Mercurial > repos > mheinzl > variant_analyzer2

annotate mut2sscs.py @ 86:97bd9c7a1b44 draft

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planemo upload for repository https://github.com/Single-Molecule-Genetics/VariantAnalyzerGalaxy/tree/master/tools/variant_analyzer commit ee4a8e6cf290e6c8a4d55f9cd2839d60ab3b11c8-dirty
author mheinzl
date Sat, 22 Apr 2023 09:28:36 +0000
parents fdfe9a919ff7
children
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1 #!/usr/bin/env python
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2
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3 """mut2sscs.py
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5 Author -- Gundula Povysil
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6 Contact -- povysil@bioinf.jku.at
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8 Takes a tabular file with mutations from DCS and a BAM file of SSCS as input
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9 and extracts all tags of reads that carry the mutation.
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10 Calculates statistics about number of ab/ba/duplex per mutation.
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12 ======= ========== ================= ================================
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13 Version Date Author Description
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14 0.2.1 2019-10-27 Gundula Povysil -
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15 ======= ========== ================= ================================
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17 USAGE: python mut2sscs.py DCS_Mutations.tabular SSCS.bam SSCS_counts.json
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19 """
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21 from __future__ import division
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23 import argparse
e5953c54cfb5 planemo upload for repository https://github.com/gpovysil/VariantAnalyzerGalaxy/tree/master/tools/variant_analyzer commit ee4a8e6cf290e6c8a4d55f9cd2839d60ab3b11c8
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24 import json
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25 import os
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26 import re
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27 import sys
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28
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29 import numpy as np
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30 import pysam
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31 from cyvcf2 import VCF
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34 def make_argparser():
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35 parser = argparse.ArgumentParser(description='Takes a vcf file with mutations and a BAM file as input and prints all tags of reads that carry the mutation to a user specified output file.')
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36 parser.add_argument('--mutFile',
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37 help='VCR file with DCS mutations.')
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38 parser.add_argument('--bamFile',
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39 help='BAM file with aligned SSCS reads.')
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40 parser.add_argument('--outputJson',
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41 help='Output JSON file to store SSCS counts.')
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42 return parser
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45 def mut2sscs(argv):
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46 parser = make_argparser()
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47 args = parser.parse_args(argv[1:])
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49 file1 = args.mutFile
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50 file2 = args.bamFile
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51 sscs_counts_json = args.outputJson
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53 if os.path.isfile(file1) is False:
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54 sys.exit("Error: Could not find '{}'".format(file1))
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56 if os.path.isfile(file2) is False:
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57 sys.exit("Error: Could not find '{}'".format(file2))
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58
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59 # read SSCS bam file
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60 # pysam.index(file2)
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61 bam = pysam.AlignmentFile(file2, "rb")
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63 # get tags
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64 mut_pos_dict = {}
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65 ref_pos_dict = {}
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66
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67 for variant in VCF(file1):
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68 chrom = variant.CHROM
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69 stop_pos = variant.start
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70 ref = variant.REF
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71 if len(variant.ALT) == 0:
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72 continue
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73 else:
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74 alt = variant.ALT[0]
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75 alt = alt.upper()
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76 ref = ref.upper()
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77 if "N" in alt: # skip indels with N in alt allele --> it is not an indel but just a mismatch at the position where the N is (checked this in IGV)
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78 continue
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79 chrom_stop_pos = str(chrom) + "#" + str(stop_pos) + "#" + ref + "#" + alt
78
fdfe9a919ff7 planemo upload for repository https://github.com/Single-Molecule-Genetics/VariantAnalyzerGalaxy/tree/master/tools/variant_analyzer commit ee4a8e6cf290e6c8a4d55f9cd2839d60ab3b11c8-dirty
mheinzl
parents: 75
diff changeset
80 for pileupcolumn in bam.pileup(chrom, stop_pos - 1, stop_pos + 1, max_depth=1000000000):
fdfe9a919ff7 planemo upload for repository https://github.com/Single-Molecule-Genetics/VariantAnalyzerGalaxy/tree/master/tools/variant_analyzer commit ee4a8e6cf290e6c8a4d55f9cd2839d60ab3b11c8-dirty
mheinzl
parents: 75
diff changeset
81 if pileupcolumn.reference_pos == stop_pos:
fdfe9a919ff7 planemo upload for repository https://github.com/Single-Molecule-Genetics/VariantAnalyzerGalaxy/tree/master/tools/variant_analyzer commit ee4a8e6cf290e6c8a4d55f9cd2839d60ab3b11c8-dirty
mheinzl
parents: 75
diff changeset
82 count_alt = 0
fdfe9a919ff7 planemo upload for repository https://github.com/Single-Molecule-Genetics/VariantAnalyzerGalaxy/tree/master/tools/variant_analyzer commit ee4a8e6cf290e6c8a4d55f9cd2839d60ab3b11c8-dirty
mheinzl
parents: 75
diff changeset
83 count_ref = 0
fdfe9a919ff7 planemo upload for repository https://github.com/Single-Molecule-Genetics/VariantAnalyzerGalaxy/tree/master/tools/variant_analyzer commit ee4a8e6cf290e6c8a4d55f9cd2839d60ab3b11c8-dirty
mheinzl
parents: 75
diff changeset
84 count_indel = 0
fdfe9a919ff7 planemo upload for repository https://github.com/Single-Molecule-Genetics/VariantAnalyzerGalaxy/tree/master/tools/variant_analyzer commit ee4a8e6cf290e6c8a4d55f9cd2839d60ab3b11c8-dirty
mheinzl
parents: 75
diff changeset
85 for pileupread in pileupcolumn.pileups:
fdfe9a919ff7 planemo upload for repository https://github.com/Single-Molecule-Genetics/VariantAnalyzerGalaxy/tree/master/tools/variant_analyzer commit ee4a8e6cf290e6c8a4d55f9cd2839d60ab3b11c8-dirty
mheinzl
parents: 75
diff changeset
86 if not pileupread.is_refskip:
fdfe9a919ff7 planemo upload for repository https://github.com/Single-Molecule-Genetics/VariantAnalyzerGalaxy/tree/master/tools/variant_analyzer commit ee4a8e6cf290e6c8a4d55f9cd2839d60ab3b11c8-dirty
mheinzl
parents: 75
diff changeset
87 tag = pileupread.alignment.query_name
fdfe9a919ff7 planemo upload for repository https://github.com/Single-Molecule-Genetics/VariantAnalyzerGalaxy/tree/master/tools/variant_analyzer commit ee4a8e6cf290e6c8a4d55f9cd2839d60ab3b11c8-dirty
mheinzl
parents: 75
diff changeset
88 abba = tag[-2:]
fdfe9a919ff7 planemo upload for repository https://github.com/Single-Molecule-Genetics/VariantAnalyzerGalaxy/tree/master/tools/variant_analyzer commit ee4a8e6cf290e6c8a4d55f9cd2839d60ab3b11c8-dirty
mheinzl
parents: 75
diff changeset
89 if pileupread.is_del:
fdfe9a919ff7 planemo upload for repository https://github.com/Single-Molecule-Genetics/VariantAnalyzerGalaxy/tree/master/tools/variant_analyzer commit ee4a8e6cf290e6c8a4d55f9cd2839d60ab3b11c8-dirty
mheinzl
parents: 75
diff changeset
90 p = pileupread.query_position_or_next
fdfe9a919ff7 planemo upload for repository https://github.com/Single-Molecule-Genetics/VariantAnalyzerGalaxy/tree/master/tools/variant_analyzer commit ee4a8e6cf290e6c8a4d55f9cd2839d60ab3b11c8-dirty
mheinzl
parents: 75
diff changeset
91 e = p + len(alt) - 1
fdfe9a919ff7 planemo upload for repository https://github.com/Single-Molecule-Genetics/VariantAnalyzerGalaxy/tree/master/tools/variant_analyzer commit ee4a8e6cf290e6c8a4d55f9cd2839d60ab3b11c8-dirty
mheinzl
parents: 75
diff changeset
92 else:
fdfe9a919ff7 planemo upload for repository https://github.com/Single-Molecule-Genetics/VariantAnalyzerGalaxy/tree/master/tools/variant_analyzer commit ee4a8e6cf290e6c8a4d55f9cd2839d60ab3b11c8-dirty
mheinzl
parents: 75
diff changeset
93 p = pileupread.query_position
fdfe9a919ff7 planemo upload for repository https://github.com/Single-Molecule-Genetics/VariantAnalyzerGalaxy/tree/master/tools/variant_analyzer commit ee4a8e6cf290e6c8a4d55f9cd2839d60ab3b11c8-dirty
mheinzl
parents: 75
diff changeset
94 e = p + len(alt)
fdfe9a919ff7 planemo upload for repository https://github.com/Single-Molecule-Genetics/VariantAnalyzerGalaxy/tree/master/tools/variant_analyzer commit ee4a8e6cf290e6c8a4d55f9cd2839d60ab3b11c8-dirty
mheinzl
parents: 75
diff changeset
95 tag = pileupread.alignment.query_name
fdfe9a919ff7 planemo upload for repository https://github.com/Single-Molecule-Genetics/VariantAnalyzerGalaxy/tree/master/tools/variant_analyzer commit ee4a8e6cf290e6c8a4d55f9cd2839d60ab3b11c8-dirty
mheinzl
parents: 75
diff changeset
96 if "_" in tag:
fdfe9a919ff7 planemo upload for repository https://github.com/Single-Molecule-Genetics/VariantAnalyzerGalaxy/tree/master/tools/variant_analyzer commit ee4a8e6cf290e6c8a4d55f9cd2839d60ab3b11c8-dirty
mheinzl
parents: 75
diff changeset
97 tag = re.split('_', tag)[0]
fdfe9a919ff7 planemo upload for repository https://github.com/Single-Molecule-Genetics/VariantAnalyzerGalaxy/tree/master/tools/variant_analyzer commit ee4a8e6cf290e6c8a4d55f9cd2839d60ab3b11c8-dirty
mheinzl
parents: 75
diff changeset
98 s = p
fdfe9a919ff7 planemo upload for repository https://github.com/Single-Molecule-Genetics/VariantAnalyzerGalaxy/tree/master/tools/variant_analyzer commit ee4a8e6cf290e6c8a4d55f9cd2839d60ab3b11c8-dirty
mheinzl
parents: 75
diff changeset
99 split_cigar = re.split('(\d+)', pileupread.alignment.cigarstring)
fdfe9a919ff7 planemo upload for repository https://github.com/Single-Molecule-Genetics/VariantAnalyzerGalaxy/tree/master/tools/variant_analyzer commit ee4a8e6cf290e6c8a4d55f9cd2839d60ab3b11c8-dirty
mheinzl
parents: 75
diff changeset
100 if len(ref) < len(alt): # insertion
fdfe9a919ff7 planemo upload for repository https://github.com/Single-Molecule-Genetics/VariantAnalyzerGalaxy/tree/master/tools/variant_analyzer commit ee4a8e6cf290e6c8a4d55f9cd2839d60ab3b11c8-dirty
mheinzl
parents: 75
diff changeset
101 if "I" in split_cigar:
fdfe9a919ff7 planemo upload for repository https://github.com/Single-Molecule-Genetics/VariantAnalyzerGalaxy/tree/master/tools/variant_analyzer commit ee4a8e6cf290e6c8a4d55f9cd2839d60ab3b11c8-dirty
mheinzl
parents: 75
diff changeset
102 all_insertions = [inser_i for inser_i, ins in enumerate(split_cigar) if ins == "I"]
fdfe9a919ff7 planemo upload for repository https://github.com/Single-Molecule-Genetics/VariantAnalyzerGalaxy/tree/master/tools/variant_analyzer commit ee4a8e6cf290e6c8a4d55f9cd2839d60ab3b11c8-dirty
mheinzl
parents: 75
diff changeset
103 for ai in all_insertions: # if multiple insertions in DCS
fdfe9a919ff7 planemo upload for repository https://github.com/Single-Molecule-Genetics/VariantAnalyzerGalaxy/tree/master/tools/variant_analyzer commit ee4a8e6cf290e6c8a4d55f9cd2839d60ab3b11c8-dirty
mheinzl
parents: 75
diff changeset
104 ins_index = [int(ci) for ci in split_cigar[:ai - 1] if ci.isdigit()]
fdfe9a919ff7 planemo upload for repository https://github.com/Single-Molecule-Genetics/VariantAnalyzerGalaxy/tree/master/tools/variant_analyzer commit ee4a8e6cf290e6c8a4d55f9cd2839d60ab3b11c8-dirty
mheinzl
parents: 75
diff changeset
105 ins_count = split_cigar[ai - 1] # nr of insertions should match with alt allele
fdfe9a919ff7 planemo upload for repository https://github.com/Single-Molecule-Genetics/VariantAnalyzerGalaxy/tree/master/tools/variant_analyzer commit ee4a8e6cf290e6c8a4d55f9cd2839d60ab3b11c8-dirty
mheinzl
parents: 75
diff changeset
106 if "I" in split_cigar and sum(ins_index) == p + 1 and len(alt) - 1 == int(ins_count):
fdfe9a919ff7 planemo upload for repository https://github.com/Single-Molecule-Genetics/VariantAnalyzerGalaxy/tree/master/tools/variant_analyzer commit ee4a8e6cf290e6c8a4d55f9cd2839d60ab3b11c8-dirty
mheinzl
parents: 75
diff changeset
107 nuc = pileupread.alignment.query_sequence[s:e]
fdfe9a919ff7 planemo upload for repository https://github.com/Single-Molecule-Genetics/VariantAnalyzerGalaxy/tree/master/tools/variant_analyzer commit ee4a8e6cf290e6c8a4d55f9cd2839d60ab3b11c8-dirty
mheinzl
parents: 75
diff changeset
108 break
6
11a2a34f8a2b planemo upload for repository https://github.com/gpovysil/VariantAnalyzerGalaxy/tree/master/tools/variant_analyzer commit ee4a8e6cf290e6c8a4d55f9cd2839d60ab3b11c8
mheinzl
parents: 0
diff changeset
109 else:
78
fdfe9a919ff7 planemo upload for repository https://github.com/Single-Molecule-Genetics/VariantAnalyzerGalaxy/tree/master/tools/variant_analyzer commit ee4a8e6cf290e6c8a4d55f9cd2839d60ab3b11c8-dirty
mheinzl
parents: 75
diff changeset
110 nuc = pileupread.alignment.query_sequence[s]
fdfe9a919ff7 planemo upload for repository https://github.com/Single-Molecule-Genetics/VariantAnalyzerGalaxy/tree/master/tools/variant_analyzer commit ee4a8e6cf290e6c8a4d55f9cd2839d60ab3b11c8-dirty
mheinzl
parents: 75
diff changeset
111 else:
fdfe9a919ff7 planemo upload for repository https://github.com/Single-Molecule-Genetics/VariantAnalyzerGalaxy/tree/master/tools/variant_analyzer commit ee4a8e6cf290e6c8a4d55f9cd2839d60ab3b11c8-dirty
mheinzl
parents: 75
diff changeset
112 nuc = pileupread.alignment.query_sequence[s]
fdfe9a919ff7 planemo upload for repository https://github.com/Single-Molecule-Genetics/VariantAnalyzerGalaxy/tree/master/tools/variant_analyzer commit ee4a8e6cf290e6c8a4d55f9cd2839d60ab3b11c8-dirty
mheinzl
parents: 75
diff changeset
113 elif len(ref) > len(alt): # deletion
fdfe9a919ff7 planemo upload for repository https://github.com/Single-Molecule-Genetics/VariantAnalyzerGalaxy/tree/master/tools/variant_analyzer commit ee4a8e6cf290e6c8a4d55f9cd2839d60ab3b11c8-dirty
mheinzl
parents: 75
diff changeset
114 ref_positions = pileupread.alignment.get_reference_positions(full_length=True)[s:p + len(ref)]
fdfe9a919ff7 planemo upload for repository https://github.com/Single-Molecule-Genetics/VariantAnalyzerGalaxy/tree/master/tools/variant_analyzer commit ee4a8e6cf290e6c8a4d55f9cd2839d60ab3b11c8-dirty
mheinzl
parents: 75
diff changeset
115 if "D" in split_cigar:
fdfe9a919ff7 planemo upload for repository https://github.com/Single-Molecule-Genetics/VariantAnalyzerGalaxy/tree/master/tools/variant_analyzer commit ee4a8e6cf290e6c8a4d55f9cd2839d60ab3b11c8-dirty
mheinzl
parents: 75
diff changeset
116 all_deletions = [del_i for del_i, dele in enumerate(split_cigar) if dele == "D"]
fdfe9a919ff7 planemo upload for repository https://github.com/Single-Molecule-Genetics/VariantAnalyzerGalaxy/tree/master/tools/variant_analyzer commit ee4a8e6cf290e6c8a4d55f9cd2839d60ab3b11c8-dirty
mheinzl
parents: 75
diff changeset
117 for di, ai in enumerate(all_deletions): # if multiple insertions in DCS
fdfe9a919ff7 planemo upload for repository https://github.com/Single-Molecule-Genetics/VariantAnalyzerGalaxy/tree/master/tools/variant_analyzer commit ee4a8e6cf290e6c8a4d55f9cd2839d60ab3b11c8-dirty
mheinzl
parents: 75
diff changeset
118 if di > 0: # more than 1 deletion, don't count previous deletion to position
fdfe9a919ff7 planemo upload for repository https://github.com/Single-Molecule-Genetics/VariantAnalyzerGalaxy/tree/master/tools/variant_analyzer commit ee4a8e6cf290e6c8a4d55f9cd2839d60ab3b11c8-dirty
mheinzl
parents: 75
diff changeset
119 all_deletions_mod = split_cigar[:ai - 1]
fdfe9a919ff7 planemo upload for repository https://github.com/Single-Molecule-Genetics/VariantAnalyzerGalaxy/tree/master/tools/variant_analyzer commit ee4a8e6cf290e6c8a4d55f9cd2839d60ab3b11c8-dirty
mheinzl
parents: 75
diff changeset
120 prev_del_idx = [all_deletions_mod.index("D") - 1, all_deletions_mod.index("D")]
fdfe9a919ff7 planemo upload for repository https://github.com/Single-Molecule-Genetics/VariantAnalyzerGalaxy/tree/master/tools/variant_analyzer commit ee4a8e6cf290e6c8a4d55f9cd2839d60ab3b11c8-dirty
mheinzl
parents: 75
diff changeset
121 split_cigar_no_prev = [ad for i, ad in enumerate(all_deletions_mod) if i not in prev_del_idx]
fdfe9a919ff7 planemo upload for repository https://github.com/Single-Molecule-Genetics/VariantAnalyzerGalaxy/tree/master/tools/variant_analyzer commit ee4a8e6cf290e6c8a4d55f9cd2839d60ab3b11c8-dirty
mheinzl
parents: 75
diff changeset
122 del_index = [int(ci) for ci in split_cigar_no_prev[:ai - 1] if ci.isdigit()]
fdfe9a919ff7 planemo upload for repository https://github.com/Single-Molecule-Genetics/VariantAnalyzerGalaxy/tree/master/tools/variant_analyzer commit ee4a8e6cf290e6c8a4d55f9cd2839d60ab3b11c8-dirty
mheinzl
parents: 75
diff changeset
123 else: # first deletion in read, sum all previous (mis)matches and insertions to position
fdfe9a919ff7 planemo upload for repository https://github.com/Single-Molecule-Genetics/VariantAnalyzerGalaxy/tree/master/tools/variant_analyzer commit ee4a8e6cf290e6c8a4d55f9cd2839d60ab3b11c8-dirty
mheinzl
parents: 75
diff changeset
124 del_index = [int(ci) for ci in split_cigar[:ai - 1] if ci.isdigit()]
fdfe9a919ff7 planemo upload for repository https://github.com/Single-Molecule-Genetics/VariantAnalyzerGalaxy/tree/master/tools/variant_analyzer commit ee4a8e6cf290e6c8a4d55f9cd2839d60ab3b11c8-dirty
mheinzl
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diff changeset
125 del_count = split_cigar[ai - 1] # nr of deletions should match with ref allele
fdfe9a919ff7 planemo upload for repository https://github.com/Single-Molecule-Genetics/VariantAnalyzerGalaxy/tree/master/tools/variant_analyzer commit ee4a8e6cf290e6c8a4d55f9cd2839d60ab3b11c8-dirty
mheinzl
parents: 75
diff changeset
126 if "D" in split_cigar and sum(del_index) == p + 1 and len(ref) - 1 == int(del_count):
fdfe9a919ff7 planemo upload for repository https://github.com/Single-Molecule-Genetics/VariantAnalyzerGalaxy/tree/master/tools/variant_analyzer commit ee4a8e6cf290e6c8a4d55f9cd2839d60ab3b11c8-dirty
mheinzl
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diff changeset
127 nuc = pileupread.alignment.query_sequence[s:e]
fdfe9a919ff7 planemo upload for repository https://github.com/Single-Molecule-Genetics/VariantAnalyzerGalaxy/tree/master/tools/variant_analyzer commit ee4a8e6cf290e6c8a4d55f9cd2839d60ab3b11c8-dirty
mheinzl
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diff changeset
128 if nuc == "":
fdfe9a919ff7 planemo upload for repository https://github.com/Single-Molecule-Genetics/VariantAnalyzerGalaxy/tree/master/tools/variant_analyzer commit ee4a8e6cf290e6c8a4d55f9cd2839d60ab3b11c8-dirty
mheinzl
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diff changeset
129 nuc = str(alt)
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mheinzl
parents: 75
diff changeset
130 break
fdfe9a919ff7 planemo upload for repository https://github.com/Single-Molecule-Genetics/VariantAnalyzerGalaxy/tree/master/tools/variant_analyzer commit ee4a8e6cf290e6c8a4d55f9cd2839d60ab3b11c8-dirty
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diff changeset
131 else:
fdfe9a919ff7 planemo upload for repository https://github.com/Single-Molecule-Genetics/VariantAnalyzerGalaxy/tree/master/tools/variant_analyzer commit ee4a8e6cf290e6c8a4d55f9cd2839d60ab3b11c8-dirty
mheinzl
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diff changeset
132 nuc = pileupread.alignment.query_sequence[s:s + len(ref)]
fdfe9a919ff7 planemo upload for repository https://github.com/Single-Molecule-Genetics/VariantAnalyzerGalaxy/tree/master/tools/variant_analyzer commit ee4a8e6cf290e6c8a4d55f9cd2839d60ab3b11c8-dirty
mheinzl
parents: 75
diff changeset
133 elif len(ref_positions) < len(ref): # DCS has reference but the position is at the very end of the DCS and therefore not the full reference positions are there
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mheinzl
parents: 75
diff changeset
134 nuc = pileupread.alignment.get_reference_sequence()[s:s + len(ref)]
fdfe9a919ff7 planemo upload for repository https://github.com/Single-Molecule-Genetics/VariantAnalyzerGalaxy/tree/master/tools/variant_analyzer commit ee4a8e6cf290e6c8a4d55f9cd2839d60ab3b11c8-dirty
mheinzl
parents: 75
diff changeset
135 if nuc.upper() == ref[:len(nuc)]:
fdfe9a919ff7 planemo upload for repository https://github.com/Single-Molecule-Genetics/VariantAnalyzerGalaxy/tree/master/tools/variant_analyzer commit ee4a8e6cf290e6c8a4d55f9cd2839d60ab3b11c8-dirty
mheinzl
parents: 75
diff changeset
136 nuc = str(ref)
fdfe9a919ff7 planemo upload for repository https://github.com/Single-Molecule-Genetics/VariantAnalyzerGalaxy/tree/master/tools/variant_analyzer commit ee4a8e6cf290e6c8a4d55f9cd2839d60ab3b11c8-dirty
mheinzl
parents: 75
diff changeset
137 else:
fdfe9a919ff7 planemo upload for repository https://github.com/Single-Molecule-Genetics/VariantAnalyzerGalaxy/tree/master/tools/variant_analyzer commit ee4a8e6cf290e6c8a4d55f9cd2839d60ab3b11c8-dirty
mheinzl
parents: 75
diff changeset
138 nuc = pileupread.alignment.query_sequence[s:s + len(ref)]
fdfe9a919ff7 planemo upload for repository https://github.com/Single-Molecule-Genetics/VariantAnalyzerGalaxy/tree/master/tools/variant_analyzer commit ee4a8e6cf290e6c8a4d55f9cd2839d60ab3b11c8-dirty
mheinzl
parents: 75
diff changeset
139 else: # SNV: query position is None if is_del or is_refskip is set.
fdfe9a919ff7 planemo upload for repository https://github.com/Single-Molecule-Genetics/VariantAnalyzerGalaxy/tree/master/tools/variant_analyzer commit ee4a8e6cf290e6c8a4d55f9cd2839d60ab3b11c8-dirty
mheinzl
parents: 75
diff changeset
140 nuc = pileupread.alignment.query_sequence[s]
6
11a2a34f8a2b planemo upload for repository https://github.com/gpovysil/VariantAnalyzerGalaxy/tree/master/tools/variant_analyzer commit ee4a8e6cf290e6c8a4d55f9cd2839d60ab3b11c8
mheinzl
parents: 0
diff changeset
141
78
fdfe9a919ff7 planemo upload for repository https://github.com/Single-Molecule-Genetics/VariantAnalyzerGalaxy/tree/master/tools/variant_analyzer commit ee4a8e6cf290e6c8a4d55f9cd2839d60ab3b11c8-dirty
mheinzl
parents: 75
diff changeset
142 nuc = nuc.upper()
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mheinzl
parents: 75
diff changeset
143
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mheinzl
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diff changeset
144 if nuc == alt:
fdfe9a919ff7 planemo upload for repository https://github.com/Single-Molecule-Genetics/VariantAnalyzerGalaxy/tree/master/tools/variant_analyzer commit ee4a8e6cf290e6c8a4d55f9cd2839d60ab3b11c8-dirty
mheinzl
parents: 75
diff changeset
145 count_alt += 1
fdfe9a919ff7 planemo upload for repository https://github.com/Single-Molecule-Genetics/VariantAnalyzerGalaxy/tree/master/tools/variant_analyzer commit ee4a8e6cf290e6c8a4d55f9cd2839d60ab3b11c8-dirty
mheinzl
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diff changeset
146 if chrom_stop_pos in mut_pos_dict:
fdfe9a919ff7 planemo upload for repository https://github.com/Single-Molecule-Genetics/VariantAnalyzerGalaxy/tree/master/tools/variant_analyzer commit ee4a8e6cf290e6c8a4d55f9cd2839d60ab3b11c8-dirty
mheinzl
parents: 75
diff changeset
147 if abba in mut_pos_dict[chrom_stop_pos]:
fdfe9a919ff7 planemo upload for repository https://github.com/Single-Molecule-Genetics/VariantAnalyzerGalaxy/tree/master/tools/variant_analyzer commit ee4a8e6cf290e6c8a4d55f9cd2839d60ab3b11c8-dirty
mheinzl
parents: 75
diff changeset
148 mut_pos_dict[chrom_stop_pos][abba] += 1
0
e5953c54cfb5 planemo upload for repository https://github.com/gpovysil/VariantAnalyzerGalaxy/tree/master/tools/variant_analyzer commit ee4a8e6cf290e6c8a4d55f9cd2839d60ab3b11c8
mheinzl
parents:
diff changeset
149 else:
78
fdfe9a919ff7 planemo upload for repository https://github.com/Single-Molecule-Genetics/VariantAnalyzerGalaxy/tree/master/tools/variant_analyzer commit ee4a8e6cf290e6c8a4d55f9cd2839d60ab3b11c8-dirty
mheinzl
parents: 75
diff changeset
150 mut_pos_dict[chrom_stop_pos][abba] = 1
fdfe9a919ff7 planemo upload for repository https://github.com/Single-Molecule-Genetics/VariantAnalyzerGalaxy/tree/master/tools/variant_analyzer commit ee4a8e6cf290e6c8a4d55f9cd2839d60ab3b11c8-dirty
mheinzl
parents: 75
diff changeset
151 else:
fdfe9a919ff7 planemo upload for repository https://github.com/Single-Molecule-Genetics/VariantAnalyzerGalaxy/tree/master/tools/variant_analyzer commit ee4a8e6cf290e6c8a4d55f9cd2839d60ab3b11c8-dirty
mheinzl
parents: 75
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152 mut_pos_dict[chrom_stop_pos] = {}
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mheinzl
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153 mut_pos_dict[chrom_stop_pos][abba] = 1
fdfe9a919ff7 planemo upload for repository https://github.com/Single-Molecule-Genetics/VariantAnalyzerGalaxy/tree/master/tools/variant_analyzer commit ee4a8e6cf290e6c8a4d55f9cd2839d60ab3b11c8-dirty
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154 if chrom_stop_pos not in ref_pos_dict:
fdfe9a919ff7 planemo upload for repository https://github.com/Single-Molecule-Genetics/VariantAnalyzerGalaxy/tree/master/tools/variant_analyzer commit ee4a8e6cf290e6c8a4d55f9cd2839d60ab3b11c8-dirty
mheinzl
parents: 75
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155 ref_pos_dict[chrom_stop_pos] = {}
fdfe9a919ff7 planemo upload for repository https://github.com/Single-Molecule-Genetics/VariantAnalyzerGalaxy/tree/master/tools/variant_analyzer commit ee4a8e6cf290e6c8a4d55f9cd2839d60ab3b11c8-dirty
mheinzl
parents: 75
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156 ref_pos_dict[chrom_stop_pos][abba] = 0
fdfe9a919ff7 planemo upload for repository https://github.com/Single-Molecule-Genetics/VariantAnalyzerGalaxy/tree/master/tools/variant_analyzer commit ee4a8e6cf290e6c8a4d55f9cd2839d60ab3b11c8-dirty
mheinzl
parents: 75
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157 elif nuc == ref:
fdfe9a919ff7 planemo upload for repository https://github.com/Single-Molecule-Genetics/VariantAnalyzerGalaxy/tree/master/tools/variant_analyzer commit ee4a8e6cf290e6c8a4d55f9cd2839d60ab3b11c8-dirty
mheinzl
parents: 75
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158 count_ref += 1
fdfe9a919ff7 planemo upload for repository https://github.com/Single-Molecule-Genetics/VariantAnalyzerGalaxy/tree/master/tools/variant_analyzer commit ee4a8e6cf290e6c8a4d55f9cd2839d60ab3b11c8-dirty
mheinzl
parents: 75
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159 if chrom_stop_pos in ref_pos_dict:
fdfe9a919ff7 planemo upload for repository https://github.com/Single-Molecule-Genetics/VariantAnalyzerGalaxy/tree/master/tools/variant_analyzer commit ee4a8e6cf290e6c8a4d55f9cd2839d60ab3b11c8-dirty
mheinzl
parents: 75
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160 if abba in ref_pos_dict[chrom_stop_pos]:
fdfe9a919ff7 planemo upload for repository https://github.com/Single-Molecule-Genetics/VariantAnalyzerGalaxy/tree/master/tools/variant_analyzer commit ee4a8e6cf290e6c8a4d55f9cd2839d60ab3b11c8-dirty
mheinzl
parents: 75
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161 ref_pos_dict[chrom_stop_pos][abba] += 1
fdfe9a919ff7 planemo upload for repository https://github.com/Single-Molecule-Genetics/VariantAnalyzerGalaxy/tree/master/tools/variant_analyzer commit ee4a8e6cf290e6c8a4d55f9cd2839d60ab3b11c8-dirty
mheinzl
parents: 75
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162 else:
0
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mheinzl
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163 ref_pos_dict[chrom_stop_pos][abba] = 1
e5953c54cfb5 planemo upload for repository https://github.com/gpovysil/VariantAnalyzerGalaxy/tree/master/tools/variant_analyzer commit ee4a8e6cf290e6c8a4d55f9cd2839d60ab3b11c8
mheinzl
parents:
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164 else:
78
fdfe9a919ff7 planemo upload for repository https://github.com/Single-Molecule-Genetics/VariantAnalyzerGalaxy/tree/master/tools/variant_analyzer commit ee4a8e6cf290e6c8a4d55f9cd2839d60ab3b11c8-dirty
mheinzl
parents: 75
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165 ref_pos_dict[chrom_stop_pos] = {}
fdfe9a919ff7 planemo upload for repository https://github.com/Single-Molecule-Genetics/VariantAnalyzerGalaxy/tree/master/tools/variant_analyzer commit ee4a8e6cf290e6c8a4d55f9cd2839d60ab3b11c8-dirty
mheinzl
parents: 75
diff changeset
166 ref_pos_dict[chrom_stop_pos][abba] = 1
fdfe9a919ff7 planemo upload for repository https://github.com/Single-Molecule-Genetics/VariantAnalyzerGalaxy/tree/master/tools/variant_analyzer commit ee4a8e6cf290e6c8a4d55f9cd2839d60ab3b11c8-dirty
mheinzl
parents: 75
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167 else:
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mheinzl
parents: 75
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168 count_indel += 1
fdfe9a919ff7 planemo upload for repository https://github.com/Single-Molecule-Genetics/VariantAnalyzerGalaxy/tree/master/tools/variant_analyzer commit ee4a8e6cf290e6c8a4d55f9cd2839d60ab3b11c8-dirty
mheinzl
parents: 75
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169 print("coverage at pos %s = %s, ref = %s, alt = %s, other = %s,\n" %
fdfe9a919ff7 planemo upload for repository https://github.com/Single-Molecule-Genetics/VariantAnalyzerGalaxy/tree/master/tools/variant_analyzer commit ee4a8e6cf290e6c8a4d55f9cd2839d60ab3b11c8-dirty
mheinzl
parents: 75
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170 (pileupcolumn.pos, count_ref + count_alt, count_ref, count_alt, count_indel))
0
e5953c54cfb5 planemo upload for repository https://github.com/gpovysil/VariantAnalyzerGalaxy/tree/master/tools/variant_analyzer commit ee4a8e6cf290e6c8a4d55f9cd2839d60ab3b11c8
mheinzl
parents:
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171
78
fdfe9a919ff7 planemo upload for repository https://github.com/Single-Molecule-Genetics/VariantAnalyzerGalaxy/tree/master/tools/variant_analyzer commit ee4a8e6cf290e6c8a4d55f9cd2839d60ab3b11c8-dirty
mheinzl
parents: 75
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172 # if mutation is in DCS file but not in SSCS, then set counts to NA
fdfe9a919ff7 planemo upload for repository https://github.com/Single-Molecule-Genetics/VariantAnalyzerGalaxy/tree/master/tools/variant_analyzer commit ee4a8e6cf290e6c8a4d55f9cd2839d60ab3b11c8-dirty
mheinzl
parents: 75
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173 if chrom_stop_pos not in mut_pos_dict.keys():
fdfe9a919ff7 planemo upload for repository https://github.com/Single-Molecule-Genetics/VariantAnalyzerGalaxy/tree/master/tools/variant_analyzer commit ee4a8e6cf290e6c8a4d55f9cd2839d60ab3b11c8-dirty
mheinzl
parents: 75
diff changeset
174 mut_pos_dict[chrom_stop_pos] = {}
fdfe9a919ff7 planemo upload for repository https://github.com/Single-Molecule-Genetics/VariantAnalyzerGalaxy/tree/master/tools/variant_analyzer commit ee4a8e6cf290e6c8a4d55f9cd2839d60ab3b11c8-dirty
mheinzl
parents: 75
diff changeset
175 mut_pos_dict[chrom_stop_pos]["ab"] = 0
fdfe9a919ff7 planemo upload for repository https://github.com/Single-Molecule-Genetics/VariantAnalyzerGalaxy/tree/master/tools/variant_analyzer commit ee4a8e6cf290e6c8a4d55f9cd2839d60ab3b11c8-dirty
mheinzl
parents: 75
diff changeset
176 mut_pos_dict[chrom_stop_pos]["ba"] = 0
fdfe9a919ff7 planemo upload for repository https://github.com/Single-Molecule-Genetics/VariantAnalyzerGalaxy/tree/master/tools/variant_analyzer commit ee4a8e6cf290e6c8a4d55f9cd2839d60ab3b11c8-dirty
mheinzl
parents: 75
diff changeset
177 ref_pos_dict[chrom_stop_pos] = {}
fdfe9a919ff7 planemo upload for repository https://github.com/Single-Molecule-Genetics/VariantAnalyzerGalaxy/tree/master/tools/variant_analyzer commit ee4a8e6cf290e6c8a4d55f9cd2839d60ab3b11c8-dirty
mheinzl
parents: 75
diff changeset
178 ref_pos_dict[chrom_stop_pos]["ab"] = 0
fdfe9a919ff7 planemo upload for repository https://github.com/Single-Molecule-Genetics/VariantAnalyzerGalaxy/tree/master/tools/variant_analyzer commit ee4a8e6cf290e6c8a4d55f9cd2839d60ab3b11c8-dirty
mheinzl
parents: 75
diff changeset
179 ref_pos_dict[chrom_stop_pos]["ba"] = 0
0
e5953c54cfb5 planemo upload for repository https://github.com/gpovysil/VariantAnalyzerGalaxy/tree/master/tools/variant_analyzer commit ee4a8e6cf290e6c8a4d55f9cd2839d60ab3b11c8
mheinzl
parents:
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180 bam.close()
e5953c54cfb5 planemo upload for repository https://github.com/gpovysil/VariantAnalyzerGalaxy/tree/master/tools/variant_analyzer commit ee4a8e6cf290e6c8a4d55f9cd2839d60ab3b11c8
mheinzl
parents:
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181
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mheinzl
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182 # save counts
e5953c54cfb5 planemo upload for repository https://github.com/gpovysil/VariantAnalyzerGalaxy/tree/master/tools/variant_analyzer commit ee4a8e6cf290e6c8a4d55f9cd2839d60ab3b11c8
mheinzl
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183 with open(sscs_counts_json, "w") as f:
e5953c54cfb5 planemo upload for repository https://github.com/gpovysil/VariantAnalyzerGalaxy/tree/master/tools/variant_analyzer commit ee4a8e6cf290e6c8a4d55f9cd2839d60ab3b11c8
mheinzl
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184 json.dump((mut_pos_dict, ref_pos_dict), f)
e5953c54cfb5 planemo upload for repository https://github.com/gpovysil/VariantAnalyzerGalaxy/tree/master/tools/variant_analyzer commit ee4a8e6cf290e6c8a4d55f9cd2839d60ab3b11c8
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185
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186
e5953c54cfb5 planemo upload for repository https://github.com/gpovysil/VariantAnalyzerGalaxy/tree/master/tools/variant_analyzer commit ee4a8e6cf290e6c8a4d55f9cd2839d60ab3b11c8
mheinzl
parents:
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187 if __name__ == '__main__':
e5953c54cfb5 planemo upload for repository https://github.com/gpovysil/VariantAnalyzerGalaxy/tree/master/tools/variant_analyzer commit ee4a8e6cf290e6c8a4d55f9cd2839d60ab3b11c8
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188 sys.exit(mut2sscs(sys.argv))