Mercurial > repos > melpetera > batchcorrection
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author | melpetera |
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date | Thu, 14 Jan 2021 09:56:58 +0000 |
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#!/usr/bin/env Rscript ################################################################################################ # batch_correction_wrapper # # # # Authors: Marion LANDI / Jean-Francois MARTIN / Melanie Petera # # User: Galaxy # # Original data: -- # # Starting date: 22-07-2014 # # Version 1: 22-07-2014 # # Version 2: 08-12-2014 # # Version 2.1: 09-01-2015 modification in Error message of sample matching # # Version 2.2: 16-03-2015 inclusion of miniTools' functions for special characters # # Version 2.90: 18-08-2015 new parameter valnull # # Version 2.91: 25-08-2016 error message improvment # # Version 3: 02-10-2020 # # - split of tool-linked code and script-linked one # # - addition of args print and sessionInfo() # # - adjustment of sample tags' parameters to 3L methods # # - addition of the min.norm argument in meth3L() call # # # # Input files: dataMatrix.txt, sampleMetadata.txt, variableMetadata.txt (BC only) # # Output files: graph.pdf, corrected table (BC only), diagnostic table (DBC only), # # variableMetadata (BC only) # # # ################################################################################################ library(batch) #necessary for parseCommandArgs function ##------------------------------ ## test help option ##------------------------------ # Prog. constants argv.help <- commandArgs(trailingOnly = FALSE) script.path <- sub("--file=", "", argv.help[grep("--file=", argv.help)]) prog.name <- basename(script.path) # Test Help if (length(grep('-h', argv.help)) > 0) { cat("Usage: Rscript ", prog.name, "{args} \n", "parameters: \n", "\tanalyse {val}: must be set to \"batch_correction\"", "\tdataMatrix {file}: set the input data matrix file (mandatory) \n", "\tsampleMetadata {file}: set the input sample metadata file (mandatory) \n", "\tvariableMetadata {file}: set the input variable metadata file (mandatory) \n", "\tmethod {opt}: set the method; can set to \"linear\", \"lowess\" or \"loess\" (mandatory) \n", "\tspan {condition}: set the span condition; set to \"none\" if method is set to \"linear\" (mandatory) \n", "\tref_factor {value}: set the ref_factor value; (if span value is set to NULL, optional) \n", "\tdetail {value}: set the detail value; (if span value is set to NULL, optional) \n", "\tdataMatrix_out {file}: set the output data matrix file (mandatory) \n", "\tvariableMetadata_out {file}: set the output variable metadata file (mandatory) \n", "\tgraph_output {file}: set the output graph file (mandatory) \n", "\trdata_output {file}: set the output Rdata file (mandatory) \n", "\tbatch_col_name {val}: the column name for batch. Default value is \"batch\".\n", "\tinjection_order_col_name {val}: the column name for the injection order. Default value is \"injectionOrder\".\n", "\tsample_type_col_name {val}: the column name for the sample types. Default value is \"sampleType\".\n", "\tsample_type_tags {val}: the tags used inside the sample type column, defined as key/value pairs separated by commas (example: blank=blank,pool=pool,sample=sample).\n", "\n") quit(status = 0) } ##------------------------------ ## init. params ##------------------------------ args = parseCommandArgs(evaluate=FALSE) #interpretation of arguments given in command line as an R list of objects cat('\nJob starting time:\n',format(Sys.time(), "%a %d %b %Y %X"), '\n\n--------------------------------------------------------------------', '\nParameters used:\n\n') print(args) cat('--------------------------------------------------------------------\n\n') # Set default col names if ( ! 'batch_col_name' %in% names(args)) args[['batch_col_name']] <- 'batch' if ( ! 'injection_order_col_name' %in% names(args)) args[['injection_order_col_name']] <- 'injectionOrder' if ( ! 'sample_type_col_name' %in% names(args)) args[['sample_type_col_name']] <- 'sampleType' if ( ! 'sample_type_tags' %in% names(args)) args[['sample_type_tags']] <- 'blank=blank,pool=pool,sample=sample' # Parse sample type tags sample.type.tags <- list() for (kv in strsplit(strsplit(args$sample_type_tags, ',')[[1]], '=')) sample.type.tags[[kv[[1]]]] <- kv[-1] if ( ! all(c('pool', 'blank', 'sample') %in% names(sample.type.tags))) stop("All tags pool, blank and sample must be defined in option sampleTypeTags.") args$sample_type_tags <- sample.type.tags ##------------------------------ ## init. functions ##------------------------------ source_local <- function(...){ argv <- commandArgs(trailingOnly = FALSE) base_dir <- dirname(substring(argv[grep("--file=", argv)], 8)) for(i in 1:length(list(...))){source(paste(base_dir, list(...)[[i]], sep="/"))} } #Import the different functions source_local("batch_correction_3Lfct.R","batch_correction_3Llauncher.R","easyrlibrary-lib/RcheckLibrary.R","easyrlibrary-lib/miniTools.R") # Specificities of BC and DBC if(args$analyse == "batch_correction") { args$out_graph_pdf <- NULL args$out_preNormSummary <- NULL }else{ args$variableMetadata <- NULL args$rdata_output <- NULL args$dataMatrix_out <- NULL args$variableMetadata_out <- NULL args$graph_output <- NULL args$method <- NULL args$detail <- NULL args$valnull <- NULL } # Launch tool meth3L(idsample=args$sampleMetadata, iddata=args$dataMatrix, sample_type_col_name=args$sample_type_col_name, injection_order_col_name=args$injection_order_col_name, batch_col_name=args$batch_col_name, sample_type_tags=args$sample_type_tags, factbio=args$ref_factor, analyse=args$analyse, metaion=args$variableMetadata, detail=args$detail, method=args$method, outlog=args$graph_output, span=args$span, valnull=args$valnull, rdata_output=args$rdata_output, dataMatrix_out=args$dataMatrix_out, variableMetadata_out=args$variableMetadata_out, out_graph_pdf=args$out_graph_pdf, out_preNormSummary=args$out_preNormSummary, min.norm=1) cat('\n\n--------------------------------------------------------------------', '\nInformation about R (version, Operating System, attached or loaded packages):\n\n') sessionInfo() cat('--------------------------------------------------------------------\n', '\nJob ending time:\n',format(Sys.time(), "%a %d %b %Y %X")) rm(args)