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planemo upload commit 89aa8abdc88aa922cab8d9c5f17078ba1e388339-dirty
author ldelisle
date Wed, 19 Oct 2022 08:34:43 +0000
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0:07695b197e6c 1:2c86ad672f52
1 <tool id="revertR2orientationInBam" name="revertR2orientationInBam" version="0.0.1" profile="16.04">
2 <description> Revert the mapped orientation of R2 mates in a bam.</description>
3 <requirements>
4 <requirement type="package" version="1.9">samtools</requirement>
5 </requirements>
6 <stdio>
7 <!-- Anything other than zero is an error -->
8 <exit_code range="1:" />
9 <exit_code range=":-1" />
10 <!-- In case the return code has not been set propery check stderr too -->
11 <regex match="Error:" />
12 <regex match="Exception:" />
13 </stdio>
14 <command>
15 <![CDATA[
16 bash $__tool_directory__/revertR2orientationInBam.sh $input $output
17 ]]>
18 </command>
19 <inputs>
20 <!-- note unsorted bam includes all derived bam types (inlcuding bam which is sorted) -->
21 <param name="input" type="data" format="sam,bam" label="Select a sam or bam file."/>
22 </inputs>
23 <outputs>
24 <data format="bam" name="output" label="$input.name with R2 orientation reversed"/>
25 </outputs>
26
27 <tests>
28 <test>
29 <param name="input" value="input.sam"/>
30 <output name="output" file="output.bam" ftype="bam"/>
31 </test>
32 </tests>
33 <help>
34 <![CDATA[
35 This tool is very useful when you have paired-end stranded RNA-seq.
36 Using this tool prior to a bedtools genome coverage allow to have strand specific coverage using both mates.
37 It uses samtools to convert input to sam format and then awk to modify the flag "reverse strand" for the second mate of pairs.
38 ]]>
39 </help>
40 </tool>