Mercurial > repos > lz_hust > gatktools
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author | lz_hust |
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date | Sat, 01 Jun 2019 07:20:41 -0400 |
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--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/readme.rst Sat Jun 01 07:20:41 2019 -0400 @@ -0,0 +1,98 @@ +Galaxy wrapper for GATK2 +======================== + +This wrapper is copyright 2013 by Björn Grüning, Jim Johnson & the Galaxy Team. + +The Genome Analysis Toolkit or GATK is a software package developed at the +Broad Institute to analyse next-generation resequencing data. The toolkit offers +a wide variety of tools, with a primary focus on variant discovery and +genotyping as well as strong emphasis on data quality assurance. Its robust +architecture, powerful processing engine and high-performance computing features +make it capable of taking on projects of any size. + +http://www.broadinstitute.org/gatk +http://www.broadinstitute.org/gatk/about/citing-gatk + +GATK is Free for academics, and fee for commercial use. Please study the GATK licensing website: +http://www.broadinstitute.org/gatk/about/#licensing + + +Installation +============ + +The recommended installation is by means of the toolshed_. + +.. _toolshed: http://toolshed.g2.bx.psu.edu/view/iuc/gatk2 + +Galaxy should be able to install samtools dependencies automatically +for you. GATK2, and its new licence model, does not allow us to distribute the GATK binaries. +As a consequence you need to install GATK2 by your own, please see the GATK website for more information: + +http://www.broadinstitute.org/gatk/download + +Once you have installed GATK2, you need to edit the env.sh files that are installed together with the wrappers. +You must edit the GATK2_PATH environment variable in the file: + +<tool_dependency_dir>/environment_settings/GATK2_PATH/iuc/gatk2/<hash_string>/env.sh + +to point to the folder where you have installed GATK2. + +Optionally, you may also want to edit the GATK2_SITE_OPTIONS environment variable in the file: + +<tool_dependency_dir>/environment_settings/GATK2_SITE_OPTIONS/iuc/gatk2/<hash_string>/env.sh + +to deactivate the 'call home feature' of GATK with something like: + +GATK2_SITE_OPTIONS='-et NO_ET -K /data/gatk2_key_file' + +GATK2_SITE_OPTIONS can be also used to insert other specific options into every GATK2 wrapper +at runtime, without changing the actual wrapper. + +Read more about the "Phone Home" problem at: +http://www.broadinstitute.org/gatk/guide/article?id=1250 + +Optionally, you may also want to add some commands to be executed before GATK (e.g. to load modules) to the file: +:: + <tool_dependency_dir>/gatk2/default/env.sh + +Note that due to the manual nature of the GATK2 installation you will be getting the +following warnings in the Galaxy log (unless you specified the env.sh in the previous paragraph): +:: + Failed to resolve dependency on 'gatk2', ignoring. + +This is because the +:: + <requirement type="package">gatk2</requirement> +is specified but never resolved in the tool_dependencies.xml. It is safe to ignore. + +Finally, you should fill in additional information about your genomes and +annotations in the gatk2_picard_index.loc and gatk2_annotations.txt. +You can find them in the tool-data/ Galaxy directory. + +History +======= + +* v0.1 - Initial public release +* v2.8.0 - Bugfix release, increase version number to reflect the underlying GATK version + + +Licence (MIT) +============= + +Permission is hereby granted, free of charge, to any person obtaining a copy +of this software and associated documentation files (the "Software"), to deal +in the Software without restriction, including without limitation the rights +to use, copy, modify, merge, publish, distribute, sublicense, and/or sell +copies of the Software, and to permit persons to whom the Software is +furnished to do so, subject to the following conditions: + +The above copyright notice and this permission notice shall be included in +all copies or substantial portions of the Software. + +THE SOFTWARE IS PROVIDED "AS IS", WITHOUT WARRANTY OF ANY KIND, EXPRESS OR +IMPLIED, INCLUDING BUT NOT LIMITED TO THE WARRANTIES OF MERCHANTABILITY, +FITNESS FOR A PARTICULAR PURPOSE AND NONINFRINGEMENT. IN NO EVENT SHALL THE +AUTHORS OR COPYRIGHT HOLDERS BE LIABLE FOR ANY CLAIM, DAMAGES OR OTHER +LIABILITY, WHETHER IN AN ACTION OF CONTRACT, TORT OR OTHERWISE, ARISING FROM, +OUT OF OR IN CONNECTION WITH THE SOFTWARE OR THE USE OR OTHER DEALINGS IN +THE SOFTWARE.