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1 <tool id="rnammer" name="rnammer" version="0.1">
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2 <description>RNAmmer predicts 5S/8S, 16S/18S, and 23S/28S ribosomal RNA in genome sequences.</description>
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3 <requirements>
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4 <requirement type="package" version="1.2">rnammer</requirement>
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5 <requirement type="package" version="2.3.2">hmmer</requirement>
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6 </requirements>
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7 <command>rnammer -S $kingdom -m $molecules -xml $out_xml -gff $out_gff -f $out_fasta -h $out_hmm_report
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8 #if $multi
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9 -multi
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10 #end if
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11 $in_fasta
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12 </command>
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13 <inputs>
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14 <param name="in_fasta" type="data" label="Input sequence (fasta)" help="Genome sequence" />
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15 <param name="kingdom" type="select" label="Domain" help="Which domain of Life does your sequence belong to." force_select="true" multiple="false" display="radio">
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16 <option value="arc">Archaea</option>
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17 <option value="bac" selected="true">Bacteria</option>
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18 <option value="euk">Eukaryotes</option>
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19 </param>
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20 <param name="molecules" type="select" label="Molecules" help="Which rRNAs to detect." force_select="true" multiple="true" display="checkboxes">
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21 <option value="tsu" selected="true">TSU: 5S/8S</option>
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22 <option value="ssu" selected="true">SSU: 16S/18S</option>
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23 <option value="lsu" selected="true">LSU: 23S/28S</option>
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24 </param>
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25 <param name="multi" type="boolean" label="Run in parallel?" help="Runs all molecules and both strands in parallel. Require 6 threads." truevalue="true" falsevalue="false" checked="true" />
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26 </inputs>
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27 <outputs>
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28 <data name="out_xml" format="xml" label="rRNA molecules (xml)" />
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29 <data name="out_gff" format="gff" label="rRNA molecules (GFF)" />
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30 <data name="out_fasta" format="fasta" label="Predicted rRNA genes (fasta)" />
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31 <data name="out_hmm_report" format="txt" label="HMM report" />
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32 </outputs>
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33 <help>
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34 **What it does**
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35
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36 Runs rnammer, a wrapper that makes use of hmmer to identify ribosomal RNA genes on full or draft genomes.
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37
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38 **Citation**
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39
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40 Lagesen K, Hallin PF, Roedland E, Staerfeldt HH, Rognes T, Ussery DW. RNammer: consistent annotation of rRNA genes in genomic sequences. Nucleic Acids Res. 2007 Apr 22.
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41
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42 </help>
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43 </tool>
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