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  <TITLE> EMBOSS: gbasezvalue </TITLE>
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<A HREF="/" ONMOUSEOVER="self.status='Go to the EMBOSS home page';return true"><img border=0 src="http://soap.g-language.org/gembassy/emboss_explorer/manual/emboss_icon.jpg" alt="" width=150 height=48></a>
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gbasezvalue
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<H2> Function </H2>
   Extracts conserved oligomers per position using Z-score
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<H2>Description</H2>
<p>
   This function calculates and extracts conserved oligomers per position using<br />
   Z-score, in regions around the start/stop codons. The oligomers are returned<br />
   in ranking order up to "-limit" number.<br />
    <br />
   G-language SOAP service is provided by the<br />
   Institute for Advanced Biosciences, Keio University.<br />
   The original web service is located at the following URL:<br />
<br />
   http://www.g-language.org/wiki/soap<br />
<br />
   WSDL(RPC/Encoded) file is located at:<br />
<br />
   http://soap.g-language.org/g-language.wsdl<br />
<br />
   Documentation on G-language Genome Analysis Environment methods are<br />
   provided at the Document Center<br />
<br />
   http://ws.g-language.org/gdoc/<br />
<br />

</p>

<H2>Usage</H2>

Here is a sample session with gbasezvalue

<table width="90%"><tr><td bgcolor="#CCFFFF"><pre>

% gbasezvalue refseqn:NC_000913
Extracts conserved oligomers per position using Z-score
Program compseq output file (optional) [nc_000913.gbasezvalue]: 

</pre></td></tr></table>

Go to the <a href="#input">input files</a> for this example<br>
Go to the <a href="#output">output files</a> for this example<br><br>



<h2>Command line arguments</h2>

<table border cellspacing=0 cellpadding=3 bgcolor="#ccccff">
<tr bgcolor="#FFFFCC">
<th align="left">Qualifier</th>
<th align="left">Type</th>
<th align="left">Description</th>
<th align="left">Allowed values</th>
<th align="left">Default</th>
</tr>

<tr bgcolor="#FFFFCC">
<th align="left" colspan=5>Standard (Mandatory) qualifiers</th>
</tr>

<tr bgcolor="#FFFFCC">
<td>[-sequence]<br>(Parameter 1)</td>
<td>seqall</td>
<td>Nucleotide sequence(s) filename and optional format, or reference (input USA)</td>
<td>Readable sequence(s)</td>
<td><b>Required</b></td>
</tr>

<tr bgcolor="#FFFFCC">
<td>[-outfile]<br>(Parameter 2)</td>
<td>outfile</td>
<td>Program compseq output file (optional)</td>
<td>Output file</td>
<td><i>&lt;*&gt;</i>.gbasezvalue</td>
</tr>

<tr bgcolor="#FFFFCC">
<th align="left" colspan=5>Additional (Optional) qualifiers</th>
</tr>

<tr>
<td colspan=5>(none)</td>
</tr>

<tr bgcolor="#FFFFCC">
<th align="left" colspan=5>Advanced (Unprompted) qualifiers</th>
</tr>

<tr bgcolor="#FFFFCC">
<td>-limit</td>
<td>integer</td>
<td>Rank threshold for showing the conserved oligomer</td>
<td>Any integer value</td>
<td>5</td>
</tr>

<tr bgcolor="#FFFFCC">
<td>-position</td>
<td>selection</td>
<td>Either 'start' (around start codon) or 'end' (around stop codon) to create the PWM</td>
<td>Choose from selection list of values</td>
<td>start</td>
</tr>

<tr bgcolor="#FFFFCC">
<td>-patlen</td>
<td>integer</td>
<td>Length of oligomer to count</td>
<td>Any integer value</td>
<td>3</td>
</tr>

<tr bgcolor="#FFFFCC">
<td>-upstream</td>
<td>integer</td>
<td>Length upstream of specified position to create PWM</td>
<td>Any integer value</td>
<td>30</td>
</tr>

<tr bgcolor="#FFFFCC">
<td>-downstream</td>
<td>integer</td>
<td>Length downstream of specified position to create PWM</td>
<td>Any integer value</td>
<td>30</td>
</tr>

<tr bgcolor="#FFFFCC">
<td>-[no]accid</td>
<td>boolean</td>
<td>Include to use sequence accession ID as query</td>
<td>Boolean value Yes/No</td>
<td>Yes</td>
</tr>

</table>


<h2 id="input">Input file format</h2>

<p>
   The database definitions for following commands are available at<br />
   http://soap.g-language.org/kbws/embossrc<br />
<br />
   gbasezvalue reads one or more nucleotide sequences.<br />
<br />

</p>

<h2 id="output">Output file format</h3>

<p>
   The output from gbasezvalue is to a plain text file.<br />
<br />
   File: nc_000913.gbasezvalue<br />
<br />
<table width="90%"><tr><td bgcolor="#CCFFCC">
Sequence: NC_000913<br />
position:-30,1,taa,-0.76525<br />
2,aga,-0.79101<br />
3,tta,-1.14174<br />
4,cta,-1.18831<br />
5,aat,-1.86652<br />
position:-29,1,cta,-0.18368<br />
2,aat,-0.71851<br />
3,gac,-1.26182<br />
4,taa,-1.39455<br />
<br />
<font color=red>[Part of this file has been deleted for brevity]</font><br />
<br />
position:29,1,gct,1.66288<br />
2,act,1.26637<br />
3,tat,0.66721<br />
4,cct,-0.43158<br />
5,tgt,-0.59254<br />
position:30,1,ctg,3.12072<br />
2,att,0.04193<br />
3,ctc,-0.12416<br />
4,cta,-0.38461<br />
5,tta,-0.76413<br />
</td></tr></table>

</p>

<h2>Data files</h2>

<p>
None.
</p>

<h2>Notes</h2>

<p>
None.
</p>

<h2>References</h2>

<pre>
   Arakawa, K., Mori, K., Ikeda, K., Matsuzaki, T., Konayashi, Y., and
      Tomita, M. (2003) G-language Genome Analysis Environment: A Workbench
      for Nucleotide Sequence Data Mining, Bioinformatics, 19, 305-306.

   Arakawa, K. and Tomita, M. (2006) G-language System as a Platform for
      large-scale analysis of high-throughput omics data, J. Pest Sci.,
      31, 7.

   Arakawa, K., Kido, N., Oshita, K., Tomita, M. (2010) G-language Genome
      Analysis Environment with REST and SOAP Web Service Interfaces,
      Nucleic Acids Res., 38, W700-W705.

</pre>

<h2>Warnings</h2>

<p>
None.
</p>

<h2>Diagnostic Error Messages</h2>

<p>
None.
</p>

<h2>Exit status</h2>

<p>
It always exits with a status of 0.
</p>

<h2>Known bugs</h2>

<p>
None.
</p>

<h2>See also</h2>

<table border cellpadding=4 bgcolor="#FFFFF0"><tr><th>Program name</th>
<th>Description</th></tr>

<tr>
<td><a href="gbase_counter.html">gbase_counter</a></td>
<td>Creates a position weight matrix of oligomers around start                  codon</td>
</tr><tr>
<td><a href="gview_cds.html">gview_cds</a></td>
<td>Displays a graph of nucleotide contents around start and stop                  codons</td>
</tr>

</table>

<h2>Author(s)</h2>

<pre>
Hidetoshi Itaya (celery@g-language.org)
  Institute for Advanced Biosciences, Keio University
  252-0882 Japan

Kazuharu Arakawa (gaou@sfc.keio.ac.jp)
  Institute for Advanced Biosciences, Keio University
  252-0882 Japan</pre>

<h2>History</h2>

   2012 - Written by Hidetoshi Itaya

<h2>Target users</h2>

   This program is intended to be used by everyone and everything, from
   naive users to embedded scrips.

<h2>Comments</h2>

   None.

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