Mercurial > repos > lparsons > fastx_barcode_splitter_enhanced
annotate fastx_barcode_splitter.pl @ 1:b7b3d008e2d3 draft
planemo upload for repository https://github.com/lparsons/galaxy_tools/tree/master/tools/fastx_barcode_splitter_enhanced commit e6a486662201362134119c0ddc7151b8dd181ebd
author | lparsons |
---|---|
date | Tue, 27 Oct 2015 11:47:52 -0400 |
parents | 84bbf4fd24c3 |
children | e7b7cdc1834d |
rev | line source |
---|---|
0
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
1 #!/usr/bin/perl |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
2 |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
3 # FASTX-toolkit - FASTA/FASTQ preprocessing tools. |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
4 # Copyright (C) 2009 A. Gordon (gordon@cshl.edu) |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
5 # |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
6 # Lance Parsons (lparsons@princeton.edu) |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
7 # 3/21/2011 - Modified to accept separate index file for barcodes |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
8 # 4/6/2011 - Modified to cleanup bad barcode identifiers (esp. useful for Galaxy) |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
9 # |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
10 # This program is free software: you can redistribute it and/or modify |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
11 # it under the terms of the GNU Affero General Public License as |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
12 # published by the Free Software Foundation, either version 3 of the |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
13 # License, or (at your option) any later version. |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
14 # |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
15 # This program is distributed in the hope that it will be useful, |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
16 # but WITHOUT ANY WARRANTY; without even the implied warranty of |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
17 # MERCHANTABILITY or FITNESS FOR A PARTICULAR PURPOSE. See the |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
18 # GNU Affero General Public License for more details. |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
19 # |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
20 # You should have received a copy of the GNU Affero General Public License |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
21 # along with this program. If not, see <http://www.gnu.org/licenses/>. |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
22 |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
23 use strict; |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
24 use warnings; |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
25 use IO::Handle; |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
26 use Data::Dumper; |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
27 use Getopt::Long; |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
28 use Carp; |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
29 |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
30 ## |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
31 ## This program splits a FASTQ/FASTA file into several smaller files, |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
32 ## Based on barcode matching. |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
33 ## |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
34 ## run with "--help" for usage information |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
35 ## |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
36 ## Assaf Gordon <gordon@cshl.edu> , 11sep2008 |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
37 |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
38 # Forward declarations |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
39 sub load_barcode_file ($); |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
40 sub parse_command_line ; |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
41 sub match_sequences ; |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
42 sub mismatch_count($$) ; |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
43 sub print_results; |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
44 sub open_and_detect_input_format; |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
45 sub open_index_and_detect_input_format($); |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
46 sub read_index_record; |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
47 sub read_record; |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
48 sub write_record($); |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
49 sub usage(); |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
50 |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
51 # Global flags and arguments, |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
52 # Set by command line argumens |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
53 my $barcode_file ; |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
54 my $barcodes_at_eol = 0 ; |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
55 my $barcodes_at_bol = 0 ; |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
56 my $index_read_file ; |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
57 my $exact_match = 0 ; |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
58 my $allow_partial_overlap = 0; |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
59 my $allowed_mismatches = 1; |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
60 my $newfile_suffix = ''; |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
61 my $newfile_prefix ; |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
62 my $quiet = 0 ; |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
63 my $debug = 0 ; |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
64 my $fastq_format = 1; |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
65 my $index_fastq_format = 1; |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
66 my $read_id_check_strip_characters = 1; |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
67 |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
68 # Global variables |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
69 # Populated by 'create_output_files' |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
70 my %filenames; |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
71 my %files; |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
72 my %counts = ( 'unmatched' => 0 ); |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
73 my $barcodes_length; |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
74 my @barcodes; |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
75 my $input_file_io; |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
76 |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
77 |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
78 # The Four lines per record in FASTQ format. |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
79 # (when using FASTA format, only the first two are used) |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
80 my $seq_name; |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
81 my $seq_bases; |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
82 my $seq_name2; |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
83 my $seq_qualities; |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
84 |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
85 # Values used for index read file |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
86 my $index_seq_name; |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
87 my $index_seq_bases; |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
88 my $index_seq_name2; |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
89 my $index_seq_qualities; |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
90 |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
91 |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
92 |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
93 # |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
94 # Start of Program |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
95 # |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
96 parse_command_line ; |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
97 |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
98 load_barcode_file ( $barcode_file ) ; |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
99 |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
100 open_and_detect_input_format; |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
101 |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
102 if (defined $index_read_file) {open_index_and_detect_input_format ( $index_read_file );} |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
103 |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
104 match_sequences ; |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
105 |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
106 print_results unless $quiet; |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
107 |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
108 # |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
109 # End of program |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
110 # |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
111 |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
112 |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
113 |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
114 |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
115 |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
116 |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
117 |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
118 |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
119 sub parse_command_line { |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
120 my $help; |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
121 |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
122 usage() if (scalar @ARGV==0); |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
123 |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
124 my $result = GetOptions ( "bcfile=s" => \$barcode_file, |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
125 "eol" => \$barcodes_at_eol, |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
126 "bol" => \$barcodes_at_bol, |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
127 "idxfile=s" => \$index_read_file, |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
128 "idxidstrip=i" => \$read_id_check_strip_characters, |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
129 "exact" => \$exact_match, |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
130 "prefix=s" => \$newfile_prefix, |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
131 "suffix=s" => \$newfile_suffix, |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
132 "quiet" => \$quiet, |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
133 "partial=i" => \$allow_partial_overlap, |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
134 "debug" => \$debug, |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
135 "mismatches=i" => \$allowed_mismatches, |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
136 "help" => \$help |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
137 ) ; |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
138 |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
139 usage() if ($help); |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
140 |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
141 die "Error: barcode file not specified (use '--bcfile [FILENAME]')\n" unless defined $barcode_file; |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
142 die "Error: prefix path/filename not specified (use '--prefix [PATH]')\n" unless defined $newfile_prefix; |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
143 |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
144 if (! defined $index_read_file) { |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
145 if ($barcodes_at_bol == $barcodes_at_eol) { |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
146 die "Error: can't specify both --eol & --bol\n" if $barcodes_at_eol; |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
147 die "Error: must specify either --eol or --bol or --idxfile\n" ; |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
148 } |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
149 } |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
150 elsif ($barcodes_at_bol || $barcodes_at_eol) { |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
151 die "Error: Must specify only one of --idxfile, --eol, or --bol"; |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
152 } |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
153 |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
154 die "Error: invalid for value partial matches (valid values are 0 or greater)\n" if $allow_partial_overlap<0; |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
155 |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
156 $allowed_mismatches = 0 if $exact_match; |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
157 |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
158 die "Error: invalid value for mismatches (valid values are 0 or more)\n" if ($allowed_mismatches<0); |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
159 |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
160 die "Error: partial overlap value ($allow_partial_overlap) bigger than " . |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
161 "max. allowed mismatches ($allowed_mismatches)\n" if ($allow_partial_overlap > $allowed_mismatches); |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
162 |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
163 |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
164 exit unless $result; |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
165 } |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
166 |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
167 |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
168 |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
169 # |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
170 # Read the barcode file |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
171 # |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
172 sub load_barcode_file ($) { |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
173 my $filename = shift or croak "Missing barcode file name"; |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
174 |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
175 open BCFILE,"<$filename" or die "Error: failed to open barcode file ($filename)\n"; |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
176 while (<BCFILE>) { |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
177 next if m/^#/; |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
178 chomp; |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
179 my ($ident, $barcode) = split('\t') ; |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
180 |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
181 $barcode = uc($barcode); |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
182 |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
183 # Sanity checks on the barcodes |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
184 die "Error: bad data at barcode file ($filename) line $.\n" unless defined $barcode; |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
185 die "Error: bad barcode value ($barcode) at barcode file ($filename) line $.\n" |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
186 unless $barcode =~ m/^[AGCT]+$/; |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
187 |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
188 # Cleanup Identifiers (only allow alphanumeric, replace others with dash '-') |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
189 $ident =~ s/[^A-Za-z0-9]/-/g; |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
190 die "Error: bad identifier value ($ident) at barcode file ($filename) line $. (must be alphanumeric)\n" |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
191 unless $ident =~ m/^[A-Za-z0-9-]+$/; |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
192 |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
193 die "Error: badcode($ident, $barcode) is shorter or equal to maximum number of " . |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
194 "mismatches ($allowed_mismatches). This makes no sense. Specify fewer mismatches.\n" |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
195 if length($barcode)<=$allowed_mismatches; |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
196 |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
197 $barcodes_length = length($barcode) unless defined $barcodes_length; |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
198 die "Error: found barcodes in different lengths. this feature is not supported yet.\n" |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
199 unless $barcodes_length == length($barcode); |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
200 |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
201 push @barcodes, [$ident, $barcode]; |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
202 |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
203 if ($allow_partial_overlap>0) { |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
204 foreach my $i (1 .. $allow_partial_overlap) { |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
205 substr $barcode, ($barcodes_at_bol)?0:-1, 1, ''; |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
206 push @barcodes, [$ident, $barcode]; |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
207 } |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
208 } |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
209 } |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
210 close BCFILE; |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
211 |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
212 if ($debug) { |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
213 print STDERR "barcode\tsequence\n"; |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
214 foreach my $barcoderef (@barcodes) { |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
215 my ($ident, $seq) = @{$barcoderef}; |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
216 print STDERR $ident,"\t", $seq ,"\n"; |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
217 } |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
218 } |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
219 } |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
220 |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
221 # Create one output file for each barcode. |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
222 # (Also create a file for the dummy 'unmatched' barcode) |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
223 sub create_output_files { |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
224 my %barcodes = map { $_->[0] => 1 } @barcodes; #generate a uniq list of barcode identifiers; |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
225 $barcodes{'unmatched'} = 1 ; |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
226 |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
227 foreach my $ident (keys %barcodes) { |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
228 my $new_filename = $newfile_prefix . $ident . $newfile_suffix; |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
229 $filenames{$ident} = $new_filename; |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
230 open my $file, ">$new_filename" or die "Error: failed to create output file ($new_filename)\n"; |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
231 $files{$ident} = $file ; |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
232 } |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
233 } |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
234 |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
235 sub match_sequences { |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
236 |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
237 my %barcodes = map { $_->[0] => 1 } @barcodes; #generate a uniq list of barcode identifiers; |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
238 $barcodes{'unmatched'} = 1 ; |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
239 |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
240 #reset counters |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
241 foreach my $ident ( keys %barcodes ) { |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
242 $counts{$ident} = 0; |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
243 } |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
244 |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
245 create_output_files; |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
246 |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
247 # Read file FASTQ file |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
248 # split accotding to barcodes |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
249 while ( read_record ) { |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
250 chomp $seq_name; |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
251 chomp $seq_bases; |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
252 if (defined $index_read_file) { |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
253 read_index_record() or die "Error: Unable to read index sequence for sequence name ($seq_name), check to make sure the file lengths match.\n"; |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
254 chomp $index_seq_name; |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
255 chomp $index_seq_bases; |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
256 |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
257 # Assert that the read ids match |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
258 my $seq_name_match = &strip_read_id($seq_name); |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
259 my $index_seq_name_match = &strip_read_id($index_seq_name); |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
260 if ($seq_name_match ne $index_seq_name_match) { |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
261 die "Error: Index sequence name ($index_seq_name) does not match sequence name ($seq_name)\n"; |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
262 } |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
263 |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
264 } |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
265 |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
266 print STDERR "sequence $seq_bases: \n" if $debug; |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
267 |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
268 my $best_barcode_mismatches_count = $barcodes_length; |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
269 my $best_barcode_ident = undef; |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
270 |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
271 #Try all barcodes, find the one with the lowest mismatch count |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
272 foreach my $barcoderef (@barcodes) { |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
273 my ($ident, $barcode) = @{$barcoderef}; |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
274 |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
275 # Get DNA fragment (in the length of the barcodes) |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
276 # The barcode will be tested only against this fragment |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
277 # (no point in testing the barcode against the whole sequence) |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
278 my $sequence_fragment; |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
279 if ($barcodes_at_bol) { |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
280 $sequence_fragment = substr $seq_bases, 0, $barcodes_length; |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
281 } elsif ($barcodes_at_eol) { |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
282 $sequence_fragment = substr $seq_bases, - $barcodes_length; |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
283 } else { |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
284 $sequence_fragment = substr $index_seq_bases, 0, $barcodes_length; |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
285 } |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
286 |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
287 my $mm = mismatch_count($sequence_fragment, $barcode) ; |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
288 |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
289 # if this is a partial match, add the non-overlap as a mismatch |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
290 # (partial barcodes are shorter than the length of the original barcodes) |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
291 $mm += ($barcodes_length - length($barcode)); |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
292 |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
293 if ( $mm < $best_barcode_mismatches_count ) { |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
294 $best_barcode_mismatches_count = $mm ; |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
295 $best_barcode_ident = $ident ; |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
296 } |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
297 } |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
298 |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
299 $best_barcode_ident = 'unmatched' |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
300 if ( (!defined $best_barcode_ident) || $best_barcode_mismatches_count>$allowed_mismatches) ; |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
301 |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
302 print STDERR "sequence $seq_bases matched barcode: $best_barcode_ident\n" if $debug; |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
303 |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
304 $counts{$best_barcode_ident}++; |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
305 |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
306 #get the file associated with the matched barcode. |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
307 #(note: there's also a file associated with 'unmatched' barcode) |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
308 my $file = $files{$best_barcode_ident}; |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
309 |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
310 write_record($file); |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
311 } |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
312 } |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
313 |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
314 # Strip end of readids when matching to avoid mismatch between read 1, 2, 3, etc. |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
315 sub strip_read_id { |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
316 my $read_id = shift; |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
317 my $stripped_read_id = $read_id; |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
318 if ($read_id_check_strip_characters) { |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
319 if ($read_id =~ /@([a-zA-Z0-9_-]+):([0-9]+):([a-zA-Z0-9]+):([0-9]+):([0-9]+):([0-9]+):([0-9]+) ([0-9]+):([YN]):([0-9]+):([ACGT]+){0,1}/) { # CASAVA 1.8+ |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
320 my @parts = split(/ /,$read_id); |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
321 $stripped_read_id = $parts[0]; |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
322 } else { # CASAVA 1.7 and earlier |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
323 $stripped_read_id = substr($read_id, 0, length($read_id)-$read_id_check_strip_characters); |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
324 } |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
325 } |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
326 return $stripped_read_id; |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
327 } |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
328 |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
329 |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
330 #Quickly calculate hamming distance between two strings |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
331 # |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
332 #NOTE: Strings must be same length. |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
333 # returns number of different characters. |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
334 #see http://www.perlmonks.org/?node_id=500235 |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
335 sub mismatch_count($$) { length( $_[ 0 ] ) - ( ( $_[ 0 ] ^ $_[ 1 ] ) =~ tr[\0][\0] ) } |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
336 |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
337 |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
338 |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
339 sub print_results |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
340 { |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
341 print "Barcode\tCount\tLocation\n"; |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
342 my $total = 0 ; |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
343 foreach my $ident (sort keys %counts) { |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
344 print $ident, "\t", $counts{$ident},"\t",$filenames{$ident},"\n"; |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
345 $total += $counts{$ident}; |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
346 } |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
347 print "total\t",$total,"\n"; |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
348 } |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
349 |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
350 |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
351 sub read_record |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
352 { |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
353 $seq_name = $input_file_io->getline(); |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
354 |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
355 return undef unless defined $seq_name; # End of file? |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
356 |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
357 $seq_bases = $input_file_io->getline(); |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
358 die "Error: bad input file, expecting line with sequences\n" unless defined $seq_bases; |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
359 |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
360 # If using FASTQ format, read two more lines |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
361 if ($fastq_format) { |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
362 $seq_name2 = $input_file_io->getline(); |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
363 die "Error: bad input file, expecting line with sequence name2\n" unless defined $seq_name2; |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
364 |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
365 $seq_qualities = $input_file_io->getline(); |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
366 die "Error: bad input file, expecting line with quality scores\n" unless defined $seq_qualities; |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
367 } |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
368 return 1; |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
369 } |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
370 |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
371 sub write_record($) |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
372 { |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
373 my $file = shift; |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
374 |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
375 croak "Bad file handle" unless defined $file; |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
376 |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
377 print $file $seq_name,"\n"; |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
378 print $file $seq_bases,"\n"; |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
379 |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
380 #if using FASTQ format, write two more lines |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
381 if ($fastq_format) { |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
382 print $file $seq_name2; |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
383 print $file $seq_qualities; |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
384 } |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
385 } |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
386 |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
387 sub open_and_detect_input_format |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
388 { |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
389 $input_file_io = new IO::Handle; |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
390 die "Failed to open STDIN " unless $input_file_io->fdopen(fileno(STDIN),"r"); |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
391 |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
392 # Get the first characeter, and push it back |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
393 my $first_char = $input_file_io->getc(); |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
394 $input_file_io->ungetc(ord $first_char); |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
395 |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
396 if ($first_char eq '>') { |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
397 # FASTA format |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
398 $fastq_format = 0 ; |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
399 print STDERR "Detected FASTA format\n" if $debug; |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
400 } elsif ($first_char eq '@') { |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
401 # FASTQ format |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
402 $fastq_format = 1; |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
403 print STDERR "Detected FASTQ format\n" if $debug; |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
404 } else { |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
405 die "Error: unknown file format. First character = '$first_char' (expecting > or \@)\n"; |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
406 } |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
407 } |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
408 |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
409 |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
410 sub open_index_and_detect_input_format($) { |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
411 my $filename = shift or croak "Missing index read file name"; |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
412 |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
413 open IDXFILE,"<$filename" or die "Error: failed to open index read file ($filename)\n"; |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
414 |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
415 # Get the first line, and reset file pointer |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
416 my $first_line = <IDXFILE>; |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
417 my $first_char = substr($first_line, 0, 1); |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
418 seek(IDXFILE, 0, 0); |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
419 |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
420 if ($first_char eq '>') { |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
421 # FASTA format |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
422 $index_fastq_format = 0 ; |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
423 print STDERR "Detected FASTA format for index file\n" if $debug; |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
424 } elsif ($first_char eq '@') { |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
425 # FASTQ format |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
426 $index_fastq_format = 1; |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
427 print STDERR "Detected FASTQ format for index file\n" if $debug; |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
428 } else { |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
429 die "Error: unknown index file format. First character = '$first_char' (expecting > or \@)\n"; |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
430 } |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
431 } |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
432 |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
433 sub read_index_record |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
434 { |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
435 $index_seq_name = <IDXFILE>; |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
436 |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
437 return undef unless defined $index_seq_name; # End of file? |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
438 |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
439 $index_seq_bases = <IDXFILE>; |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
440 die "Error: bad input file, expecting line with sequences\n" unless defined $index_seq_bases; |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
441 |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
442 # If using FASTQ format, read two more lines |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
443 if ($index_fastq_format) { |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
444 $index_seq_name2 = <IDXFILE>; |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
445 die "Error: bad input file, expecting line with sequence name2\n" unless defined $index_seq_name2; |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
446 |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
447 $index_seq_qualities = <IDXFILE>; |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
448 die "Error: bad input file, expecting line with quality scores\n" unless defined $index_seq_qualities; |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
449 } |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
450 return 1; |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
451 } |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
452 |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
453 sub usage() |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
454 { |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
455 print<<EOF; |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
456 Barcode Splitter, by Assaf Gordon (gordon\@cshl.edu), 11sep2008 |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
457 |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
458 This program reads FASTA/FASTQ file and splits it into several smaller files, |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
459 Based on barcode matching. |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
460 FASTA/FASTQ data is read from STDIN (format is auto-detected.) |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
461 Output files will be writen to disk. |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
462 Summary will be printed to STDOUT. |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
463 |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
464 usage: $0 --bcfile FILE --prefix PREFIX [--suffix SUFFIX] [--bol|--eol|--idxfile] |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
465 [--mismatches N] [--exact] [--partial N] [--idxidstrip N] |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
466 [--help] [--quiet] [--debug] |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
467 |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
468 Arguments: |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
469 |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
470 --bcfile FILE - Barcodes file name. (see explanation below.) |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
471 --prefix PREFIX - File prefix. will be added to the output files. Can be used |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
472 to specify output directories. |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
473 --suffix SUFFIX - File suffix (optional). Can be used to specify file |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
474 extensions. |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
475 --bol - Try to match barcodes at the BEGINNING of sequences. |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
476 (What biologists would call the 5' end, and programmers |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
477 would call index 0.) |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
478 --eol - Try to match barcodes at the END of sequences. |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
479 (What biologists would call the 3' end, and programmers |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
480 would call the end of the string.) |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
481 --idxfile FILE - Read barcodes from separate index file (fasta or fastq) |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
482 NOTE: one of --bol, --eol, --idxfile must be specified, |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
483 but not more than one. |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
484 --idxidstrip N - When using index file, strip this number of characters |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
485 from the end of the sequence id before matching. |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
486 Automatically detects CASAVA 1.8 format and strips at a |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
487 space in the id, use 0 to disable this. |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
488 (Default is 1). |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
489 --mismatches N - Max. number of mismatches allowed. default is 1. |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
490 --exact - Same as '--mismatches 0'. If both --exact and --mismatches |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
491 are specified, '--exact' takes precedence. |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
492 --partial N - Allow partial overlap of barcodes. (see explanation below.) |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
493 (Default is not partial matching) |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
494 --quiet - Don't print counts and summary at the end of the run. |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
495 (Default is to print.) |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
496 --debug - Print lots of useless debug information to STDERR. |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
497 --help - This helpful help screen. |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
498 |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
499 Example (Assuming 's_2_100.txt' is a FASTQ file, 'mybarcodes.txt' is |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
500 the barcodes file): |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
501 |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
502 \$ cat s_2_100.txt | $0 --bcfile mybarcodes.txt --bol --mismatches 2 \\ |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
503 --prefix /tmp/bla_ --suffix ".txt" |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
504 |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
505 Barcode file format |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
506 ------------------- |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
507 Barcode files are simple text files. Each line should contain an identifier |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
508 (descriptive name for the barcode), and the barcode itself (A/C/G/T), |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
509 separated by a TAB character. Example: |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
510 |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
511 #This line is a comment (starts with a 'number' sign) |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
512 BC1 GATCT |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
513 BC2 ATCGT |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
514 BC3 GTGAT |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
515 BC4 TGTCT |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
516 |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
517 For each barcode, a new FASTQ file will be created (with the barcode's |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
518 identifier as part of the file name). Sequences matching the barcode |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
519 will be stored in the appropriate file. |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
520 |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
521 Running the above example (assuming "mybarcodes.txt" contains the above |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
522 barcodes), will create the following files: |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
523 /tmp/bla_BC1.txt |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
524 /tmp/bla_BC2.txt |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
525 /tmp/bla_BC3.txt |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
526 /tmp/bla_BC4.txt |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
527 /tmp/bla_unmatched.txt |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
528 The 'unmatched' file will contain all sequences that didn't match any barcode. |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
529 |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
530 Barcode matching |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
531 ---------------- |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
532 |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
533 ** Without partial matching: |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
534 |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
535 Count mismatches between the FASTA/Q sequences and the barcodes. |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
536 The barcode which matched with the lowest mismatches count (providing the |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
537 count is small or equal to '--mismatches N') 'gets' the sequences. |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
538 |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
539 Example (using the above barcodes): |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
540 Input Sequence: |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
541 GATTTACTATGTAAAGATAGAAGGAATAAGGTGAAG |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
542 |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
543 Matching with '--bol --mismatches 1': |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
544 GATTTACTATGTAAAGATAGAAGGAATAAGGTGAAG |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
545 GATCT (1 mismatch, BC1) |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
546 ATCGT (4 mismatches, BC2) |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
547 GTGAT (3 mismatches, BC3) |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
548 TGTCT (3 mismatches, BC4) |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
549 |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
550 This sequence will be classified as 'BC1' (it has the lowest mismatch count). |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
551 If '--exact' or '--mismatches 0' were specified, this sequence would be |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
552 classified as 'unmatched' (because, although BC1 had the lowest mismatch count, |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
553 it is above the maximum allowed mismatches). |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
554 |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
555 Matching with '--eol' (end of line) does the same, but from the other side |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
556 of the sequence. |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
557 |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
558 ** With partial matching (very similar to indels): |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
559 |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
560 Same as above, with the following addition: barcodes are also checked for |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
561 partial overlap (number of allowed non-overlapping bases is '--partial N'). |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
562 |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
563 Example: |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
564 Input sequence is ATTTACTATGTAAAGATAGAAGGAATAAGGTGAAG |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
565 (Same as above, but note the missing 'G' at the beginning.) |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
566 |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
567 Matching (without partial overlapping) against BC1 yields 4 mismatches: |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
568 ATTTACTATGTAAAGATAGAAGGAATAAGGTGAAG |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
569 GATCT (4 mismatches) |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
570 |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
571 Partial overlapping would also try the following match: |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
572 -ATTTACTATGTAAAGATAGAAGGAATAAGGTGAAG |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
573 GATCT (1 mismatch) |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
574 |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
575 Note: scoring counts a missing base as a mismatch, so the final |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
576 mismatch count is 2 (1 'real' mismatch, 1 'missing base' mismatch). |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
577 If running with '--mismatches 2' (meaning allowing upto 2 mismatches) - this |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
578 seqeunce will be classified as BC1. |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
579 |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
580 EOF |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
581 |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
582 exit 1; |
84bbf4fd24c3
Initial toolshed version with support for separate index reads and automatic loading of results into Galaxy history.
lparsons
parents:
diff
changeset
|
583 } |