Mercurial > repos > matces > carpet_toolsuite
annotate carpet-src-1/tools/CARPET/PeakPeaker2.pl @ 1:78770028dcf1 default tip
Migrated tool version 1.0.1 from old tool shed archive to new tool shed repository
author | matces |
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date | Tue, 07 Jun 2011 16:59:33 -0400 |
parents | cdd489d98766 |
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1 #! /usr/bin/perl |
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2 |
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3 # Copyright 2009 Matteo Cesaroni, Lucilla Luzi |
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4 # |
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5 # This program is free software; ; you can redistribute it and/or modify |
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6 # it under the terms of the GNU Lesser General Public License as published by |
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7 # the Free Software Foundation; either version 3 of the License, or (at your |
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8 # option) any later version. |
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9 # |
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10 # This program is distributed in the hope that it will be useful, |
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11 # but WITHOUT ANY WARRANTY; without even the implied warranty of |
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12 # MERCHANTABILITY or FITNESS FOR A PARTICULAR PURPOSE. See the |
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13 # GNU General Public License for more details. |
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14 |
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15 |
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16 use Getopt::Long; |
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17 |
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18 GetOptions ( |
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19 "help" => \$OPT{help}, |
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20 "in=s" => \$OPT{fname}, |
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21 "perc=s" => \$OPT{value}, |
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22 "fc=s" => \$OPT{fc_value}, |
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23 "log=s" => \$OPT{valore_log}, |
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24 "t=s" => \$OPT{type_peak}, |
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25 "num=s" => \$OPT{num_probe}, |
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26 "dist=s" => \$OPT{dist_max}, |
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27 "dist_peaks=s" => \$OPT{dist_max_peaks}, |
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28 "w=s"=> \$OPT{s_windows}, |
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29 "col3=s" => \$OPT{col3}, |
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30 "f_pv=s" => \$OPT{file_pv}, |
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31 "out=s"=> \$OPT{out} |
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32 |
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33 )|| printusage(); |
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34 |
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35 # opzioni da linea di comando |
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36 my $value=$OPT{value}; |
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37 my $fc_value=$OPT{fc_value}; |
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38 my $valore_log=$OPT{valore_log}; |
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39 my $type_peak=$OPT{type_peak}; |
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40 my $num_probe=$OPT{num_probe}; |
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41 my $dist_max=$OPT{dist_max}; |
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42 my $dist_max_peaks=$OPT{dist_max_peaks}; |
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43 my $fname=$OPT{fname}; |
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44 my $help=$OPT{help}; |
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45 my $s_windows=$OPT{s_windows}; |
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46 my $col3=$OPT{col3}; |
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47 my $outfile=$OPT{out}; |
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48 my $outfile_pv=$OPT{file_pv}, |
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49 |
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50 # "usage" se c'e' un help |
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51 $help and printusage(); |
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52 |
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53 # "usage" se non ci sono opzioni |
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54 if (!$s_windows || !$fname || (!$value && !$fc_value) || !$type_peak || !$num_probe || !$dist_max || (($type_peak eq "p") && !$valore_log)){ |
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55 &printusage() |
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56 }; |
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57 |
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58 |
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59 qx {sort -k 1,1 -k 4,4n $fname >$fname.sortato}; |
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60 |
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61 |
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62 open(FILE, "<$fname.sortato") or die "Cannot find file $fname.sortato\n"; |
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63 open(pvalue, ">$outfile_pv") or die "Cannot open file $outfile_pv: $!\n"; |
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64 #open(buonitutti, "> buoni_$fname") or die "Cannot find file $fname: $!\n"; |
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65 #loop th rought line-by-line until the end of the file and then push each line into an empty array, called @array# |
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66 print pvalue "#p-value track ($value) \n"; |
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67 |
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68 |
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69 @array= (); |
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70 $conto_tutti_sopra=0; |
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71 $conto_tutti=0; |
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72 while ($line = <FILE>){ |
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73 chomp ($line); |
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74 if ($line=~/track/g){next;} |
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75 if ($line=~/#/g){next;} |
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76 if ($line=~/^\s+$/g){next;} |
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77 push (@array,$line); |
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78 @value_perc=split("\t",$line); |
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79 push (@percentile,$value_perc[5]); |
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80 if ($value_perc[5]>=$fc_value){ |
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81 $conto_tutti_sopra++; |
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82 } |
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83 elsif ($value_perc[5]<$fc_value){ |
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84 $conto_tutti++; |
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85 } |
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86 } |
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87 |
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88 close (FILE); |
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89 |
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90 if (!$fc_value){ |
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91 @perc_ordine=sort(@percentile); |
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92 $position=(($value*($#perc_ordine+1))-1); |
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93 $valore_percentile=$perc_ordine[$position]; |
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94 #print "il percentile è $valore_percentile\n"; |
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95 #print "il max è $perc_ordine[$#perc_ordine]\n"; |
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96 #print "il min è $perc_ordine[1]\n"; |
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97 $probabilita=1-$value; |
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98 } |
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99 else { |
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100 $valore_percentile=$fc_value; |
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101 $tuttitutti=($conto_tutti+$conto_tutti_sopra); |
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102 #print"il numero totaledi probe è $tuttitutti\n"; |
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103 #print"il numero totale di probe sopra è $conto_tutti_sopra\n"; |
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104 $probabilita=$conto_tutti_sopra/($conto_tutti+$conto_tutti_sopra); |
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105 #print "la proba= $probabilita\n"; |
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106 } |
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107 |
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108 #print buonitutti"il percentile e $valore_percentile\n"; |
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109 |
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110 |
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111 |
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112 ($one_ref,$two_ref)=&probe_cutoff(@array); |
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113 |
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114 if ($type_peak eq "p"){ |
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115 @forse_niente=&peak(@$one_ref); |
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116 } |
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117 elsif ($type_peak eq "s"){ |
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118 @forse_niente=&peak(@$two_ref); |
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119 } |
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120 |
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121 &double_peak(@forse_niente); |
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122 |
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123 # |
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124 # End process |
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125 # |
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126 close OUTFILE; |
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127 unlink "$fname.sortato"; |
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128 exit 0; |
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129 |
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130 ################################################################################################ |
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131 ######################## SUBROUTINE ############################### |
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132 ################################################################################################ |
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133 |
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134 sub probe_cutoff |
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135 { |
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136 |
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137 $c=-6; |
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138 |
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139 foreach $linea(@array){ |
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140 @subarray1 = split("\t",$linea); |
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141 $inizio=(((($subarray1[4]-$subarray1[3])/2)+$subarray1[3])-($s_windows/2)); #per modificare la windows cambia il 500 e il 1000 |
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142 $fine=$inizio+$s_windows; #windows 500 -->250 e 500 windows 1000 --> 500 e 1000 |
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143 $counter=0; |
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144 $counter_value=0; |
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145 |
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146 if($subarray1[5]>=$valore_percentile){ |
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147 push (@array_probe,[@subarray1]); |
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148 print buonitutti "$linea\n"; |
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149 } |
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150 |
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151 for($i=$c;$i<=$#array;$i++) { |
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152 if ($i<0){ |
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153 next; |
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154 } |
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155 @subarray = split("\t",$array[$i]); |
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156 if (($subarray[3]>=$inizio)&&($subarray[3]<$fine)&&("$subarray[0]" eq "$subarray1[0]")){ |
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157 $counter++; |
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158 if ($subarray[5]>= $valore_percentile){ |
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159 $counter_value++; |
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160 } |
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161 } |
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162 if (($subarray[3]>$fine)||("$subarray[0]" ne "$subarray1[0]")){ |
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163 $cazzo=$subarray[3]; |
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164 last; |
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165 } |
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166 |
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167 } |
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168 $c++; |
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169 if ($counter !=0){ |
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170 $chi_sq = (((($counter_value - ($probabilita*$counter))**2)/($probabilita*$counter))+(((($counter-$counter_value)-((1-$probabilita)*$counter))**2)/((1-$probabilita)*$counter))); |
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171 } |
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172 else{ |
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173 $chi_sq = NA; |
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174 } |
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175 |
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176 use Statistics::Distributions; |
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177 $chisprob=Statistics::Distributions::chisqrprob (1,$chi_sq); |
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178 if ($chisprob == 0){ |
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179 $log_10=100; |
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180 } |
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181 else { |
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182 $log_10 = -log($chisprob)/log(10); |
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183 } |
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184 |
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185 print pvalue "$subarray1[0]\t$subarray1[1]\tpv_$subarray1[2]\t$subarray1[3]\t$subarray1[4]\t$log_10\t$subarray1[6]\t$subarray1[7]\t$subarray1[5]\n"; |
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186 |
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187 if ($log_10>=$valore_log){ |
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188 #print"$subarray1[3],$inizio,$fine,$counter,$counter_value,$chi_sq,$chisprob,$log_10\n"; |
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189 @proviamo=($subarray1[0],$subarray1[1],$subarray1[2],$inizio,$fine,$log_10,$subarray1[6],$subarray1[7],$subarray1[5]); |
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190 push (@matrix_pvalue,[@proviamo]); |
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191 |
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192 } |
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193 } |
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194 @result_table=@matrix_pvalue; |
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195 @result_table2=@array_probe; |
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196 return(\@result_table,\@result_table2); |
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197 |
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198 } |
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199 |
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200 |
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201 |
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202 |
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203 |
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204 ################################################################################################ |
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205 |
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206 |
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207 |
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208 sub peak |
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209 { |
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210 @matrix_value=@_; |
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211 |
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212 $stop=$#matrix_value; |
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213 |
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214 for ($cio=0; $cio<=$stop;$cio++) { |
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215 @log_ratio=(); |
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216 @chilosa=(); |
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217 $inizio=$matrix_value[$cio][3]; |
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218 $somma=0; |
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219 $media=0; |
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220 $sommachilo=0; |
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221 $mediachilo=0; |
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222 $diviso=0; |
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223 push(@log_ratio,$matrix_value[$cio][5]); |
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224 if ($matrix_value[$cio][8]>=$valore_percentile){ |
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225 push(@chilosa,$matrix_value[$cio][8]); |
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226 } |
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227 for ($j=0; $j<=$stop;$j++){ |
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228 $distanza=($matrix_value[$cio+1][3]-$matrix_value[$cio][4]); |
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229 if (($distanza > $dist_max) || ($cio==$stop)||(!("$matrix_value[$cio+1][0]" eq "$matrix_value[$cio][0]"))){ |
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230 $j=$stop; |
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231 } |
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232 elsif (($distanza <= $dist_max)&&("$matrix_value[$cio+1][0]" eq "$matrix_value[$cio][0]")){ |
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233 $cio++; |
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234 push(@log_ratio,$matrix_value[$cio][5]); |
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235 $fine=$matrix_value[$cio][4]; |
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236 $inizio3=$inizio; |
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237 $j++; |
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238 if ($matrix_value[$cio][8]>=$valore_percentile){ |
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239 push(@chilosa,$matrix_value[$cio][8]); |
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240 } |
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241 } |
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242 |
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243 |
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244 } |
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245 $numeroprobes=($#chilosa+1); |
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246 if ((($#log_ratio+1)>=$num_probe) && (($type_peak eq "s") ||(($#chilosa+1)>=$num_probe))){ |
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247 foreach $n (@log_ratio) { |
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248 $somma+=$n; |
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249 } |
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250 if($type_peak eq "s"){ |
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251 @chilosa=@log_ratio; |
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252 } |
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253 foreach $nchilo (@chilosa) { |
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254 $sommachilo+=$nchilo; |
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255 } |
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256 $diviso=$#log_ratio+1; |
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257 $media = $somma/(@log_ratio); |
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258 $mediachilo=$sommachilo/(@chilosa); |
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259 #$somma_media_chilo=((sqrt($#chilosa+1))+$mediachilo); |
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260 #$moltipli=(($#log_ratio+1)*$media); |
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261 #$somma_media=((sqrt($#log_ratio+1))+$media); |
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262 if ($type_peak eq "p"){ |
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263 $inizio3=int($inizio3+(($s_windows/2)-25)); |
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Migrated tool version 1.0.0 from old tool shed archive to new tool shed repository
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264 $fine=int($fine-(($s_windows/2)-25)); |
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Migrated tool version 1.0.0 from old tool shed archive to new tool shed repository
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265 } |
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Migrated tool version 1.0.0 from old tool shed archive to new tool shed repository
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266 @proviamo_double=($matrix_value[$cio][0],$matrix_value[$cio][1],$col3,$inizio3,$fine,$media,$matrix_value[$cio][6],$matrix_value[$cio][7],$mediachilo,$diviso); |
cdd489d98766
Migrated tool version 1.0.0 from old tool shed archive to new tool shed repository
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267 push (@matrix_for_double,[@proviamo_double]); |
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Migrated tool version 1.0.0 from old tool shed archive to new tool shed repository
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268 |
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Migrated tool version 1.0.0 from old tool shed archive to new tool shed repository
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269 } |
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Migrated tool version 1.0.0 from old tool shed archive to new tool shed repository
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270 } |
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Migrated tool version 1.0.0 from old tool shed archive to new tool shed repository
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271 |
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Migrated tool version 1.0.0 from old tool shed archive to new tool shed repository
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272 @result_table=@matrix_for_double; |
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Migrated tool version 1.0.0 from old tool shed archive to new tool shed repository
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273 return(@result_table); |
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Migrated tool version 1.0.0 from old tool shed archive to new tool shed repository
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274 } |
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Migrated tool version 1.0.0 from old tool shed archive to new tool shed repository
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275 |
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Migrated tool version 1.0.0 from old tool shed archive to new tool shed repository
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276 |
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Migrated tool version 1.0.0 from old tool shed archive to new tool shed repository
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277 ################################################################################################ |
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278 |
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Migrated tool version 1.0.0 from old tool shed archive to new tool shed repository
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279 |
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Migrated tool version 1.0.0 from old tool shed archive to new tool shed repository
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280 |
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Migrated tool version 1.0.0 from old tool shed archive to new tool shed repository
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281 sub double_peak |
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Migrated tool version 1.0.0 from old tool shed archive to new tool shed repository
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282 { |
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283 @matrix_value=@_; |
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284 |
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Migrated tool version 1.0.0 from old tool shed archive to new tool shed repository
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285 $stop=$#matrix_value; |
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Migrated tool version 1.0.0 from old tool shed archive to new tool shed repository
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286 $i=0; |
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Migrated tool version 1.0.0 from old tool shed archive to new tool shed repository
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287 $j=0; |
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Migrated tool version 1.0.0 from old tool shed archive to new tool shed repository
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288 #print $stop; |
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289 |
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Migrated tool version 1.0.0 from old tool shed archive to new tool shed repository
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290 # |
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291 # Print output file |
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292 # |
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293 if ($outfile){ |
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Migrated tool version 1.0.0 from old tool shed archive to new tool shed repository
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294 open (OUTFILE, ">$outfile"); |
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Migrated tool version 1.0.0 from old tool shed archive to new tool shed repository
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|
295 } |
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Migrated tool version 1.0.0 from old tool shed archive to new tool shed repository
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296 |
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Migrated tool version 1.0.0 from old tool shed archive to new tool shed repository
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297 # |
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Migrated tool version 1.0.0 from old tool shed archive to new tool shed repository
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298 # print File Header |
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299 # |
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300 my $header=<<e0c6654; |
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301 \# infile: $fname |
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302 \# percentile value: $value=$valore_percentile |
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303 \# fold change: $fc_value |
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Migrated tool version 1.0.0 from old tool shed archive to new tool shed repository
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304 \# type of analysis: $type_peak |
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Migrated tool version 1.0.0 from old tool shed archive to new tool shed repository
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305 \# log(pval analysis): $valore_log |
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Migrated tool version 1.0.0 from old tool shed archive to new tool shed repository
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306 \# num (probe defining a peak): $num_probe |
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Migrated tool version 1.0.0 from old tool shed archive to new tool shed repository
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307 \# dist: $dist_max |
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Migrated tool version 1.0.0 from old tool shed archive to new tool shed repository
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308 \# window length: $s_windows |
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Migrated tool version 1.0.0 from old tool shed archive to new tool shed repository
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309 track name=$col3 description="peaks find" visibility=2 |
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Migrated tool version 1.0.0 from old tool shed archive to new tool shed repository
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310 e0c6654 |
cdd489d98766
Migrated tool version 1.0.0 from old tool shed archive to new tool shed repository
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|
311 |
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Migrated tool version 1.0.0 from old tool shed archive to new tool shed repository
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312 if ($outfile){ |
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Migrated tool version 1.0.0 from old tool shed archive to new tool shed repository
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313 print OUTFILE "$header"; |
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Migrated tool version 1.0.0 from old tool shed archive to new tool shed repository
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314 }else{ |
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Migrated tool version 1.0.0 from old tool shed archive to new tool shed repository
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315 print "$header"; |
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Migrated tool version 1.0.0 from old tool shed archive to new tool shed repository
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|
316 } |
cdd489d98766
Migrated tool version 1.0.0 from old tool shed archive to new tool shed repository
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317 if ($type_peak eq "p"){ |
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Migrated tool version 1.0.0 from old tool shed archive to new tool shed repository
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318 print "perc value=$value=$valore_percentile, #probes=$num_probe (dist=$dist_max), window=$s_windows, type analisys =p-value (log=$valore_log), dist peaks=$dist_max_peaks"; |
cdd489d98766
Migrated tool version 1.0.0 from old tool shed archive to new tool shed repository
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319 } |
cdd489d98766
Migrated tool version 1.0.0 from old tool shed archive to new tool shed repository
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320 if ($type_peak eq "s"){ |
cdd489d98766
Migrated tool version 1.0.0 from old tool shed archive to new tool shed repository
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321 print "perc value=$value=$valore_percentile, #probes=$num_probe (dist=$dist_max), window=$s_windows, type analisys=score, dist peaks=$dist_max_peaks"; |
cdd489d98766
Migrated tool version 1.0.0 from old tool shed archive to new tool shed repository
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322 } |
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Migrated tool version 1.0.0 from old tool shed archive to new tool shed repository
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323 for ($i=0; $i<=$stop;$i++) { |
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Migrated tool version 1.0.0 from old tool shed archive to new tool shed repository
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324 @log_ratio=(); |
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Migrated tool version 1.0.0 from old tool shed archive to new tool shed repository
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325 @log_ratio2=(); |
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Migrated tool version 1.0.0 from old tool shed archive to new tool shed repository
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326 @diviso2=(); |
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Migrated tool version 1.0.0 from old tool shed archive to new tool shed repository
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327 $inizio=$matrix_value[$i][3]; |
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Migrated tool version 1.0.0 from old tool shed archive to new tool shed repository
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328 $somma=0; |
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Migrated tool version 1.0.0 from old tool shed archive to new tool shed repository
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329 $somma2=0; |
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Migrated tool version 1.0.0 from old tool shed archive to new tool shed repository
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330 $media=0; |
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Migrated tool version 1.0.0 from old tool shed archive to new tool shed repository
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331 $media2=0; |
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Migrated tool version 1.0.0 from old tool shed archive to new tool shed repository
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332 $diviso2=0; |
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Migrated tool version 1.0.0 from old tool shed archive to new tool shed repository
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333 push(@log_ratio,$matrix_value[$i][5]); |
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Migrated tool version 1.0.0 from old tool shed archive to new tool shed repository
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334 push(@log_ratio2,$matrix_value[$i][8]); |
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Migrated tool version 1.0.0 from old tool shed archive to new tool shed repository
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335 push(@diviso2,$matrix_value[$i][9]); |
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Migrated tool version 1.0.0 from old tool shed archive to new tool shed repository
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336 #print "giro $i\n"; |
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Migrated tool version 1.0.0 from old tool shed archive to new tool shed repository
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337 for ($j=0; $j<=$stop;$j++){ |
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Migrated tool version 1.0.0 from old tool shed archive to new tool shed repository
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338 # if ("$matrix_value[$i+1][0]" eq "$matrix_value[$i][0]"){ |
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Migrated tool version 1.0.0 from old tool shed archive to new tool shed repository
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339 $distanza=($matrix_value[$i+1][3]-$matrix_value[$i][4]); |
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Migrated tool version 1.0.0 from old tool shed archive to new tool shed repository
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340 #print "distanza fra i due $distanza\n"; |
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Migrated tool version 1.0.0 from old tool shed archive to new tool shed repository
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341 #exit; |
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Migrated tool version 1.0.0 from old tool shed archive to new tool shed repository
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342 if (($distanza > $dist_max_peaks) || ($i==$stop)||(!("$matrix_value[$i+1][0]" eq "$matrix_value[$i][0]"))){ |
cdd489d98766
Migrated tool version 1.0.0 from old tool shed archive to new tool shed repository
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343 $j=$stop; |
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Migrated tool version 1.0.0 from old tool shed archive to new tool shed repository
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344 $fine=$matrix_value[$i][4]; |
cdd489d98766
Migrated tool version 1.0.0 from old tool shed archive to new tool shed repository
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|
345 } |
cdd489d98766
Migrated tool version 1.0.0 from old tool shed archive to new tool shed repository
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346 elsif (($distanza <= $dist_max_peaks)&&("$matrix_value[$i+1][0]" eq "$matrix_value[$i][0]")){ |
cdd489d98766
Migrated tool version 1.0.0 from old tool shed archive to new tool shed repository
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347 $i++; |
cdd489d98766
Migrated tool version 1.0.0 from old tool shed archive to new tool shed repository
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348 push(@log_ratio,$matrix_value[$i][5]); |
cdd489d98766
Migrated tool version 1.0.0 from old tool shed archive to new tool shed repository
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349 push(@log_ratio2,$matrix_value[$i][8]); |
cdd489d98766
Migrated tool version 1.0.0 from old tool shed archive to new tool shed repository
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350 push(@diviso2,$matrix_value[$i][9]); |
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Migrated tool version 1.0.0 from old tool shed archive to new tool shed repository
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351 $fine=$matrix_value[$i][4]; |
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Migrated tool version 1.0.0 from old tool shed archive to new tool shed repository
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352 $j++; |
cdd489d98766
Migrated tool version 1.0.0 from old tool shed archive to new tool shed repository
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|
353 } |
cdd489d98766
Migrated tool version 1.0.0 from old tool shed archive to new tool shed repository
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354 |
cdd489d98766
Migrated tool version 1.0.0 from old tool shed archive to new tool shed repository
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355 # } |
cdd489d98766
Migrated tool version 1.0.0 from old tool shed archive to new tool shed repository
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|
356 } |
cdd489d98766
Migrated tool version 1.0.0 from old tool shed archive to new tool shed repository
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357 foreach $n (@log_ratio) { |
cdd489d98766
Migrated tool version 1.0.0 from old tool shed archive to new tool shed repository
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358 $somma+=$n; |
cdd489d98766
Migrated tool version 1.0.0 from old tool shed archive to new tool shed repository
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diff
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|
359 } |
cdd489d98766
Migrated tool version 1.0.0 from old tool shed archive to new tool shed repository
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360 foreach $n1 (@log_ratio2) { |
cdd489d98766
Migrated tool version 1.0.0 from old tool shed archive to new tool shed repository
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changeset
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361 $somma2+=$n1; |
cdd489d98766
Migrated tool version 1.0.0 from old tool shed archive to new tool shed repository
matces
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diff
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|
362 } |
cdd489d98766
Migrated tool version 1.0.0 from old tool shed archive to new tool shed repository
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changeset
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363 foreach $n2 (@diviso2) { |
cdd489d98766
Migrated tool version 1.0.0 from old tool shed archive to new tool shed repository
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changeset
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364 $diviso2+=$n2; |
cdd489d98766
Migrated tool version 1.0.0 from old tool shed archive to new tool shed repository
matces
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diff
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|
365 } |
cdd489d98766
Migrated tool version 1.0.0 from old tool shed archive to new tool shed repository
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366 $media = $somma/(@log_ratio); |
cdd489d98766
Migrated tool version 1.0.0 from old tool shed archive to new tool shed repository
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|
367 $media2 = $somma2/(@log_ratio2); |
cdd489d98766
Migrated tool version 1.0.0 from old tool shed archive to new tool shed repository
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368 $somma_media=(sqrt($diviso2)+$media); |
cdd489d98766
Migrated tool version 1.0.0 from old tool shed archive to new tool shed repository
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369 $somma_score=(sqrt($diviso2)+$media2); |
cdd489d98766
Migrated tool version 1.0.0 from old tool shed archive to new tool shed repository
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370 $somma_media = sprintf "%.2f", $somma_media; |
cdd489d98766
Migrated tool version 1.0.0 from old tool shed archive to new tool shed repository
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371 $somma_score = sprintf "%.2f", $somma_score; |
cdd489d98766
Migrated tool version 1.0.0 from old tool shed archive to new tool shed repository
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372 #$somma_score = $somma_score.$i |
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Migrated tool version 1.0.0 from old tool shed archive to new tool shed repository
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373 #$somma_media_chilo=((sqrt($#chilosa+1))+$mediachilo); |
cdd489d98766
Migrated tool version 1.0.0 from old tool shed archive to new tool shed repository
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374 if ($outfile){ |
cdd489d98766
Migrated tool version 1.0.0 from old tool shed archive to new tool shed repository
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375 print OUTFILE "$matrix_value[$i][0]\t$matrix_value[$i][1]\t$matrix_value[$i][2]\t$inizio\t$fine\t$somma_media\t$matrix_value[$i][6]\t$matrix_value[$i][7]\t$somma_score$i\n"; |
cdd489d98766
Migrated tool version 1.0.0 from old tool shed archive to new tool shed repository
matces
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diff
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376 }else{ |
cdd489d98766
Migrated tool version 1.0.0 from old tool shed archive to new tool shed repository
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377 print "$matrix_value[$i][0]\t$matrix_value[$i][1]\t$matrix_value[$i][2]\t$inizio\t$fine\t$somma_media\t$matrix_value[$i][6]\t$matrix_value[$i][7]\t$somma_score$i\n"; |
cdd489d98766
Migrated tool version 1.0.0 from old tool shed archive to new tool shed repository
matces
parents:
diff
changeset
|
378 } |
cdd489d98766
Migrated tool version 1.0.0 from old tool shed archive to new tool shed repository
matces
parents:
diff
changeset
|
379 |
cdd489d98766
Migrated tool version 1.0.0 from old tool shed archive to new tool shed repository
matces
parents:
diff
changeset
|
380 |
cdd489d98766
Migrated tool version 1.0.0 from old tool shed archive to new tool shed repository
matces
parents:
diff
changeset
|
381 } |
cdd489d98766
Migrated tool version 1.0.0 from old tool shed archive to new tool shed repository
matces
parents:
diff
changeset
|
382 } |
cdd489d98766
Migrated tool version 1.0.0 from old tool shed archive to new tool shed repository
matces
parents:
diff
changeset
|
383 |
cdd489d98766
Migrated tool version 1.0.0 from old tool shed archive to new tool shed repository
matces
parents:
diff
changeset
|
384 #################################################################################################################################### |
cdd489d98766
Migrated tool version 1.0.0 from old tool shed archive to new tool shed repository
matces
parents:
diff
changeset
|
385 |
cdd489d98766
Migrated tool version 1.0.0 from old tool shed archive to new tool shed repository
matces
parents:
diff
changeset
|
386 sub printusage { |
cdd489d98766
Migrated tool version 1.0.0 from old tool shed archive to new tool shed repository
matces
parents:
diff
changeset
|
387 |
cdd489d98766
Migrated tool version 1.0.0 from old tool shed archive to new tool shed repository
matces
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diff
changeset
|
388 print<<eoc22334; |
cdd489d98766
Migrated tool version 1.0.0 from old tool shed archive to new tool shed repository
matces
parents:
diff
changeset
|
389 |
cdd489d98766
Migrated tool version 1.0.0 from old tool shed archive to new tool shed repository
matces
parents:
diff
changeset
|
390 |
cdd489d98766
Migrated tool version 1.0.0 from old tool shed archive to new tool shed repository
matces
parents:
diff
changeset
|
391 |
cdd489d98766
Migrated tool version 1.0.0 from old tool shed archive to new tool shed repository
matces
parents:
diff
changeset
|
392 *************************************************** |
cdd489d98766
Migrated tool version 1.0.0 from old tool shed archive to new tool shed repository
matces
parents:
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changeset
|
393 :: N i m b l e G e n C h i p A n a l y s i s :: |
cdd489d98766
Migrated tool version 1.0.0 from old tool shed archive to new tool shed repository
matces
parents:
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changeset
|
394 *************************************************** |
cdd489d98766
Migrated tool version 1.0.0 from old tool shed archive to new tool shed repository
matces
parents:
diff
changeset
|
395 |
cdd489d98766
Migrated tool version 1.0.0 from old tool shed archive to new tool shed repository
matces
parents:
diff
changeset
|
396 |
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397 USAGE SUMMARY |
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398 --------------------------------------------------------------------------------- |
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399 This program utilizes NimbleGen ratio files in gff format as INPUT FILE and |
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400 provides a table of the computed picks in the same gff file format. |
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401 |
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402 |
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403 |
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404 --in [input filename] |
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405 --perc [percentile value, it is used to calculate the threshold rate based |
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406 on dataset distribution to filter out background ratio; i.e. 0.99] |
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407 OR |
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408 --fc [fold change value, it is used as fixed threshold to filter out |
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409 background ratio, i.e. 2] |
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410 --t [type of analysis; p, performs peaks determination based on p-value inference; |
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411 s, performs peaks determination based on a scoring system] |
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412 --log (required only for p-value based analysis) |
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413 [log2(p-value), cutoff integer to be used to identify a significant peak; i.e. 5] |
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414 --num [minimal number of consecutive probes used to define a peak; i.e. 3] |
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415 --dist [greatest nucleotide distance between two probes or between two peaks that |
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416 allow to consider the signals as belonging to the same peak] |
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417 --w [window length] |
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418 |
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419 --out [output filename (optional)] |
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420 ----------------------------------------------------------------------------------------- |
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421 EXAMPLES: |
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422 |
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423 perl program.pl --in 75340_ratio.gff --perc 0.98 --t s --num 3 --dist 250 --w 500 |
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424 OR |
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425 perl program.pl --in 75340_ratio.gff --perc 0.98 --t p --log 5 --num 3 --dist 250 --w 500 |
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426 |
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427 ----------------------------------------------------------------------------------------- |
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428 |
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429 eoc22334 |
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430 exit 0; |
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431 |
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432 } |