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                                   gaminoinfo
Function

   Prints out basic amino acid sequence statistics

Description

   gaminoinfo prints out basic compositional statistics of the given amino
   acid sequence in a human readble manner. The calculated values are molecular
   weight, number of residues, average residue weight, charge, isoelectric
   point, number/mole/Dayhoffstat of each amino acid, and percentage of
   Tiny (A+C+G+S+T), Small (A+B+C+D+G+N+P+S+T+V), Aliphatic (I+L+V),
   Armoatic (F+H+W+Y), Non-polar (A+C+F+G+I+L+M+P+V+W+Y),
   Polar (D+E+H+K+N+Q+R+S+T+Z), Charged (B+D+E+H+K+R+Z), Basic (H+K+R), and
   Acidic (B+D+E+Z) reidues.
  
    
   G-language SOAP service is provided by the
   Institute for Advanced Biosciences, Keio University.
   The original web service is located at the following URL:

   http://www.g-language.org/wiki/soap

   WSDL(RPC/Encoded) file is located at:

   http://soap.g-language.org/g-language.wsdl

   Documentation on G-language Genome Analysis Environment methods are
   provided at the Document Center

   http://ws.g-language.org/gdoc/

Usage

Here is a sample session with gaminoinfo

% gaminoinfo tsw:hbb_human
Prints out basic amino acid sequence statistics
AAINDEX entry output file [hbb_human.gaminoinfo]: 

   Go to the input files for this example
   Go to the output files for this example

Command line arguments

   Standard (Mandatory) qualifiers:
  [-sequence]          seqall     Protein sequence(s) filename and optional
                                  format, or reference (input USA)
  [-outfile]           outfile    [*.gaminoinfo] AAINDEX entry output file

   Additional (Optional) qualifiers: (none)
   Advanced (Unprompted) qualifiers: (none)
   Associated qualifiers:

   "-sequence" associated qualifiers
   -sbegin1            integer    Start of each sequence to be used
   -send1              integer    End of each sequence to be used
   -sreverse1          boolean    Reverse (if DNA)
   -sask1              boolean    Ask for begin/end/reverse
   -snucleotide1       boolean    Sequence is nucleotide
   -sprotein1          boolean    Sequence is protein
   -slower1            boolean    Make lower case
   -supper1            boolean    Make upper case
   -scircular1         boolean    Sequence is circular
   -sformat1           string     Input sequence format
   -iquery1            string     Input query fields or ID list
   -ioffset1           integer    Input start position offset
   -sdbname1           string     Database name
   -sid1               string     Entryname
   -ufo1               string     UFO features
   -fformat1           string     Features format
   -fopenfile1         string     Features file name

   "-outfile" associated qualifiers
   -odirectory2        string     Output directory

   General qualifiers:
   -auto               boolean    Turn off prompts
   -stdout             boolean    Write first file to standard output
   -filter             boolean    Read first file from standard input, write
                                  first file to standard output
   -options            boolean    Prompt for standard and additional values
   -debug              boolean    Write debug output to program.dbg
   -verbose            boolean    Report some/full command line options
   -help               boolean    Report command line options and exit. More
                                  information on associated and general
                                  qualifiers can be found with -help -verbose
   -warning            boolean    Report warnings
   -error              boolean    Report errors
   -fatal              boolean    Report fatal errors
   -die                boolean    Report dying program messages
   -version            boolean    Report version number and exit

Input file format

   The database definitions for following commands are available at
   http://soap.g-language.org/kbws/embossrc

   gaminoinfo reads one or more protein sequences.

Output file format

   The output from gaminoinfo is to a plain text file.

   File: hbb_human.gaminoinfo

Sequence: P68871
AminoInfo of  from 1 to 158

Molecular weight = 19309.27 Residues = 158
Average Weight = 122.21 Charge = 3.5
Isoelectric Point = 7.4065
Residue         Number          Mole%               DayhoffStat

 =             1               0.633                     0.000
> =             1               0.633                     0.000
A = Ala         16              10.127                    1.178
B = Asx         2               1.266                     0.000
C = Cys         2               1.266                     0.436
D = Asp         7               4.430                     0.806
E = Glu         8               5.063                     0.844
F = Phe         8               5.063                     1.406
G = Gly         13              8.228                     0.980
H = His         11              6.962                     3.481
K = Lys         11              6.962                     1.055
L = Leu         18              11.392                    1.540
M = Met         3               1.899                     1.117
N = Asn         7               4.430                     1.030
P = Pro         7               4.430                     0.852
Q = Gln         3               1.899                     0.487
R = Arg         3               1.899                     0.387
S = Ser         5               3.165                     0.452
T = Thr         7               4.430                     0.726
U = Sec         1               0.633                     0.000
V = Val         18              11.392                    1.726
W = Trp         2               1.266                     0.974
Y = Tyr         3               1.899                     0.558
_ =             1               0.633                     0.000

Property        Residues                Number          Mole%
Tiny            (A+C+G+S+T)             43              27.215
Small           (A+B+C+D+G+N+P+S+T+V)   84              53.165
Aliphatic       (I+L+V)                 36              22.785
Aromatic        (F+H+W+Y)               24              15.190
Non-polar       (A+C+F+G+I+L+M+P+V+W+Y) 90              56.962
Polar           (D+E+H+K+N+Q+R+S+T+Z)   62              39.241
Charged         (B+D+E+H+K+R+Z)         42              26.582
Basic           (H+K+R)                 25              15.823
Acidic          (B+D+E+Z)               17              10.759



Data files

   None.

Notes

   None.

References

   Arakawa, K., Mori, K., Ikeda, K., Matsuzaki, T., Konayashi, Y., and
      Tomita, M. (2003) G-language Genome Analysis Environment: A Workbench
      for Nucleotide Sequence Data Mining, Bioinformatics, 19, 305-306.

   Arakawa, K. and Tomita, M. (2006) G-language System as a Platform for
      large-scale analysis of high-throughput omics data, J. Pest Sci.,
      31, 7.

   Arakawa, K., Kido, N., Oshita, K., Tomita, M. (2010) G-language Genome
      Analysis Environment with REST and SOAP Web Service Interfaces,
      Nucleic Acids Res., 38, W700-W705.

Warnings

   None.

Diagnostic Error Messages

   None.

Exit status

   It always exits with a status of 0.

Known bugs

   None.

See also

   gaaui          Calculates various indece of amino acid usage
   gcodoncompiler Calculate various kinds of amino acid and codon usage data

Author(s)

   Hidetoshi Itaya (celery@g-language.org)
   Institute for Advanced Biosciences, Keio University
   252-0882 Japan

   Kazuharu Arakawa (gaou@sfc.keio.ac.jp)
   Institute for Advanced Biosciences, Keio University
   252-0882 Japan

History

   2012 - Written by Hidetoshi Itaya
   2013 - Fixed by Hidetoshi Itaya

Target users

   This program is intended to be used by everyone and everything, from
   naive users to embedded scripts.

Comments

   None.