hope that it will be useful,
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19 ** but WITHOUT ANY WARRANTY; without even the implied warranty of
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20 ** MERCHANTABILITY or FITNESS FOR A PARTICULAR PURPOSE. See the
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21 ** GNU General Public License for more details.
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22 **
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23 ** You should have received a copy of the GNU General Public License
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24 ** along with this program; if not, write to the Free Software
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25 ** Foundation, Inc., 59 Temple Place - Suite 330, Boston, MA 02111-1307, USA.
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26 ******************************************************************************/
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27
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28 #include "emboss.h"
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29 #include "glibs.h"
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30
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31
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32
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33
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34 /* @prog gb1 ******************************************************************
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35 **
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36 ** Calculate strand bias of bacterial genome using B1 index
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37 **
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38 ******************************************************************************/
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39
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40 int main(int argc, char *argv[])
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41 {
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42 embInitPV("gb1", argc, argv, "GEMBASSY", "1.0.3");
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43
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44 AjPSeqall seqall;
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45 AjPSeq seq;
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46 AjPStr inseq = NULL;
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47
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48 AjBool accid = ajFalse;
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49 AjPStr restid = NULL;
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50 AjPStr seqid = NULL;
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51
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52 AjPStr base = NULL;
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53 AjPStr url = NULL;
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54
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55 AjPStr method = NULL;
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56
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57 AjPFile outf = NULL;
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58
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59 AjPFile tmpfile = NULL;
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60 AjPStr tmpname = NULL;
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61
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62 AjPFilebuff tmp = NULL;
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63 AjPStr line = NULL;
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64
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65 seqall = ajAcdGetSeqall("sequence");
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66 method = ajAcdGetSelectSingle("method");
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67 accid = ajAcdGetBoolean("accid");
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68 outf = ajAcdGetOutfile("outfile");
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69
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70 base = ajStrNewC("rest.g-language.org");
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71
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72 gAssignUniqueName(&tmpname);
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73
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74 while(ajSeqallNext(seqall, &seq))
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75 {
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76 inseq = NULL;
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77
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78 if(!accid)
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79 {
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80 if(gFormatGenbank(seq, &inseq))
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81 {
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82 tmpfile = ajFileNewOutNameS(tmpname);
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83 if(!tmpfile)
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84 {
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85 ajDie("Output file (%S) open error\n", tmpname);
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86 }
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87 ajFmtPrintF(tmpfile, "%S", inseq);
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88 ajFileClose(&tmpfile);
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89 ajFmtPrintS(&url, "http://%S/upload/upl.pl", base);
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90 gFilePostSS(url, tmpname, &restid);
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91 ajStrDel(&url);
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92 ajSysFileUnlinkS(tmpname);
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93 }
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94 else
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95 {
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96 ajWarn("Sequence does not have features\n"
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97 "Proceeding with sequence accession ID\n");
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98 accid = ajTrue;
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99 }
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100 }
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101
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102 ajStrAssignS(&seqid, ajSeqGetAccS(seq));
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103
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104 if(ajStrGetLen(seqid) == 0)
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105 {
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106 ajStrAssignS(&seqid, ajSeqGetNameS(seq));
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107 }
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108
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109 if(ajStrGetLen(seqid) == 0)
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110 {
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111 ajWarn("No valid header information\n");
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112 }
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113
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114 if(accid)
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115 {
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116 ajStrAssignS(&restid, seqid);
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117 if(ajStrGetLen(seqid) == 0)
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118 {
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119 ajDie("Cannot proceed without header with -accid\n");
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120 }
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121
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122 if(!gValID(seqid))
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123 {
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124 ajDie("Invalid accession ID:%S, exiting\n", seqid);
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125 }
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126 }
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127
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128 url = ajStrNew();
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129
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130 ajFmtPrintS(&url, "http://%S/%S/B1/method=%S", base, restid, method);
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131
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132 if(!gFilebuffURLS(url, &tmp))
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133 {
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134 ajDie("Failed to download result from:\n%S\n", url);
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135 }
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136
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137 ajBuffreadLine(tmp, &line);
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138
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139 ajStrRemoveSetC(&line, "\n");
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140
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141 ajFmtPrintF(outf, "Sequence: %S B1: %S\n", seqid, line);
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142
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143 ajStrDel(&url);
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144 ajStrDel(&restid);
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145 ajStrDel(&seqid);
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146 ajStrDel(&inseq);
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147 }
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148
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149 ajFileClose(&outf);
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150
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151 ajSeqallDel(&seqall);
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152 ajSeqDel(&seq);
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153 ajStrDel(&base);
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154
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155 ajStrDel(&method);
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156
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157 embExit();
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158
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159 return 0;
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160 }
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