Mercurial > repos > kyost > atac_primer_tool
diff window_coverage.xml @ 0:fd3ea97a96bc draft
planemo upload commit 103cb51ec368438642504c3f98b76c363db478bb
author | kyost |
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date | Sat, 28 Apr 2018 15:07:26 -0400 |
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--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/window_coverage.xml Sat Apr 28 15:07:26 2018 -0400 @@ -0,0 +1,38 @@ +<tool id="window_coverage" name="Calculate window coverage" version="0.1.0"> + <description> Determines spanning and total read coverage for overlapping windows spanning peaks of interest. </description> + <requirements> + <requirement type="package" version="1.3">samtools</requirement> + <requirement type="package" version="2.27.0.0">bedtools</requirement> + </requirements> + <stdio> + <exit_code range="1:" /> + </stdio> + <command><![CDATA[ + #set input_list = '","'.join( [str( $input.name ) for $input in $input1 ] ) + $__tool_directory__/./window_coverage.bash "$input1" "$input_list" "$input2" "$input3" "$output1" "$output2" + ]]></command> + <inputs> + <param type="data" name="input1" format="bam" multiple="true" label="Filtered Bam Files"/> + <param type="data" name="input2" format="bed" label="qPCR Window Bed File"/> + <param type="float" name="input3" value="0.9" label="Coverage Cutoff"/> + </inputs> + <outputs> + <data name="output1" format="bed" label="Coverage Bed File"/> + <data name="output2" format="bed" label="Spanning Fragment Coverage Bed File" /> + </outputs> + <tests> + <test> + <param name="input1" value="Ch1-1.pe.q10.sort.bam.rmdup.filter.bam,Ch13-1.pe.q10.sort.rmdup.filter.bam"/> + <param name="input2" value="qPCR_windows.bed" /> + <param name="input3" value="0.9" /> + <output name="output1" file="combined.o.coverage"/> + <output name="output2" file="combined.f9.coverage"/> + </test> + </tests> + <help><![CDATA[ + This tools takes a series of bam files and a bed file of overlapping windows spanning peaks of interest and determines the read coverage and spanning fragment coverage for each window. + ]]></help> + <citations> + <citation type="doi">doi:10.1038/nmeth.4663</citation> + </citations> +</tool>