--- /dev/null	Thu Jan 01 00:00:00 1970 +0000
+++ b/GEMBASSY-1.0.3/doc/text/genc.txt	Fri Jun 26 05:19:29 2015 -0400
@@ -0,0 +1,211 @@
+                                      genc
+Function
+
+   Calculate the effective number of codons (Nc)
+
+Description
+
+   genc calculates the effective number of codons (ENC|Nc). ENC is a measure
+   for species-independent codon usage bias. Some measures including CAI are
+   species-dependent as optimal codons differ. ENC assigns a gene a number
+   between 20 to 61 where 20 indicates that one codon is used for each amino
+   acid and 61 indicates that each codon is used equally throughout the
+   protein sequence.
+    
+   G-language SOAP service is provided by the
+   Institute for Advanced Biosciences, Keio University.
+   The original web service is located at the following URL:
+
+   http://www.g-language.org/wiki/soap
+
+   WSDL(RPC/Encoded) file is located at:
+
+   http://soap.g-language.org/g-language.wsdl
+
+   Documentation on G-language Genome Analysis Environment methods are
+   provided at the Document Center
+
+   http://ws.g-language.org/gdoc/
+
+Usage
+
+Here is a sample session with genc
+
+% genc refseqn:NC_000913
+Calculate the effective number of codons (Nc)
+Codon usage output file [nc_000913.genc]: 
+
+   Go to the input files for this example
+   Go to the output files for this example
+
+Command line arguments
+
+   Standard (Mandatory) qualifiers:
+  [-sequence]          seqall     Nucleotide sequence(s) filename and optional
+                                  format, or reference (input USA)
+  [-outfile]           outfile    [*.genc] Codon usage output file
+
+   Additional (Optional) qualifiers: (none)
+   Advanced (Unprompted) qualifiers:
+   -translate          boolean    [N] Include when translates using standard
+                                  codon table
+   -delkey             string     [[^ACDEFGHIKLMNPQRSTVWYacgtU]] Regular
+                                  expression to delete key (i.e. amino acids
+                                  and nucleotides) (Any string)
+   -[no]accid          boolean    [Y] Include to use sequence accession ID as
+                                  query
+
+   Associated qualifiers:
+
+   "-sequence" associated qualifiers
+   -sbegin1            integer    Start of each sequence to be used
+   -send1              integer    End of each sequence to be used
+   -sreverse1          boolean    Reverse (if DNA)
+   -sask1              boolean    Ask for begin/end/reverse
+   -snucleotide1       boolean    Sequence is nucleotide
+   -sprotein1          boolean    Sequence is protein
+   -slower1            boolean    Make lower case
+   -supper1            boolean    Make upper case
+   -scircular1         boolean    Sequence is circular
+   -sformat1           string     Input sequence format
+   -iquery1            string     Input query fields or ID list
+   -ioffset1           integer    Input start position offset
+   -sdbname1           string     Database name
+   -sid1               string     Entryname
+   -ufo1               string     UFO features
+   -fformat1           string     Features format
+   -fopenfile1         string     Features file name
+
+   "-outfile" associated qualifiers
+   -odirectory2        string     Output directory
+
+   General qualifiers:
+   -auto               boolean    Turn off prompts
+   -stdout             boolean    Write first file to standard output
+   -filter             boolean    Read first file from standard input, write
+                                  first file to standard output
+   -options            boolean    Prompt for standard and additional values
+   -debug              boolean    Write debug output to program.dbg
+   -verbose            boolean    Report some/full command line options
+   -help               boolean    Report command line options and exit. More
+                                  information on associated and general
+                                  qualifiers can be found with -help -verbose
+   -warning            boolean    Report warnings
+   -error              boolean    Report errors
+   -fatal              boolean    Report fatal errors
+   -die                boolean    Report dying program messages
+   -version            boolean    Report version number and exit
+
+Input file format
+
+   The database definitions for following commands are available at
+   http://soap.g-language.org/kbws/embossrc
+
+   genc reads one or more nucleotide sequences.
+
+Output file format
+
+   The output from genc is to a plain text file.
+
+   File: nc_000913.genc
+
+Sequence: NC_000913
+enc,gene
+,thrL
+48.41,thrA
+54.13,thrB
+46.18,thrC
+51.65,yaaX
+45.71,yaaA
+48.54,yaaJ
+36.83,talB
+
+   [Part of this file has been deleted for brevity]
+
+51.43,yjjX
+46.61,ytjC
+49.83,rob
+47.74,creA
+50.63,creB
+51.39,creC
+48.42,creD
+41.53,arcA
+61,yjjY
+53.63,yjtD
+
+
+Data files
+
+   None.
+
+Notes
+
+   None.
+
+References
+
+   Comeron JM, Aguade M. (1998) An evaluation of measures of synonymous codon
+      usage bias, J Mol Evol, 47(3):268-74.
+
+   Wright F. (1990) The 'effective number of codons' used in a gene, Gene,
+      87:23-29.
+
+   Arakawa, K., Mori, K., Ikeda, K., Matsuzaki, T., Konayashi, Y., and
+      Tomita, M. (2003) G-language Genome Analysis Environment: A Workbench
+      for Nucleotide Sequence Data Mining, Bioinformatics, 19, 305-306.
+
+   Arakawa, K. and Tomita, M. (2006) G-language System as a Platform for
+      large-scale analysis of high-throughput omics data, J. Pest Sci.,
+      31, 7.
+
+   Arakawa, K., Kido, N., Oshita, K., Tomita, M. (2010) G-language Genome
+      Analysis Environment with REST and SOAP Web Service Interfaces,
+      Nucleic Acids Res., 38, W700-W705.
+
+Warnings
+
+   None.
+
+Diagnostic Error Messages
+
+   None.
+
+Exit status
+
+   It always exits with a status of 0.
+
+Known bugs
+
+   None.
+
+See also
+
+   gew     Calculate a measure of synonymous codon usage evenness (Ew)
+   gfop    Calculate the frequency of optimal codons (Fop)
+   gscs    Calculates the scaled chi-square
+   gwvalue Calculate the 'relative adaptiveness of each codon' (W)
+
+Author(s)
+
+   Hidetoshi Itaya (celery@g-language.org)
+   Institute for Advanced Biosciences, Keio University
+   252-0882 Japan
+
+   Kazuharu Arakawa (gaou@sfc.keio.ac.jp)
+   Institute for Advanced Biosciences, Keio University
+   252-0882 Japan
+
+History
+
+   2012 - Written by Hidetoshi Itaya
+   2013 - Fixed by Hidetoshi Itaya
+
+Target users
+
+   This program is intended to be used by everyone and everything, from
+   naive users to embedded scripts.
+
+Comments
+
+   None.
+