# HG changeset patch # User lparsons # Date 1436802351 14400 # Node ID a51942c74761ad0fe9bd29fcba64b7ef42b5cf3b # Parent b9e7569a44384f9e519c8c8c57d6dc46cfe3eb83 planemo upload for repository https://github.com/lparsons/galaxy_tools/tree/master/tools/sam_stats commit 394834f8f34909961cfbf6252fefbdc63342d78d diff -r b9e7569a4438 -r a51942c74761 README.txt --- a/README.txt Fri Jun 27 15:36:01 2014 -0400 +++ b/README.txt Mon Jul 13 11:45:51 2015 -0400 @@ -1,12 +1,12 @@ -== sam-stats Galaxy Wrapper == +== ea-utils Galaxy Wrapper == -This is a Galaxy wrapper for the sam-stats tool from ea-utils suite. +This is a Galaxy wrapper for the sam-stats tools from the ea-utils suite. ** Installation ** Installation from a tool shed provides the necessary tool dependencies. -Otherwise, make sure sam-stats is in the path. +Otherwise, make sure fastq-join and sam-stats are in the path. Move the test data files to your galaxy root test-data. Move the xml files to a subdirectory of your tools directory and add lines in tool_conf.xml to point to them. Restart the Galaxy server. @@ -16,4 +16,3 @@ The ea-utils package and associated documentation can be found at: http://code.google.com/p/ea-utils/ The galaxy wrapper code was written by Lance Parsons (lparsons@princeton.edu), Lewis-Sigler Institute for Integrative Genomics, Princeton University. -The code is housed on BitBucket at: https://bitbucket.org/lance_parsons/ea_utils_galaxy_wrapper diff -r b9e7569a4438 -r a51942c74761 sam-stats.xml --- a/sam-stats.xml Fri Jun 27 15:36:01 2014 -0400 +++ b/sam-stats.xml Mon Jul 13 11:45:51 2015 -0400 @@ -1,9 +1,15 @@ - + - Compute statistics from SAM or BAM files + ea-utils - + + + + + + $samStats + ]]> + - + @@ -32,10 +40,6 @@ - - - - @@ -43,14 +47,14 @@ - + : mean, max, stdev, median, Q1 (25 percentile), Q3 reads : # of entries in the sam file, might not be # reads phred : phred scale used bsize : # reads used for qual stats @@ -62,14 +66,14 @@ ins rate : insert bases / total bases del rate : deleted bases / total bases pct mismatch : percent of reads that have mismatches - len <STATS> : read length stats, ignored if fixed-length - mapq <STATS> : stats for mapping qualities - insert <STATS> : stats for insert sizes - <CHR> : percentage of mapped bases per chr, followed by a signature + len : read length stats, ignored if fixed-length + mapq : stats for mapping qualities + insert : stats for insert sizes + : percentage of mapped bases per chr, followed by a signature Subsampled stats (1M reads max):: - base qual <STATS> : stats for base qualities + base qual : stats for base qualities A,T,C,G : base percentages Meaning of the per-chromosome signature: @@ -79,5 +83,27 @@ See http://code.google.com/p/ea-utils/wiki/SamStatsDetails for more information on each stat, how it's calculated and what it means. This tool uses the sam-stats program that is part of the ea-utils suite. See http://code.google.com/p/ea-utils/wiki/SamStats for details. +]]> + + + + @article{aronesty_comparison_2013, + title = {Comparison of {Sequencing} {Utility} {Programs}}, + volume = {7}, + issn = {18750362}, + url = {http://benthamopen.com/ABSTRACT/TOBIOIJ-7-1}, + doi = {10.2174/1875036201307010001}, + language = {en}, + number = {1}, + urldate = {2015-07-10}, + journal = {The Open Bioinformatics Journal}, + author = {Aronesty, Erik}, + month = jan, + year = {2013}, + pages = {1--8} + } + + +