Mercurial > repos > mheinzl > variant_analyzer2
diff mut2read.xml @ 78:fdfe9a919ff7 draft
planemo upload for repository https://github.com/Single-Molecule-Genetics/VariantAnalyzerGalaxy/tree/master/tools/variant_analyzer commit ee4a8e6cf290e6c8a4d55f9cd2839d60ab3b11c8-dirty
author | mheinzl |
---|---|
date | Fri, 22 Jul 2022 09:19:44 +0000 |
parents | 6ccff403db8a |
children | e46d5e377760 |
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--- a/mut2read.xml Mon Mar 29 09:22:57 2021 +0000 +++ b/mut2read.xml Fri Jul 22 09:19:44 2022 +0000 @@ -1,5 +1,5 @@ <?xml version="1.0" encoding="UTF-8"?> -<tool id="mut2read" name="DCS mutations to tags/reads:" version="2.1.1" profile="19.01"> +<tool id="mut2read" name="DCS mutations to tags/reads:" version="3.0.0" profile="19.01"> <description>Extracts all tags that carry a mutation in the duplex consensus sequence (DCS)</description> <macros> <import>va_macros.xml</import> @@ -17,7 +17,7 @@ ]]> </command> <inputs> - <param name="file1" type="data" format="vcf" label="DCS Mutation File" optional="false" help="VCF file with DCS mutations. See Help section below for a detailed explanation."/> + <param name="file1" type="data" format="vcf" label="DCS Mutation File" optional="false" help="VCF file with DCS mutations. See the Help section below for a detailed explanation."/> <param name="file2" type="data" format="bam" label="DCS BAM File" optional="false" help="BAM file with aligned DCS reads."/> <param name="file3" type="data" format="tabular" label="Aligned Families File" optional="false" help="TABULAR file with aligned families."/> </inputs> @@ -27,11 +27,11 @@ </outputs> <tests> <test> - <param name="file1" value="FreeBayes_test.vcf" lines_diff="2"/> + <param name="file1" value="FreeBayes_test.vcf"/> <param name="file2" value="DCS_test.bam"/> <param name="file3" value="Aligned_Families_test.tabular"/> - <output name="output_fastq" file="Interesting_Reads_test.fastq" lines_diff="136"/> - <output name="output_json" file="tag_count_dict_test.json" lines_diff="2"/> + <output name="output_fastq" file="Interesting_Reads_test.fastq"/> + <output name="output_json" file="tag_count_dict_test.json"/> </test> </tests> <help> <![CDATA[ @@ -39,12 +39,12 @@ Takes a VCF file with mutations, a BAM file of aligned DCS reads, and a tabular file with aligned families as input and prints all tags of reads that -carry a mutation to a user specified output file and creates a fastq file of -reads of tags with a mutation. +carry a mutation or have the reference allele to a user-specified output file and creates a fastq file of +reads of tags with a mutation and the reference allele. **Input** -**Dataset 1:** VCF file with duplex consesus sequence (DCS) mutations. E.g. +**Dataset 1:** VCF file with duplex consensus sequence (DCS) mutations. E.g. generated by the `FreeBayes <https://arxiv.org/abs/1207.3907>`_ or `LoFreq <https://academic.oup.com/nar/article/40/22/11189/1152727>`_ variant caller. @@ -57,7 +57,7 @@ **Output** -The output is a json file containing dictonaries of the tags of reads containing mutations +The output is a json file containing dictionaries of the tags of reads containing mutations in the DCS and a fastq file of all reads of these tags. ]]>