Mercurial > repos > ktnyt > gembassy
diff GEMBASSY-1.0.3/doc/text/gbaseentropy.txt @ 0:8300eb051bea draft
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author | ktnyt |
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date | Fri, 26 Jun 2015 05:19:29 -0400 |
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--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/GEMBASSY-1.0.3/doc/text/gbaseentropy.txt Fri Jun 26 05:19:29 2015 -0400 @@ -0,0 +1,243 @@ + gbaseentropy +Function + + Calculates and graphs the sequence conservation using Shanon uncertainty + +Description + + This function calculates and graphs the sequence conservation in regions + around the start/stop codons using Shanon uncertainty (entropy). Smaller + values resemble higher conservation where the minumum value is 0 and the + maximum value is 2. The entropy is typically the lowest around position 0 + (start/stop codon position). + + The entropy H at position i with distribution P(i) is calculated as follows: + H(P(i)) = -sum(P(i,j) * log(2,P(i,j))) + + G-language SOAP service is provided by the + Institute for Advanced Biosciences, Keio University. + The original web service is located at the following URL: + + http://www.g-language.org/wiki/soap + + WSDL(RPC/Encoded) file is located at: + + http://soap.g-language.org/g-language.wsdl + + Documentation on G-language Genome Analysis Environment methods are + provided at the Document Center + + http://ws.g-language.org/gdoc/ + +Usage + +Here is a sample session with gbaseentropy + +% gbaseentropy refseqn:NC_000913 +Calculates and graphs the sequence conservation using Shanon uncertainty +(entropy) +Program compseq output file (optional) [nc_000913.gbaseentropy]: + + Go to the input files for this example + Go to the output files for this example + + Example 2 + +% gbaseentropy refseqn:NC_000913 -plot -graph png +Calculates and graphs the sequence conservation using Shanon uncertainty +(entropy) +Created gbaseentropy.1.png + + Go to the input files for this example + Go to the output files for this example + +Command line arguments + +Calculates and graphs the sequence conservation using Shanon uncertainty +(entropy) +Version: EMBOSS:6.5.7.0 GEMBASSY:1.0.1 + + Standard (Mandatory) qualifiers (* if not always prompted): + [-sequence] seqall Nucleotide sequence(s) filename and optional + format, or reference (input USA) +* -graph xygraph [$EMBOSS_GRAPHICS value, or x11] Graph type + (ps, hpgl, hp7470, hp7580, meta, cps, x11, + tek, tekt, none, data, xterm, png, gif, svg) +* -outfile outfile [*.gbaseentropy] Program compseq output file + (optional) + + Additional (Optional) qualifiers: (none) + Advanced (Unprompted) qualifiers: + -position selection [start] Either 'start' (around start codon) + or 'end' (around stop codon) to create the + PWM + -patlen integer [3] Length of oligomer to count (Any integer + value) + -upstream integer [30] Length upstream of specified position + to create PWM (Any integer value) + -downstream integer [30] Length downstream of specified position + to create PWM (Any integer value) + -[no]accid boolean [Y] Include to use sequence accession ID as + query + -plot toggle [N] Include to plot result + + Associated qualifiers: + + "-sequence" associated qualifiers + -sbegin1 integer Start of each sequence to be used + -send1 integer End of each sequence to be used + -sreverse1 boolean Reverse (if DNA) + -sask1 boolean Ask for begin/end/reverse + -snucleotide1 boolean Sequence is nucleotide + -sprotein1 boolean Sequence is protein + -slower1 boolean Make lower case + -supper1 boolean Make upper case + -scircular1 boolean Sequence is circular + -sformat1 string Input sequence format + -iquery1 string Input query fields or ID list + -ioffset1 integer Input start position offset + -sdbname1 string Database name + -sid1 string Entryname + -ufo1 string UFO features + -fformat1 string Features format + -fopenfile1 string Features file name + + "-graph" associated qualifiers + -gprompt boolean Graph prompting + -gdesc string Graph description + -gtitle string Graph title + -gsubtitle string Graph subtitle + -gxtitle string Graph x axis title + -gytitle string Graph y axis title + -goutfile string Output file for non interactive displays + -gdirectory string Output directory + + "-outfile" associated qualifiers + -odirectory string Output directory + + General qualifiers: + -auto boolean Turn off prompts + -stdout boolean Write first file to standard output + -filter boolean Read first file from standard input, write + first file to standard output + -options boolean Prompt for standard and additional values + -debug boolean Write debug output to program.dbg + -verbose boolean Report some/full command line options + -help boolean Report command line options and exit. More + information on associated and general + qualifiers can be found with -help -verbose + -warning boolean Report warnings + -error boolean Report errors + -fatal boolean Report fatal errors + -die boolean Report dying program messages + -version boolean Report version number and exit + +Input file format + + The database definitions for following commands are available at + http://soap.g-language.org/kbws/embossrc + + gbaseentropy reads one or more nucleotide sequences. + +Output file format + + The output from gbaseentropy is to a plain text file or the EMBOSS + graphics device. + + File: nc_000913.gbaseentropy + +Sequence: NC_000913 +-30,1.98284 +-29,1.97873 +-28,1.97692 +-27,1.97595 +-26,1.97094 +-25,1.96777 +-24,1.96272 +-23,1.96288 +-22,1.95707 + + [Part of this file has been deleted for brevity] + +21,1.93528 +22,1.94470 +23,1.95204 +24,1.93139 +25,1.95640 +26,1.95711 +27,1.93785 +28,1.96060 +29,1.94316 +30,1.92581 + + +Data files + + None. + +Notes + + None. + +References + + Arakawa, K., Mori, K., Ikeda, K., Matsuzaki, T., Konayashi, Y., and + Tomita, M. (2003) G-language Genome Analysis Environment: A Workbench + for Nucleotide Sequence Data Mining, Bioinformatics, 19, 305-306. + + Arakawa, K. and Tomita, M. (2006) G-language System as a Platform for + large-scale analysis of high-throughput omics data, J. Pest Sci., + 31, 7. + + Arakawa, K., Kido, N., Oshita, K., Tomita, M. (2010) G-language Genome + Analysis Environment with REST and SOAP Web Service Interfaces, + Nucleic Acids Res., 38, W700-W705. + +Warnings + + None. + +Diagnostic Error Messages + + None. + +Exit status + + It always exits with a status of 0. + +Known bugs + + None. + +See also + + gbaseinformationcontent Calculates and graphs the sequence conservation + using information content + gbaserelativeentropy Calculates and graphs the sequence conservation + using Kullback-Leibler divergence (relative + entropy) + +Author(s) + + Hidetoshi Itaya (celery@g-language.org) + Institute for Advanced Biosciences, Keio University + 252-0882 Japan + + Kazuharu Arakawa (gaou@sfc.keio.ac.jp) + Institute for Advanced Biosciences, Keio University + 252-0882 Japan + +History + + 2012 - Written by Hidetoshi Itaya + 2013 - Fixed by Hidetoshi Itaya + +Target users + + This program is intended to be used by everyone and everything, from + naive users to embedded scripts. + +Comments + + None. +