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date | Fri, 26 Jun 2015 05:21:44 -0400 |
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1 <!--START OF HEADER - DON'T ALTER --> | |
2 | |
3 <HTML> | |
4 <HEAD> | |
5 <TITLE> EMBOSS: gaaui </TITLE> | |
6 </HEAD> | |
7 <BODY BGCOLOR="#FFFFFF" text="#000000"> | |
8 | |
9 | |
10 | |
11 <table align=center border=0 cellspacing=0 cellpadding=0> | |
12 <tr><td valign=top> | |
13 <A HREF="/" ONMOUSEOVER="self.status='Go to the EMBOSS home page';return true"><img border=0 src="http://soap.g-language.org/gembassy/emboss_explorer/manual/emboss_icon.jpg" alt="" width=150 height=48></a> | |
14 </td> | |
15 <td align=left valign=middle> | |
16 <b><font size="+6"> | |
17 gaaui | |
18 </font></b> | |
19 </td></tr> | |
20 </table> | |
21 <br> | |
22 <p> | |
23 | |
24 | |
25 <!--END OF HEADER--> | |
26 | |
27 | |
28 | |
29 | |
30 | |
31 | |
32 <H2> Function </H2> | |
33 Calculates various indece of amino acid usage | |
34 <!-- | |
35 DON'T WRITE ANYTHING HERE. | |
36 IT IS DONE FOR YOU. | |
37 --> | |
38 | |
39 | |
40 | |
41 | |
42 <H2>Description</H2> | |
43 <p> | |
44 gaaui calculates amino acid usage indices for proteins (excluding<br /> | |
45 formylmethionine). Calculated indices are as follows,<br /> | |
46 Laa: Length in amino acids<br /> | |
47 ndaa: Number of different amino acids<br /> | |
48 Haau: Entropy of amino acid usage<br /> | |
49 mmw: Mean molecular weight<br /> | |
50 gravy: Mean hydropathic indices of each amino acid<br /> | |
51 aroma: Relative frequency of aromatic amino acids<br /> | |
52 <br /> | |
53 G-language SOAP service is provided by the<br /> | |
54 Institute for Advanced Biosciences, Keio University.<br /> | |
55 The original web service is located at the following URL:<br /> | |
56 <br /> | |
57 http://www.g-language.org/wiki/soap<br /> | |
58 <br /> | |
59 WSDL(RPC/Encoded) file is located at:<br /> | |
60 <br /> | |
61 http://soap.g-language.org/g-language.wsdl<br /> | |
62 <br /> | |
63 Documentation on G-language Genome Analysis Environment methods are<br /> | |
64 provided at the Document Center<br /> | |
65 <br /> | |
66 http://ws.g-language.org/gdoc/<br /> | |
67 <br /> | |
68 | |
69 </p> | |
70 | |
71 <H2>Usage</H2> | |
72 | |
73 Here is a sample session with gaaui | |
74 | |
75 <table width="90%"><tr><td bgcolor="#CCFFFF"><pre> | |
76 | |
77 % gaaui refseqn:NC_000913 | |
78 Calculates various indece of amino acid usage | |
79 AAINDEX entry output file [nc_000913.gaaui]: | |
80 | |
81 </pre></td></tr></table> | |
82 | |
83 Go to the <a href="#input">input files</a> for this example<br> | |
84 Go to the <a href="#output">output files</a> for this example<br><br> | |
85 | |
86 <h2>Command line arguments</h2> | |
87 | |
88 <table border cellspacing=0 cellpadding=3 bgcolor="#ccccff"> | |
89 <tr bgcolor="#FFFFCC"> | |
90 <th align="left">Qualifier</th> | |
91 <th align="left">Type</th> | |
92 <th align="left">Description</th> | |
93 <th align="left">Allowed values</th> | |
94 <th align="left">Default</th> | |
95 </tr> | |
96 | |
97 <tr bgcolor="#FFFFCC"> | |
98 <th align="left" colspan=5>Standard (Mandatory) qualifiers</th> | |
99 </tr> | |
100 | |
101 <tr bgcolor="#FFFFCC"> | |
102 <td>[-sequence]<br>(Parameter 1)</td> | |
103 <td>seqall</td> | |
104 <td>Nucleotide sequence(s) filename and optional format, or reference (input USA)</td> | |
105 <td>Readable sequence(s)</td> | |
106 <td><b>Required</b></td> | |
107 </tr> | |
108 | |
109 <tr bgcolor="#FFFFCC"> | |
110 <td>[-outfile]<br>(Parameter 2)</td> | |
111 <td>outfile</td> | |
112 <td>AAINDEX entry output file</td> | |
113 <td>Output file</td> | |
114 <td><i><*></i>.gaaui</td> | |
115 </tr> | |
116 | |
117 <tr bgcolor="#FFFFCC"> | |
118 <th align="left" colspan=5>Additional (Optional) qualifiers</th> | |
119 </tr> | |
120 | |
121 <tr> | |
122 <td colspan=5>(none)</td> | |
123 </tr> | |
124 | |
125 <tr bgcolor="#FFFFCC"> | |
126 <th align="left" colspan=5>Advanced (Unprompted) qualifiers</th> | |
127 </tr> | |
128 | |
129 <tr bgcolor="#FFFFCC"> | |
130 <td>-[no]accid</td> | |
131 <td>boolean</td> | |
132 <td>Include to use sequence accession ID as query</td> | |
133 <td>Boolean value Yes/No</td> | |
134 <td>Yes</td> | |
135 </tr> | |
136 | |
137 </table> | |
138 | |
139 | |
140 <h2 id="input">Input file format</h2> | |
141 | |
142 <p> | |
143 The database definitions for following commands are available at<br /> | |
144 http://soap.g-language.org/kbws/embossrc<br /> | |
145 <br /> | |
146 gaaui reads one or more nucleotide sequences.<br /> | |
147 <br /> | |
148 | |
149 </p> | |
150 | |
151 <h2 id="output">Output file format</h2> | |
152 | |
153 <p> | |
154 The output from gaaui is to a plain text file.<br /> | |
155 <br /> | |
156 File: nc_000913.gaaui<br /> | |
157 <br /> | |
158 <table width="90%"><tr><td bgcolor="#CCFFCC"> | |
159 Sequence: NC_000913<br /> | |
160 Laa,ndaa,Haau,mmw,gravy,aroma,gene<br /> | |
161 20,8,2.4842,117.48,+0.0150,0.0000,thrL<br /> | |
162 819,20,4.0887,126.65,+0.0328,0.0659,thrA<br /> | |
163 309,20,4.1228,126.35,+0.0181,0.0712,thrB<br /> | |
164 427,20,4.0806,128.00,-0.1014,0.0843,thrC<br /> | |
165 97,18,3.9165,133.54,-1.0268,0.0928,yaaX<br /> | |
166 257,19,4.0733,132.55,-0.4117,0.1089,yaaA<br /> | |
167 475,20,4.0413,126.46,+0.6781,0.1242,yaaJ<br /> | |
168 316,20,4.0395,128.99,-0.2165,0.0728,talB<br /> | |
169 <br /> | |
170 <font color=red>[Part of this file has been deleted for brevity]</font><br /> | |
171 <br /> | |
172 169,20,4.0001,124.90,+0.0231,0.0710,yjjX<br /> | |
173 214,20,3.9937,129.77,-0.3813,0.0374,ytjC<br /> | |
174 288,20,4.1421,132.58,-0.3628,0.1111,rob<br /> | |
175 156,20,4.0627,126.72,-0.0442,0.0705,creA<br /> | |
176 228,20,4.0471,131.94,-0.1408,0.0789,creB<br /> | |
177 473,20,4.0254,128.01,+0.0023,0.0677,creC<br /> | |
178 449,20,4.0871,128.66,+0.2082,0.0980,creD<br /> | |
179 237,20,4.0729,132.54,-0.4970,0.0675,arcA<br /> | |
180 45,15,3.5800,123.27,+0.7533,0.0222,yjjY<br /> | |
181 227,20,4.0283,128.63,-0.0031,0.0573,yjtD<br /> | |
182 </td></tr></table> | |
183 <br /> | |
184 <br /> | |
185 | |
186 </p> | |
187 | |
188 <h2>Data files</h2> | |
189 | |
190 <p> | |
191 None. | |
192 </p> | |
193 | |
194 <h2>Notes</h2> | |
195 | |
196 <p> | |
197 None. | |
198 </p> | |
199 | |
200 <h2>References</h2> | |
201 | |
202 <pre> | |
203 Lobry, JR. and Gautier, C. (1994) Hydrophobicity, expressivity and | |
204 aromaticity are the major trends of amino-acid usage in 999 Escherichia | |
205 coli chromosome-encoded genes, Nucleic Acids Res, 22:3174-3180.a | |
206 | |
207 Zavala A et al. (2002) Trends in codon and amino acid usage in Thermotoga | |
208 maritima J Mol Evol. 54(5):563-8. | |
209 | |
210 Arakawa, K., Mori, K., Ikeda, K., Matsuzaki, T., Konayashi, Y., and | |
211 Tomita, M. (2003) G-language Genome Analysis Environment: A Workbench | |
212 for Nucleotide Sequence Data Mining, Bioinformatics, 19, 305-306. | |
213 | |
214 Arakawa, K. and Tomita, M. (2006) G-language System as a Platform for | |
215 large-scale analysis of high-throughput omics data, J. Pest Sci., | |
216 31, 7. | |
217 | |
218 Arakawa, K., Kido, N., Oshita, K., Tomita, M. (2010) G-language Genome | |
219 Analysis Environment with REST and SOAP Web Service Interfaces, | |
220 Nucleic Acids Res., 38, W700-W705. | |
221 | |
222 </pre> | |
223 | |
224 <h2>Warnings</h2> | |
225 | |
226 <p> | |
227 None. | |
228 </p> | |
229 | |
230 <h2>Diagnostic Error Messages</h2> | |
231 | |
232 <p> | |
233 None. | |
234 </p> | |
235 | |
236 <h2>Exit status</h2> | |
237 | |
238 <p> | |
239 It always exits with a status of 0. | |
240 </p> | |
241 | |
242 <h2>Known bugs</h2> | |
243 | |
244 <p> | |
245 None. | |
246 </p> | |
247 | |
248 <h2>See also</h2> | |
249 | |
250 <table border cellpadding=4 bgcolor="#FFFFF0"><tr><th>Program name</th> | |
251 <th>Description</th></tr> | |
252 | |
253 <tr> | |
254 <td><a href="gaminoinfo.html">gaminoinfo</a></td> | |
255 <td>Prints out basic amino acid sequence statistics</td> | |
256 </tr><tr> | |
257 <td><a href="gcodoncompiler.html">gcodoncompiler</a></td> | |
258 <td>Calculate various kinds of amino acid and codon usage data</td> | |
259 </tr> | |
260 | |
261 </table> | |
262 | |
263 <h2>Author(s)</h2> | |
264 | |
265 <pre> | |
266 Hidetoshi Itaya (celery@g-language.org) | |
267 Institute for Advanced Biosciences, Keio University | |
268 252-0882 Japan | |
269 | |
270 Kazuharu Arakawa (gaou@sfc.keio.ac.jp) | |
271 Institute for Advanced Biosciences, Keio University | |
272 252-0882 Japan</pre> | |
273 | |
274 <h2>History</h2> | |
275 | |
276 2012 - Written by Hidetoshi Itaya | |
277 | |
278 <h2>Target users</h2> | |
279 | |
280 This program is intended to be used by everyone and everything, from | |
281 naive users to embedded scrips. | |
282 | |
283 <h2>Comments</h2> | |
284 | |
285 None. | |
286 |