Mercurial > repos > malex > ribopicker
diff ribopicker.xml @ 0:75ca0166e969 draft
Uploaded
author | malex |
---|---|
date | Tue, 16 Oct 2012 19:42:32 -0400 |
parents | |
children |
line wrap: on
line diff
--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/ribopicker.xml Tue Oct 16 19:42:32 2012 -0400 @@ -0,0 +1,97 @@ +<tool id="ribopicker" name="riboPicker" version="1.0.0"> + <description>Easy identification and removal of rRNA-like sequences.</description> + <version_command>ribopicker.pl -version | awk '{print $3}'</version_command> + <command interpreter="bash">ribopicker.sh + -id 'galaxy' + #if $identity_threshold: + -i $identity_threshold + #end if + #if $coverage_threshold: + -c $coverage_threshold + #end if + #if $length_threshold: + -l $length_threshold + #end if + #if $read_chunk_size: + -S $read_chunk_size + #end if + #if $z_best_value: + -z $z_best_value + #end if + #if $align_score_threshold: + -T $align_score_threshold + #end if + -f $input + </command> + <inputs> + <param name="input" type="data" format="fastq,fasta" label="from" + help="Input file in FASTA or FASTQ format that contains the query + sequences."/> + <param name="dbs" type="select" label="Reference Database"> + <options from_data_table="ribopicker_dbs"> + <filter type="sort_by" column="2" /> + <validator type="no_options" message="No reference databases are available" /> + </options> + </param> + <param name="identity_threshold" type="integer" value="" size="3" + label="Alignment Identity Threshold" optional="True" help="Alignment + identity threshold in percentage (integer from 1-100 without %) used to + define matching sequences as similar. The identity is calculated for the + part of the query sequence that is aligned to a reference sequence. For + example, a query sequence of 100 bp that aligns to a reference sequence + over the first 50 bp with 40 matching positions has an identity value of + 80%." > + <validator type="in_range" message="(1-100)" min="1" max="100"/> + </param> + <param name="coverage_threshold" type="integer" value="" size="3" + label="Alignment Coverage Threshold" optional="True" help="Alignment + coverage threshold in percentage (integer from 1-100 without %) used to + define matching sequences as similar. The coverage is calculated for the + part of the query sequence that is aligned to a reference sequence. For + example, a query sequence of 100 bp that aligns to a reference sequence + over the first 50 bp with 40 matching positions has an coverage value of + 50%." > + <validator type="in_range" message="(1-100)" min="1" max="100"/> + </param> + <param name="length_threshold" type="integer" value="" size="5" + label="Alignment Length Threshold" optional="True" help="Alignment + length threshold used to define matching sequences as similar. For + example, a query sequence of 100 bp that aligns to a reference sequence + over the first 50 bp with 40 matching positions has an alignment length + of 50." > + <validator type="in_range" message="(1-infinity)" min="1" max="inf"/> + </param> + <param name="read_chunk_size" type="integer" value="" size="9" + label="Chunk size of reads in bp for BWA-SW" optional="True" help="Chunk + size of reads in bp as used by BWA-SW (default: 10000000)" > + <validator type="in_range" message="(1-infinity)" min="1" max="inf"/> + </param> + <param name="z_best_value" type="integer" value="" size="5" + label="Z-best value for BWA-SW" optional="True" help="Z-best value as + used by BWA-SW (default: 1)"> + <validator type="in_range" message="(1-infinity)" min="1" max="inf"/> + </param> + <param name="align_score_threshold" type="integer" value="" size="5" + label="Alignment score threshold for BWA-SW" optional="True" + help="Alignment score threshold as used by BWA-SW (default: 30)" > + <validator type="in_range" message="(1-infinity)" min="1" max="inf"/> + </param> + </inputs> + <outputs> + <data name="rrna" format="fasta" from_work_dir="galaxy_rrna.txt" label="rRNA" /> + <data name="nonrrna" format="fasta" from_work_dir="galaxy_nonrrna.txt" label="Non-rRNA" /> + </outputs> + <!-- + Usage: + ribopicker [options] -f <file> -dbs <list> ... + Options: + -T <integer> + Alignment score threshold as used by BWA-SW (default: 30). +--> +<help> +**What it does** + +The riboPicker tool can be used to automatically identify and efficiently +remove rRNA-like sequences from metatranscriptomic and metagenomic datasets. +</help> +</tool>