Mercurial > repos > malbuquerque > factera
diff factera/factera.xml @ 18:c793d9c3f26b draft
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author | malbuquerque |
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date | Fri, 16 Jan 2015 17:40:04 -0500 |
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--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/factera/factera.xml Fri Jan 16 17:40:04 2015 -0500 @@ -0,0 +1,60 @@ +<tool id="factera" name="FACTERA" version="1.4.3"> + + <description>Fusion And Chromosomal Translocation Enumeration and Recovery Algorithm</description> + + <requirements> + <requirement type="package" version="0.1.19">samtools</requirement> + <requirement type="package" version="2.2.26+">blast+</requirement> + <requirement type="package" version="310">ucsc_user_apps</requirement> + <requirement type="package" version="5.18.1">perl</requirement> + <requirement type="package" version="1.4.3">factera</requirement> + <requirement type="package" version="3.0608">perl_descriptive_statistics</requirement> + <requirement type="set_environment">FACTERA_DIR</requirement> + </requirements> + + <command> + <!-- Run commands using Galaxy perl --> + #if $cond.additionalOpt == "no" + perl \$FACTERA_DIR/factera-v1.4.3.pl -o -F ./ $sortedBam $exonBed $genome2Bit; + #else + perl \$FACTERA_DIR/factera-v1.4.3.pl -r -F $cond.r -m $cond.m -x $cond.x -s $cond.s -f $cond.f -S $cond.S + -k $cond.k -c $cond.c -b $cond.b -p $cond.p -a $cond.a -o ./ $sortedBam $exonBed $genome2Bit; + #end if + + <!-- Keep only the fusion file --> + if [ -f "*fusions.txt" ]; then cat *fusions.txt > $outfile ; else echo "No Fusions" > $outfile ; fi + </command> + + <inputs> + <param format="bam" name="sortedBam" type="data" label="Alignment in BAM format" /> + <param format="bed" name="exonBed" type="data" label="Genomic coordinates with gene/exon names in fourth column" /> + <param format="twobit" name="genome2Bit" type="data" label="Two bit reference sequence" /> + <conditional name="cond"> + <param type="select" name="additionalOpt" label="Additional Options"> + <option value="yes">Yes</option> + <option value="no" selected="True">No</option> + </param> + <when value="yes"> + <!-- Names follow command line flags in factera.pl --> + <param type="integer" name="r" label="Minimum number of breakpoint-scanning reads required for output" value="5"/> + <param type="integer" name="m" label="Minimum number of discordant reads required for each candidate fusion" value="2"/> + <param type="integer" name="x" label="Maximum number of breakpoints to examine for each pair of genomic regions" value="5"/> + <param type="integer" name="s" label="Minimum number of reads with the same breakpoint" value="1"/> + <param type="float" name="f" min="0" max="1" label="Minimum fraction of read bases required for alignment to fusion template" value=".9"/> + <param type="float" name="S" min="0" max="1" label="Minimum similarity required for read to match fusion template" value=".95"/> + <param type="integer" name="k" label="K-mer size (number of bases) for fragment comparison" value="10"/> + <param type="integer" name="c" label="Minimum size of soft-clipped region to consider" value="16"/> + <param type="integer" name="b" label="Number of bases flanking breakpoint for fusion template" value="500"/> + <param type="integer" name="p" label="Number of threads for blastn search" value="4"/> + <param type="integer" name="a" label="Number of bases flanking breakpoint to provide in output" value="50"/> + </when> + </conditional> + </inputs> + + <outputs> + <data format="tabular" name="outfile" /> + </outputs> + + <help>This tool does Factera</help> + +</tool>