Mercurial > repos > lionelguy > spades
view tools/spades_2_5/spades.pl @ 4:a0ea0f7ae029 draft
Fixed the version number in tool_deps
author | lionelguy |
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date | Thu, 12 Sep 2013 07:48:30 -0400 |
parents | b5ce24f34dd7 |
children | 95ddc2380130 |
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#!/usr/bin/env perl ## A wrapper script to call spades.py and collect its output use strict; use warnings; use File::Temp qw/ tempfile tempdir /; use File::Copy; use Getopt::Long; # Parse arguments my ($out_contigs_file, $out_contigs_stats, $out_scaffolds_file, $out_scaffolds_stats, $out_log_file, @sysargs) = @ARGV; ## GetOptions not compatible with parsing the rest of the arguments in an array. ## Keeping the not-so-nice parse-in-one-go method, without named arguments. # GetOptions( # 'contigs-file=s' => \$out_contigs_file, # 'contigs-stats=s' => \$out_contigs_stats, # 'scaffolds-file=s' => \$out_scaffolds_file, # 'scaffolds-stats=s' => \$out_scaffolds_stats, # 'out_log_file=s' => \$out_log_file, # ); # my @sysargs = @ARGV; # Create temporary folder to store files, delete after use #my $output_dir = tempdir( CLEANUP => 0 ); my $output_dir = 'output_dir'; # Link "dat" files as fastq, otherwise spades complains about file format # Create log handle open my $log, '>', $out_log_file or die "Cannot write to $out_log_file: $?\n"; # Run program # To do: record time &runSpades(@sysargs); &collectOutput(); &extractCoverageLength($out_contigs_file, $out_contigs_stats); &extractCoverageLength($out_scaffolds_file, $out_scaffolds_stats); print $log "Done\n"; close $log; exit 0; # Run spades sub runSpades { my $cmd = join(" ", @_) . " -o $output_dir"; my $return_code = system($cmd); if ($return_code) { print $log "Failed with code $return_code\nCommand $cmd\nMessage: $?\n"; die "Failed with code $return_code\nCommand $cmd\nMessage: $?\n"; } return 0; } # Collect output sub collectOutput{ # To do: check that the files are there # Collects output move "$output_dir/contigs.fasta", $out_contigs_file; move "$output_dir/scaffolds.fasta", $out_scaffolds_file; open LOG, '<', "$output_dir/spades.log" or die "Cannot open log file $output_dir/spades.log: $?"; print $log $_ while (<LOG>); return 0; } # Extract sub extractCoverageLength{ my ($in, $out) = @_; open FASTA, '<', $in or die $!; open TAB, '>', $out or die $!; print TAB "#name\tlength\tcoverage\n"; while (<FASTA>){ next unless /^>/; chomp; my @a = split(/\s/, $_); my ($NODE, $n, $LENGTH, $l, $COV, $cov) = split(/_/, $a[0]); die "Not all elements found in $_\n" unless ($n && $l && $cov); print TAB "NODE_$n\t$l\t$cov\n"; } close TAB; }