Mercurial > repos > lldelisle > revertr2orientationinbam
view revertR2orientationInBam.sh @ 0:05aa21d39189 draft
planemo upload for repository https://github.com/lldelisle/tools-lldelisle/tree/master/tools/revertR2orientationInBam commit fe9813fe41ba241b47f7b2b4e4c9672ef35c0b35-dirty
author | lldelisle |
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date | Wed, 19 Oct 2022 08:50:18 +0000 |
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#!/bin/bash inputBam=$1 outputBam=$2 samtools view -h "$inputBam" | awk -v OFS="\t" '{ # Process only non header lines if(!($1~/^@/)){ # SAM flag is field 2 n=$2 # Change only the second in pair flag is 128 d=128 q=(n-n%d)/d+(n<0) if(q%2==1){ # Evaluate the strand reverse strand flag is 16 d=16 q=(n-n%d)/d+(n<0) if(q%2==1){ # It is reverse it is now forward $2-=16 }else{ # It is forward it is now reverse $2+=16 } } } print }' | samtools view -b - > "$outputBam"