Mercurial > repos > megan-shortridge > degenerateprimerremoval_fastq
diff cut_degen.xml @ 0:716cdcccc919 draft default tip
planemo upload for repository https://github.com/mshortr/degenerateprimerremoval_fastq commit 7d51a7e3ccb0164b530bf1664068d86241f2f2f5-dirty
author | megan-shortridge |
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date | Tue, 15 Sep 2015 14:14:41 -0400 |
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--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/cut_degen.xml Tue Sep 15 14:14:41 2015 -0400 @@ -0,0 +1,68 @@ +<tool id="cut_degen" name="Cut degenerate primers from dataset (for Fastq)" version="0.1.0"> + <description> Degenerate primer removal tool for Fastq files</description> + <requirements> + <requirement type = "package" version = "1.9">numpy</requirement> + <requirement type = "package" version = "1.65">biopython</requirement> + </requirements> + <stdio> + <exit_code range="1:" /> + </stdio> + + <command interpreter="python">cut_degen.py $input1 $input2 $input3 $input4 $input5 $input6 + </command> + <inputs> + <param name="input1" type="data" label="Input Fastq file" help="Please add a valid Fastq file!" format="fastq"/> + <param name="input2" type="text" label="Input adaptor" help="This will work also with degenerate adaptors. Type the adaptor here..." size="4x25" area="true"/> + <param name="input3" type="select" label="Keep reads without adaptor?" help="This is a True/False option, if True, reads that lack the adaptor will be kept. If false, they will be deleted from the dataset."> + <option value="True">True</option> + <option value="False">False</option> + </param> + <param name="input4" type="select" label = "Remove adaptors?" help="This is True/False option, if True, removes adaptors from sequences. If false, keeps the adaptors in the sequences."> + <option value="True">True</option> + <option value="False">False</option> + </param> + <param name="input5" type="select" multiple="False" label="5' or 3' end" help="Enter '5' to remove the primer from the 5' end, and enter '3' to remove the primer from the 3' end"> + <option value="5">5</option> + <option value="3">3</option> + </param> + <param name="input6" type="text" label="Adaptor name" help="Enter an identifier for your adaptor to be recorded in the output .txt file" size="4x25" area="true"> + </param> + </inputs> + <outputs> + <data name="output1" format="text" from_work_dir="output.txt" label="Degenerate adaptor removal on ${input1.name}(REPORT)"/> + <data name="output2" format="fastq" from_work_dir="output.fastq" label="Degenerate adaptor removal on ${input1.name}(READS)"/> + </outputs> + <tests> + <test> + <param name="input1" value="test.fastq"/> + <param name="input2" value="ACCTGCCTGCCG"/> + <param name="input3" value="True"/> + <param name="input4" value="True"/> + <param name="input5" value="5"/> + <param name="input6" value="UniA"/> + <output name="output1" file="output.txt"/> + <output name="output2" file="output.fastq"/> + </test> + <test> + <param name="input1" value="test3prime.fastq"/> + <param name="input2" value="GCTCGGTGGCGT"/> + <param name="input3" value="True"/> + <param name="input4" value="True"/> + <param name="input5" value="3"/> + <param name="input6" value="UniB"/> + <output name="output1" file="output2.txt"/> + <output name="output2" file="output2.fastq"/> + </test> + </tests> + <help> +This program takes Fastq files and removes a given degenerate primer sequence. There are a few arguments that you can put in: + 1) You can check whether or not to keep reads that do not have the adaptor in it. + 2) You can check whether or not to remove the adaptor sequences from your reads. + 3) You can choose whether to cut adaptors from the 5', or 3' end of the molecules. + 4) You can add an identifier for the adaptor which will show up in the output text file. + + </help> + <citations> + <citation type="doi">10.1093/bioinformatics/btp163</citation> + </citations> +</tool> \ No newline at end of file