Mercurial > repos > kuyt002 > mummer_toolsuite
comparison MUMmer/mummer_tool.xml @ 0:61f30d177448 default tip
initial commit on Mummer toolsuite on toolshed
author | eric |
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date | Tue, 31 Mar 2015 14:19:49 +0200 |
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1 <tool id="mummer_tool" name="MUMmer compare and plot" version="0.4.alx" force_history_refresh="True"> | |
2 <description>: Compare and plot genomes (Nucmer or Promer)</description> | |
3 <command interpreter="bash"> | |
4 mummer_tool.sh | |
5 $input_ref $input_query | |
6 $out_delta $out_coords $out_png $out_log | |
7 $algorithm | |
8 $keep_delta $make_coords $keep_log $make_image | |
9 $cmd_extra | |
10 </command> | |
11 <inputs> | |
12 <param name="algorithm" type="select" format="text" value="nucmer" label="Algorithm" help="Nucmer dna or Promer protein (FASTA: protein. Dna is six frame translated)"> | |
13 <option value="nucmer">Nucmer DNA</option> | |
14 <option value="promer">Promer</option> | |
15 </param> | |
16 <param name="input_ref" type="data" format="fasta" label="Reference sequence" /> | |
17 <param name="input_query" type="data" format="fasta" label="Sequence query file"/> | |
18 <param name="make_image" type="select" format="text" value="yes" label="Generate MUMmerplot" help="MUMmerplot will be run with default settings and --large --png as fixed image."> | |
19 <option value="yes">Yes</option> | |
20 <option value="no">No</option> | |
21 </param> | |
22 <param name="keep_delta" type="select" format="text" value="no" label="Keep delta file" help="i.e. for further processing"> | |
23 <option value="no">No</option> | |
24 <option value="yes">Yes</option> | |
25 </param> | |
26 <param name="make_coords" type="select" format="text" value="yes" label="Make coords file" help="Uses the -r argument to sort lines by reference."> | |
27 <option value="no">No</option> | |
28 <option value="yes">Yes</option> | |
29 </param> | |
30 <param name="keep_log" type="select" format="text" value="no" label="Keep console log file" help="i.e. for debugging"> | |
31 <option value="no">No</option> | |
32 <option value="yes">Yes</option> | |
33 </param> | |
34 <param name="cmd_extra" type="text" size="40" value="" label="Extra cmd line options" help="the --coords is run by default" /> | |
35 </inputs> | |
36 <outputs> | |
37 <data name="out_coords" format="tabular" label="${algorithm.value_label} coords"> | |
38 <filter>make_coords=="yes"</filter> | |
39 </data> | |
40 <data name="out_delta" format="tabular" label="${algorithm.value_label} delta"> | |
41 <filter>keep_delta=="yes"</filter> | |
42 </data> | |
43 <data name="out_png" format="png" label="${algorithm.value_label} mummerplot"> | |
44 <filter>make_image=="yes"</filter> | |
45 </data> | |
46 <data name="out_log" format="tabular" label="Console log file"> | |
47 <filter>keep_log=="yes"</filter> | |
48 </data> | |
49 </outputs> | |
50 <requirements> | |
51 <requirement type="package" version="4.6">gnuplot</requirement> | |
52 <requirement type="package" version="3.23">mummer</requirement> | |
53 </requirements> | |
54 <tests> | |
55 <test> | |
56 <param name="algorithm" value="nucmer" /> | |
57 <param name="input_ref" value="test.seq1.fasta"/> | |
58 <param name="input_query" value="test.seq2.fasta"/> | |
59 <param name="make_image" value="yes"/> | |
60 <param name="keep_delta" value="no" /> | |
61 <param name="make_coords" value="no" /> | |
62 <param name="keep_log" value="yes" /> | |
63 | |
64 <output name="out_log" file="test.MUMmerplot.result.Nucmer_galaxy.log" /> | |
65 <output name="out_png" file="test.MUMmerplot.result.Nucmer_galaxy.png" /> | |
66 </test> | |
67 </tests> | |
68 <help> | |
69 | | |
70 | |
71 | |
72 **Reference** | |
73 ------------- | |
74 | |
75 - **Nucmer Galaxy tool wrapper: Alex Bossers, CVI of Wageningen UR, The Netherlands.** | |
76 | |
77 - **Nucmer or Promer of MUMmer suite:** v3.22 http://mummer.sourceforge.net/manual/ | |
78 | |
79 - **MUMmer tutorials:** http://mummer.sourceforge.net/examples/ | |
80 | |
81 | |
82 If you found these tools/wrappers useful in your research, please acknowledge our work. If you improve | |
83 or modify the wrappers please add instead of substitute yourself into the acknowlegement section :) | |
84 | |
85 | |
86 **Command line arguments** | |
87 -------------------------- | |
88 | |
89 --mum Use anchor matches that are unique in both the reference and query | |
90 --mumreference Use anchor matches that are unique in the reference but not necessarily unique in the query (default behavior) | |
91 --maxmatch Use all anchor matches regardless of their uniqueness | |
92 --breaklen Distance an alignment extension will attempt to extend poor scoring regions before giving up (default 200) | |
93 --mincluster Minimum cluster length (default 65) | |
94 --delta Toggle the creation of the delta file. Setting --nodelta prevents the alignment extension step and only outputs the match clusters (default --delta) | |
95 --depend Print the dependency information and exit | |
96 --diagfactor Maximum diagonal difference factor for clustering, i.e. diagonal difference / match separation (default 0.12) | |
97 --extend Toggle the outward extension of alignments from their anchoring clusters. Setting --noextend will prevent alignment extensions but still align the DNA between clustered matches and create the .delta file (default --extend) | |
98 --forward Align only the forward strands of each sequence | |
99 --maxgap Maximum gap between two adjacent matches in a cluster (default 90) | |
100 --help Print the help information and exit | |
101 --minmatch Minimum length of an maximal exact match (default 20) | |
102 --optimize Toggle alignment score optimization. Setting --nooptimize will prevent alignment score optimization and result in sometimes longer, but lower scoring alignments (default --optimize) | |
103 --reverse Align only the reverse strand of the query sequence to the forward strand of the reference | |
104 --simplify Simplify alignments by removing shadowed clusters. Turn this option off (--nosimplify) if aligning a sequence to itself to look for repeats (default --simplify) | |
105 --version Print the version information and exit | |
106 --coords **Automatically ON in galaxy wrapper!** It generates the .coords file using the 'show-coords' program with the -r option. | |
107 --prefix **Do NOT use in Galaxy wrapper!** Set the output file prefix (default out) | |
108 | |
109 | | |
110 | | |
111 | |
112 </help> | |
113 </tool> | |
114 |