Mercurial > repos > md-anderson-bioinformatics > heat_map_creation_advanced
view heatmap_advanced.sh @ 6:1f13d304ddbd draft
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author | insilico-bob |
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date | Thu, 20 Jun 2019 11:36:53 -0400 |
parents | 19382473a76b |
children | c5489978071a |
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#echo "1: " $1 " 2: " $2 " 3: " $3 " 4: " $4 " 5: " $5 " 6: " $6 " 7: " $7 " 8: " $8 " 9: " $9 " 10: " ${10} #echo "11: " ${11} " 12: " ${12} 13: " ${13} 14: " ${14} " 15: " ${15} " 16: " ${16} " 17: " ${17} " 18: " ${18} " 19: " ${19} " 20: " ${20} #echo "21: "${21}" 22: "${22}" 23: "${23}" 24: "${24}" 25: "${25}" 26: "${26}" 27: "${27}" 28: "${28}" 29: "${29}" 30: "${30} #Count total number of parameters, dataLayer parameters, and classification parameters parmSize=0 classSize=0 dataLayerSize=0 attribSize=0 for i in "$@"; do currParm=$(cut -d'|' -f1 <<< $i) parmSize=$((parmSize+1)) if [ $currParm = "classification" ] then classSize=$((classSize+1)) fi if [ $currParm = "matrix_files" ] then dataLayerSize=$((dataLayerSize+1)) fi if [ $currParm = "attribute" ] then attribSize=$((attribSize+1)) fi done if [ $dataLayerSize -lt 1 ] then noDataLayer="ERROR: No Heat Map Matrices provided. Please add at least one Heat Map Matrix to your request and try again." echo $noDataLayer exit $noDataLayer fi #Get tool data and tool install directories tooldir=$1 tooldata=$2 #create temp directory for row and col order and dendro files. tdir=$tooldata/$(date +%y%m%d%M%S) mkdir $tdir #echo "tdir: "$tdir #Extract parameters for row and column order and dendro files rowOrderFile=$tdir/ROfile.txt rowDendroFile=$tdir/RDfile.txt colOrderFile=$tdir/COfile.txt colDendroFile=$tdir/CDfile.txt rowOrderJson='"order_file": "'$rowOrderFile'",' rowDendroJson='"dendro_file": "'$rowDendroFile'",' colOrderJson='"order_file": "'$colOrderFile'",' colDendroJson='"dendro_file": "'$colDendroFile'",' #BEGIN: Construct JSON for all non-repeating parameters parmJson='{' rowConfigJson='"row_configuration": {' colConfigJson='"col_configuration": {' ctr=0 for i in "$@"; do if [ $ctr -gt 1 ] then currParm=$(cut -d'|' -f1 <<< $i) if [ $currParm != "matrix_files" ] && [ $currParm != "row_configuration" ] && [ $currParm != "col_configuration" ] && [ $currParm != "classification" ] && [ $currParm != "attribute" ] then #Parse pipe-delimited parameter parameter parmJson=$parmJson' "'$(cut -d'|' -f1 <<< $i)'":"'$(cut -d'|' -f2 <<< $i)'",' fi if [ $currParm = "row_configuration" ] then rowOrder=$(cut -d'|' -f3 <<< $i) rowDistance=$(cut -d'|' -f5 <<< $i) rowAgglomeration=$(cut -d'|' -f7 <<< $i) rowCuts=$(cut -d'|' -f9 <<< $i) rowLabels=$(cut -d'|' -f11 <<< $i) rowDataTypeJson='"'$(cut -d'|' -f10 <<< $i)'":["'$rowLabels'"],' rowCutType=$(cut -d'|' -f16 <<< $i) rowTopItemsJson='' rowTopItems=$(cut -d'|' -f13 <<< $i) if [ $rowTopItems != "None" ] && [ $rowTopItems != "" ] then rowTopItemsJson='"'$(cut -d'|' -f12 <<< $i)'": [' rowTopItems=${rowTopItems//,/'","'} rowTopItemsJson=$rowTopItemsJson'"'$rowTopItems'"],' fi rowCutsJson='' if [ $rowCutType != "none" ] then cutValues=$(cut -d'|' -f15 <<< $i) if [ $cutValues != "None" ] && [ $cutValues != "0" ] then if [ $rowCutType = "treecuts" ] then rowCutsJson=$rowCutsJson'"tree_cuts": "'$cutValues'",' rowCutsJson=$rowCutsJson'"cut_width": "5",' fi if [ $rowCutType = "positional" ] then rowCutErrorVal=0 [[ $cutValues != ?(-)+([0-9,]) ]] && rowCutErrorVal=$((rowCutErrorVal+1)) if [ $rowCutErrorVal -gt 0 ] then echo "GALAXY PARAMETER WARNING: Non-numeric values found for Row Fixed Gap Locations. Ignoring parameter value: "$cutValues else rowCutsJson=$rowCutsJson'"cut_locations": ['$cutValues'],' rowCutsJson=$rowCutsJson'"cut_width": "5",' fi fi fi fi rowConfigJson=$rowConfigJson$rowDataTypeJson$rowCutsJson$rowTopItemsJson if [ $rowOrder = 'Hierarchical' ] then rowConfigJson=$rowConfigJson$rowOrderJson$rowDendroJson fi rowConfigJson=$rowConfigJson' "'$(cut -d'|' -f2 <<< $i)'":"'$(cut -d'|' -f3 <<< $i)'","'$(cut -d'|' -f4 <<< $i)'":"'$(cut -d'|' -f5 <<< $i)'","'$(cut -d'|' -f6 <<< $i)'":"'$(cut -d'|' -f7 <<< $i)'","'$(cut -d'|' -f17 <<< $i)'":"'$(cut -d'|' -f18 <<< $i)'","'$(cut -d'|' -f19 <<< $i)'":"'$(cut -d'|' -f20 <<< $i)'"},' fi if [ $currParm = "col_configuration" ] then colOrder=$(cut -d'|' -f3 <<< $i) colDistance=$(cut -d'|' -f5 <<< $i) colAgglomeration=$(cut -d'|' -f7 <<< $i) colCuts=$(cut -d'|' -f9 <<< $i) colLabels=$(cut -d'|' -f11 <<< $i) colDataTypeJson='"'$(cut -d'|' -f10 <<< $i)'":["'$colLabels'"],' colCutType=$(cut -d'|' -f16 <<< $i) colTopItemsJson='' colTopItems=$(cut -d'|' -f13 <<< $i) if [ $colTopItems != "None" ] && [ $colTopItems != "" ] then colTopItemsJson='"'$(cut -d'|' -f12 <<< $i)'": [' colTopItems=${colTopItems//,/'","'} colTopItemsJson=$colTopItemsJson'"'$colTopItems'"],' fi colCutsJson='' if [ $colCutType != "none" ] then cutValues=$(cut -d'|' -f15 <<< $i) if [ $cutValues != "None" ] && [ $cutValues != "0" ] then if [ $colCutType = "treecuts" ] then colCutsJson=$colCutsJson'"tree_cuts": "'$cutValues'",' colCutsJson=$colCutsJson'"cut_width": "5",' fi if [ $colCutType = "positional" ] then colCutErrorVal=0 [[ $cutValues != ?(-)+([0-9,]) ]] && colCutErrorVal=$((colCutErrorVal+1)) if [ $colCutErrorVal -gt 0 ] then echo "GALAXY PARAMETER WARNING: Non-numeric values found for Column Fixed Gap Locations. Ignoring parameter value: "$cutValues else colCutsJson=$colCutsJson'"cut_locations": ['$cutValues'],' colCutsJson=$colCutsJson'"cut_width": "5",' fi fi fi fi colConfigJson=$colConfigJson$colDataTypeJson$colCutsJson$colTopItemsJson if [ $colOrder = 'Hierarchical' ] then colConfigJson=$colConfigJson$colOrderJson$colDendroJson fi colConfigJson=$colConfigJson' "'$(cut -d'|' -f2 <<< $i)'":"'$(cut -d'|' -f3 <<< $i)'","'$(cut -d'|' -f4 <<< $i)'":"'$(cut -d'|' -f5 <<< $i)'","'$(cut -d'|' -f6 <<< $i)'":"'$(cut -d'|' -f7 <<< $i)'","'$(cut -d'|' -f17 <<< $i)'":"'$(cut -d'|' -f18 <<< $i)'","'$(cut -d'|' -f19 <<< $i)'":"'$(cut -d'|' -f20 <<< $i)'"},' fi fi ctr=$((ctr+1)) done #END: Construct JSON for all non-repeating parameters #echo "rowOrder: "$rowOrder #echo "rowDistance: "$rowDistance #echo "rowAgglomeration: "$rowAgglomeration #echo "rowCuts: "$rowCuts #echo "rowLabels: "$rowLabels #echo "ROW CONFIG JSON: "$rowConfigJson #echo "colOrder: "$colOrder #echo "colDistance: "$colDistance #echo "colAgglomeration: "$colAgglomeration #echo "colCuts: "$colCuts #echo "colLabels: "$colLabels #echo "COL CONFIG JSON: "$colConfigJson #BEGIN: Construct JSON for data layers matrixJson='"matrix_files": [ ' inputMatrix='' dataLayerIter=0 dataLayerNames='' for i in "$@"; do currParm=$(cut -d'|' -f1 <<< $i) if [ $currParm = "matrix_files" ] then if [ $dataLayerIter -lt 1 ] then inputMatrix=$(cut -d'|' -f3 <<< $i) fi currMatrixName=$(cut -d'|' -f5 <<< $i) dataLayerIter=$((dataLayerIter+1)) if [[ $dataLayerNames =~ $currMatrixName ]] then currMatrixName=$currMatrixName$dataLayerIter fi dataLayerNames=$dataLayerNames$currMatrixName colorPref=$(cut -d'|' -f16 <<< $i) colorMapJson='' if [ $colorPref = "defined" ] then #validations to place leading zero on first breakpoint (if necessary) b1=$(cut -d'|' -f20 <<< $i) b1first=$(cut -d'.' -f1 <<< $b1) if [ $b1first = "-" ] then b1="-0."$(cut -d'.' -f2 <<< $b1) fi if [ "$b1first" = "" ] then b1="0"$b1 fi #validations to place leading zero on second breakpoint (if necessary) b2=$(cut -d'|' -f21 <<< $i) b2first=$(cut -d'.' -f1 <<< $b2) if [ $b2first = "-" ] then b2="-0."$(cut -d'.' -f2 <<< $b2) fi if [ "$b2first" = "" ] then b2="0"$b2 fi #validations to place leading zero on third breakpoint (if necessary) b3=$(cut -d'|' -f22 <<< $i) b3first=$(cut -d'.' -f1 <<< $b3) if [ $b3first = "-" ] then b3="-0."$(cut -d'.' -f2 <<< $b3) fi if [ "$b3first" = "" ] then b3="0"$b3 fi #validation to ensure that all entered breakpoints are numeric values regExp='^[+-]?([0-9]+\.?|[0-9]*\.[0-9]+)$' if [[ $b1 =~ $regExp ]] && [[ $b2 =~ $regExp ]] && [[ $b3 =~ $regExp ]] then colorMapJson=$colorMapJson'"color_map": {"colors": ["'$(cut -d'|' -f17 <<< $i)'","'$(cut -d'|' -f18 <<< $i)'","'$(cut -d'|' -f19 <<< $i)'"],' colorMapJson=$colorMapJson'"thresholds": ['$b1','$b2','$b3'],' colorMapJson=$colorMapJson'"missing":"'$(cut -d'|' -f23 <<< $i)'"},' else echo "GALAXY PARAMETER WARNING: Not all user-defined breakpoints are numbers. Defined breakpoints and colors will be ignored." fi fi #Parse pipe-delimited parameter parameter matrixJson=$matrixJson' {'$colorMapJson'"'$(cut -d'|' -f2 <<< $i)'":"'$(cut -d'|' -f3 <<< $i)'","'$(cut -d'|' -f4 <<< $i)'":"'$currMatrixName'","'$(cut -d'|' -f6 <<< $i)'":"'$(cut -d'|' -f7 <<< $i)'","'$(cut -d'|' -f8 <<< $i)'":"'$(cut -d'|' -f9 <<< $i)'","'$(cut -d'|' -f10 <<< $i)'":"'$(cut -d'|' -f11 <<< $i)'","'$(cut -d'|' -f12 <<< $i)'":"'$(cut -d'|' -f13 <<< $i)'","'$(cut -d'|' -f14 <<< $i)'":"'$(cut -d'|' -f15 <<< $i)'"}' if [ $dataLayerIter -lt $dataLayerSize ] then matrixJson=$matrixJson',' fi fi done matrixJson=$matrixJson"]," #END: Construct JSON for data layers #echo "DATA LAYER JSON: "$matrixJson #echo "INPUT MATRIX: "$inputMatrix #BEGIN: Construct JSON for attributes attribJson='"chm_attributes": [ ' attribIter=0 for i in "$@"; do currParm=$(cut -d'|' -f1 <<< $i) if [ $currParm = "attribute" ] then attribIter=$((attribIter+1)) attribParam=$(cut -d'|' -f2 <<< $i) #Parse pipe-delimited 2-part data layer parameter attribJson=$attribJson' {"'$(cut -d':' -f1 <<< $attribParam)'":"'$(cut -d':' -f2 <<< $attribParam)'"}' if [ $attribIter -lt $attribSize ] then attribJson=$attribJson',' fi fi done attribJson=$attribJson'],' #END: Construct JSON for attributes #echo "ATTRIB JSON: "$attribJson #BEGIN: Construct JSON for classification files classJson='"classification_files": [ ' colCutClass='' rowCutClass='' if [ $rowCuts -gt 1 ] then rowCutClass='{"name": "Class", "path": "'$tdir'/ROfile.txt.cut","position": "row", "color_map": {"type": "discrete"}, "bar_type": "color_plot"}' fi if [ $colCuts -gt 1 ] then if [ $rowCuts -gt 1 ] then rowCutClass=$rowCutClass',' fi colCutClass='{"name": "Class", "path": "'$tdir'/COfile.txt.cut","position": "column", "color_map": {"type": "discrete"}, "bar_type": "color_plot"}' if [ $classSize -gt 0 ] then colCutClass=$colCutClass',' fi else if [ $rowCuts -gt 1 ] && [ $classSize -gt 0 ] then rowCutClass=$rowCutClass',' fi fi classJson=$classJson$rowCutClass$colCutClass classIter=0 for i in "$@"; do currParm=$(cut -d'|' -f1 <<< $i) if [ $currParm = "classification" ] then classIter=$((classIter+1)) className=$(cut -d'|' -f3 <<< $i) #Parse pipe-delimited 3-part classification bar parameter classJson=$classJson' {"'$(cut -d'|' -f2 <<< $i)'":"'$(cut -d'|' -f3 <<< $i)'","'$(cut -d'|' -f4 <<< $i)'":"'$(cut -d'|' -f5 <<< $i)'","'$(cut -d'|' -f8 <<< $i)'":"'$(cut -d'|' -f9 <<< $i)'","'$(cut -d'|' -f12 <<< $i)'":"'$(cut -d'|' -f13 <<< $i)'","'$(cut -d'|' -f14 <<< $i)'":"'$(cut -d'|' -f15 <<< $i)'"' classCat=$(cut -d'|' -f7 <<< $i) classColorType=$(cut -d'_' -f2 <<< $classCat) classJson=$classJson',' classHeight=$(cut -d'|' -f11 <<< $i) heightErrorVal=0 [[ $classHeight != ?(-)+([0-9]) ]] && heightErrorVal=$((heightErrorVal+1)) if [ $heightErrorVal -gt 0 ] then echo 'GALAXY PARAMETER WARNING: Non-numeric values found for covariate bar ('$className') height. Height value ignored and default of 15 used: '$classHeight else classJson=$classJson'"height": "'$classHeight'",' fi classJson=$classJson' "position":"'$(cut -d'_' -f1 <<< $classCat)'","color_map": {"type":"'$classColorType'"}}' if [ $classIter -lt $classSize ] then classJson=$classJson',' fi fi done classJson=$classJson']' #END: Construct JSON for classification files #echo "CLASSIFICATION JSON: "$classJson #Complete construction of Parameter JSON file by adding all JSON sections created above parmJson=$parmJson$rowConfigJson$colConfigJson$attribJson$matrixJson$classJson parmJson=$parmJson'}' #echo "COMPLETED PARAMETER JSON: "$parmJson #run R to cluster matrix output="$(R --slave --vanilla --file=$tooldir/CHM_Advanced.R --args $inputMatrix $rowOrder $rowDistance $rowAgglomeration $colOrder $colDistance $colAgglomeration $rowOrderFile $colOrderFile $rowDendroFile $colDendroFile $rowCuts $colCuts $rowLabels $colLabels 2>&1)" # Check for errors from R step, log them if found, and exit script rc=$?; if [ $rc != 0 ] then echo $output; if [ `echo "$output" | grep -c "Inf in foreign function call"` -gt 0 ] then echo ""; echo "NOTE 1: This error can occur when a covariate file has inadvertently been selected as an Input Matrix. Check your Input Matrix entry."; echo "NOTE 2: This error can occur when there is no variation in a data rows or columns in the input matrix. Try a different distance measure or remove rows/columns without variation."; fi exit $rc; fi #Call java program to generate NGCHM viewer files. java -jar $tooldir/GalaxyMapGen.jar "$parmJson" #clean up tempdir rm -rf $tdir