Mercurial > repos > ktnyt > gembassy
diff GEMBASSY-1.0.3/doc/text/gaminoinfo.txt @ 0:8300eb051bea draft
Initial upload
author | ktnyt |
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date | Fri, 26 Jun 2015 05:19:29 -0400 |
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--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/GEMBASSY-1.0.3/doc/text/gaminoinfo.txt Fri Jun 26 05:19:29 2015 -0400 @@ -0,0 +1,219 @@ + gaminoinfo +Function + + Prints out basic amino acid sequence statistics + +Description + + gaminoinfo prints out basic compositional statistics of the given amino + acid sequence in a human readble manner. The calculated values are molecular + weight, number of residues, average residue weight, charge, isoelectric + point, number/mole/Dayhoffstat of each amino acid, and percentage of + Tiny (A+C+G+S+T), Small (A+B+C+D+G+N+P+S+T+V), Aliphatic (I+L+V), + Armoatic (F+H+W+Y), Non-polar (A+C+F+G+I+L+M+P+V+W+Y), + Polar (D+E+H+K+N+Q+R+S+T+Z), Charged (B+D+E+H+K+R+Z), Basic (H+K+R), and + Acidic (B+D+E+Z) reidues. + + + G-language SOAP service is provided by the + Institute for Advanced Biosciences, Keio University. + The original web service is located at the following URL: + + http://www.g-language.org/wiki/soap + + WSDL(RPC/Encoded) file is located at: + + http://soap.g-language.org/g-language.wsdl + + Documentation on G-language Genome Analysis Environment methods are + provided at the Document Center + + http://ws.g-language.org/gdoc/ + +Usage + +Here is a sample session with gaminoinfo + +% gaminoinfo tsw:hbb_human +Prints out basic amino acid sequence statistics +AAINDEX entry output file [hbb_human.gaminoinfo]: + + Go to the input files for this example + Go to the output files for this example + +Command line arguments + + Standard (Mandatory) qualifiers: + [-sequence] seqall Protein sequence(s) filename and optional + format, or reference (input USA) + [-outfile] outfile [*.gaminoinfo] AAINDEX entry output file + + Additional (Optional) qualifiers: (none) + Advanced (Unprompted) qualifiers: (none) + Associated qualifiers: + + "-sequence" associated qualifiers + -sbegin1 integer Start of each sequence to be used + -send1 integer End of each sequence to be used + -sreverse1 boolean Reverse (if DNA) + -sask1 boolean Ask for begin/end/reverse + -snucleotide1 boolean Sequence is nucleotide + -sprotein1 boolean Sequence is protein + -slower1 boolean Make lower case + -supper1 boolean Make upper case + -scircular1 boolean Sequence is circular + -sformat1 string Input sequence format + -iquery1 string Input query fields or ID list + -ioffset1 integer Input start position offset + -sdbname1 string Database name + -sid1 string Entryname + -ufo1 string UFO features + -fformat1 string Features format + -fopenfile1 string Features file name + + "-outfile" associated qualifiers + -odirectory2 string Output directory + + General qualifiers: + -auto boolean Turn off prompts + -stdout boolean Write first file to standard output + -filter boolean Read first file from standard input, write + first file to standard output + -options boolean Prompt for standard and additional values + -debug boolean Write debug output to program.dbg + -verbose boolean Report some/full command line options + -help boolean Report command line options and exit. More + information on associated and general + qualifiers can be found with -help -verbose + -warning boolean Report warnings + -error boolean Report errors + -fatal boolean Report fatal errors + -die boolean Report dying program messages + -version boolean Report version number and exit + +Input file format + + The database definitions for following commands are available at + http://soap.g-language.org/kbws/embossrc + + gaminoinfo reads one or more protein sequences. + +Output file format + + The output from gaminoinfo is to a plain text file. + + File: hbb_human.gaminoinfo + +Sequence: P68871 +AminoInfo of from 1 to 158 + +Molecular weight = 19309.27 Residues = 158 +Average Weight = 122.21 Charge = 3.5 +Isoelectric Point = 7.4065 +Residue Number Mole% DayhoffStat + + = 1 0.633 0.000 +> = 1 0.633 0.000 +A = Ala 16 10.127 1.178 +B = Asx 2 1.266 0.000 +C = Cys 2 1.266 0.436 +D = Asp 7 4.430 0.806 +E = Glu 8 5.063 0.844 +F = Phe 8 5.063 1.406 +G = Gly 13 8.228 0.980 +H = His 11 6.962 3.481 +K = Lys 11 6.962 1.055 +L = Leu 18 11.392 1.540 +M = Met 3 1.899 1.117 +N = Asn 7 4.430 1.030 +P = Pro 7 4.430 0.852 +Q = Gln 3 1.899 0.487 +R = Arg 3 1.899 0.387 +S = Ser 5 3.165 0.452 +T = Thr 7 4.430 0.726 +U = Sec 1 0.633 0.000 +V = Val 18 11.392 1.726 +W = Trp 2 1.266 0.974 +Y = Tyr 3 1.899 0.558 +_ = 1 0.633 0.000 + +Property Residues Number Mole% +Tiny (A+C+G+S+T) 43 27.215 +Small (A+B+C+D+G+N+P+S+T+V) 84 53.165 +Aliphatic (I+L+V) 36 22.785 +Aromatic (F+H+W+Y) 24 15.190 +Non-polar (A+C+F+G+I+L+M+P+V+W+Y) 90 56.962 +Polar (D+E+H+K+N+Q+R+S+T+Z) 62 39.241 +Charged (B+D+E+H+K+R+Z) 42 26.582 +Basic (H+K+R) 25 15.823 +Acidic (B+D+E+Z) 17 10.759 + + + +Data files + + None. + +Notes + + None. + +References + + Arakawa, K., Mori, K., Ikeda, K., Matsuzaki, T., Konayashi, Y., and + Tomita, M. (2003) G-language Genome Analysis Environment: A Workbench + for Nucleotide Sequence Data Mining, Bioinformatics, 19, 305-306. + + Arakawa, K. and Tomita, M. (2006) G-language System as a Platform for + large-scale analysis of high-throughput omics data, J. Pest Sci., + 31, 7. + + Arakawa, K., Kido, N., Oshita, K., Tomita, M. (2010) G-language Genome + Analysis Environment with REST and SOAP Web Service Interfaces, + Nucleic Acids Res., 38, W700-W705. + +Warnings + + None. + +Diagnostic Error Messages + + None. + +Exit status + + It always exits with a status of 0. + +Known bugs + + None. + +See also + + gaaui Calculates various indece of amino acid usage + gcodoncompiler Calculate various kinds of amino acid and codon usage data + +Author(s) + + Hidetoshi Itaya (celery@g-language.org) + Institute for Advanced Biosciences, Keio University + 252-0882 Japan + + Kazuharu Arakawa (gaou@sfc.keio.ac.jp) + Institute for Advanced Biosciences, Keio University + 252-0882 Japan + +History + + 2012 - Written by Hidetoshi Itaya + 2013 - Fixed by Hidetoshi Itaya + +Target users + + This program is intended to be used by everyone and everything, from + naive users to embedded scripts. + +Comments + + None. +