Mercurial > repos > mheinzl > variant_analyzer2
diff read2mut.xml @ 55:8fbe6aba07e5 draft
planemo upload for repository https://github.com/Single-Molecule-Genetics/VariantAnalyzerGalaxy/tree/master/tools/variant_analyzer commit ee4a8e6cf290e6c8a4d55f9cd2839d60ab3b11c8
author | mheinzl |
---|---|
date | Fri, 12 Mar 2021 14:18:45 +0000 |
parents | d21960b45a6b |
children | 66c1245436b9 |
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--- a/read2mut.xml Fri Mar 12 08:00:31 2021 +0000 +++ b/read2mut.xml Fri Mar 12 14:18:45 2021 +0000 @@ -1,5 +1,5 @@ <?xml version="1.0" encoding="UTF-8"?> -<tool id="read2mut" name="Call specific mutations in reads:" version="2.1.0" profile="19.01"> +<tool id="read2mut" name="Call specific mutations in reads:" version="2.1.1" profile="19.01"> <description>Looks for reads with mutation at known positions and calculates frequencies and stats.</description> <macros> <import>va_macros.xml</import> @@ -26,6 +26,7 @@ --softclipping_dist '$softclipping_dist' --reads_threshold '$reads_threshold' --outputFile '$output_xlsx' + --outputFile_csv '$outputFile_csv' --outputFile2 '$output_xlsx2' --outputFile3 '$output_xlsx3' ]]> @@ -44,6 +45,7 @@ </inputs> <outputs> <data name="output_xlsx" format="xlsx" label="${tool.name} on ${on_string}: XLSX summary"/> + <data name="outputFile_csv" format="csv" label="${tool.name} on ${on_string}: CSV summary"/> <data name="output_xlsx2" format="xlsx" label="${tool.name} on ${on_string}: XLSX allele frequencies"/> <data name="output_xlsx3" format="xlsx" label="${tool.name} on ${on_string}: XLSX tiers"/> </outputs> @@ -60,6 +62,7 @@ <param name="softclipping_dist" value="15"/> <param name="reads_threshold" value="1.0"/> <output name="output_xlsx" file="Variant_Analyzer_summary_test.xlsx" decompress="true" lines_diff="10"/> + <output name="outputFile_csv" file="Variant_Analyzer_summary_test.csv" decompress="true" lines_diff="10"/> <output name="output_xlsx2" file="Variant_Analyzer_allele_frequencies_test.xlsx" decompress="true" lines_diff="10"/> <output name="output_xlsx3" file="Variant_Analyzer_tiers_test.xlsx" decompress="true" lines_diff="10"/> </test> @@ -75,7 +78,7 @@ **Input** **Dataset 1:** VCF file with duplex consesus sequence (DCS) mutations. E.g. -generated by the `FreeBayes variant caller <https://arxiv.org/abs/1207.3907>`_. +generated by the `FreeBayes <https://arxiv.org/abs/1207.3907>`_ or `LoFreq <https://academic.oup.com/nar/article/40/22/11189/1152727>`_ variant caller. **Dataset 2:** BAM file of aligned raw reads. This file can be obtained by the tool `Map with BWA-MEM <https://arxiv.org/abs/1303.3997>`_. @@ -91,9 +94,10 @@ **Output** The output are three XLSX files containing frequencies stats for DCS mutations based -on information from the raw reads. In addition to that a tier based +on information from the raw reads and a CSV file containing the summary information without color-coding. In addition to that a tier based classification is provided based on the amout of support for a true variant call. + ]]> </help> <expand macro="citation" />