Mercurial > repos > matces > carpet_toolsuite
diff carpet-src-1/tools/CARPET/gff2bed_v2.xml @ 0:cdd489d98766
Migrated tool version 1.0.0 from old tool shed archive to new tool shed repository
author | matces |
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date | Tue, 07 Jun 2011 16:50:41 -0400 |
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--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/carpet-src-1/tools/CARPET/gff2bed_v2.xml Tue Jun 07 16:50:41 2011 -0400 @@ -0,0 +1,83 @@ +<tool id="gff to bed wiggle" name="Gff2Wig" version="1.1.0"> + <description>easy UCSC visualization of your raw-data</description> + <command interpreter="perl">gff2bed_v2.pl $input $col3 >$output</command> + <inputs> + <param format="gff" name="input" type="data" label="Source file"/> + <param name="col3" size="20" type="text" value="Analysis" label="Analysis name"/> + </inputs> + <outputs> + <data format="bed" name="output" file="wig-gff2bed.dat"/> + </outputs> + + <tests> + <test> + <param name="input" value="1.gff"/> + <output name="output" file="wig-gff2bed.dat"/> + </test> + </tests> + <help> +.. class:: infomark + +**What it does** + +This tool converts data from GFF format to WIGGLE format. This format allows the visuallization of raw intensity signals into the UCSC Genome Browser. + +PLEASE, for more detailed information refer to the CARPET user Manual: +click to download_ it. + +.. _download: /static/example_file/CARPET_userManual.zip + +-------- + +.. class:: infomark + +About formats + +**GFF** format General Feature Format is a format for describing genes and other features associated with DNA, RNA and Protein sequences. GFF lines have nine tab-separated fields: + +1. seqname - Must be a chromosome or scaffold. +2. source - The program that generated this feature. +3. feature - The name of this type of feature. Some examples of standard feature types are "CDS", "start_codon", "stop_codon", and "exon". +4. start - The starting position of the feature in the sequence. The first base is numbered 1. +5. end - The ending position of the feature (inclusive). +6. score - A score or signal. If there is no score value, enter ".". +7. strand - Valid entries include '+', '-', or '.' (for don't know/care). +8. frame - If the feature is a coding exon, frame should be a number between 0-2 that represents the reading frame of the first base. If the feature is not a coding exon, the value should be '.'. +9. group - All lines with the same group are linked together into a single item. + +-------- + + +**Example** + + +Nimblegen gives you back a GFF file with the coordinates of each probe and the normalized signal value --> log2(Cy5/Cy3) on the sixth column. + + +Click here_ to download a GFF file example. + +.. _here: /static/example_file/GFF_file_norm.txt.zip + +The following data in GFF format:: + + chr19 Nimblegen tiling_array 100000 1000051 -1.2 + . probe_name + chr19 Nimblegen tiling_array 100100 1000151 2.9 + . probe_name + +will be converted to WIG as shown below (Please note that a header will be added to the file):: + + track type=wiggle_0 name="Analysis name" description="raw_data ratio" visibility=full autoscale=off maxHeightPixels=100:50:20 color=200,100,0 altColor=0,100,200 + chr19 1000000 1000050 -1.2 + chr19 1000100 1000150 2.9 + +.. class:: infomark + +"Analysis name" will be shown in the UCSC Genome Browser as track name and can be defined by user. + +Viusalize chip raw intensity: + +.. image:: static/images/CARPET/ucsc2.jpg + + + + </help> +</tool>