0
|
1 <tool id="sra_pileup" name="Generate pileup format" version="1.1.1">
|
|
2 <description> from NCBI sra.</description>
|
|
3 <command>sra-pileup --log-level fatal
|
|
4 #if str( $region ) != "":
|
|
5 --aligned-region $region
|
|
6 #end if
|
|
7 #if str( $minMapq ) != "":
|
|
8 --minmapq $minMapq
|
|
9 #end if
|
|
10 #if $input.input_select == "file":
|
|
11 $input.file
|
|
12 #elif $input.input_select == "accession_number":
|
|
13 $input.accession
|
|
14 #elif $input.input_select == "text":
|
|
15 `cat $input.text`
|
|
16 #end if
|
|
17 > $output</command>
|
|
18 <version_string>sra-pileup --version</version_string>
|
|
19 <inputs>
|
|
20 <conditional name="input">
|
|
21 <param name="input_select" type="select" label="select input type">
|
|
22 <option value="accession_number">SRR accession</option>
|
|
23 <option value="file">SRA archive in current history</option>
|
|
24 <option value="text">text file containing SRR accession</option>
|
|
25 </param>
|
|
26 <when value="file">
|
|
27 <param format="sra" name="file" type="data" label="sra archive"/>
|
|
28 </when>
|
|
29 <when value="accession_number">
|
|
30 <param format="text" name="accession" type="text" label="accession"/>
|
|
31 </when>
|
|
32 <when value="text">
|
|
33 <param format="txt" name="text" type="data" label="text file"/>
|
|
34 </when>
|
|
35 </conditional>
|
|
36 <param format="text" name="region" type="text" label="aligned region"/>
|
|
37 <param format="text" name="minMapq" type="text" label="minimum mapping quality"/>
|
|
38 </inputs>
|
|
39 <outputs>
|
|
40 <data format="pileup" name="output"/>
|
|
41 </outputs>
|
|
42 <requirements>
|
|
43 <requirement type="package" version="2.3.3-3">sra_toolkit</requirement>
|
|
44 </requirements>
|
|
45 <help>
|
|
46 This tool produces pileup format from sra archives using sra-pileup.
|
|
47 Browse the NCBI SRA for SRR accessions at http://www.ncbi.nlm.nih.gov/Traces/sra/sra.cgi?view=studies.
|
|
48 The sra-pileup program is developed at NCBI, and is available at: http://www.ncbi.nlm.nih.gov/Traces/sra/sra.cgi?view=software.
|
|
49 Contact Matt Shirley at mdshw5@gmail.com for support and bug reports.
|
|
50 </help>
|
|
51 </tool>
|