Mercurial > repos > mheinzl > variant_analyzer2
diff mut2sscs.xml @ 78:fdfe9a919ff7 draft
planemo upload for repository https://github.com/Single-Molecule-Genetics/VariantAnalyzerGalaxy/tree/master/tools/variant_analyzer commit ee4a8e6cf290e6c8a4d55f9cd2839d60ab3b11c8-dirty
author | mheinzl |
---|---|
date | Fri, 22 Jul 2022 09:19:44 +0000 |
parents | 6ccff403db8a |
children | e46d5e377760 |
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--- a/mut2sscs.xml Mon Mar 29 09:22:57 2021 +0000 +++ b/mut2sscs.xml Fri Jul 22 09:19:44 2022 +0000 @@ -1,6 +1,6 @@ <?xml version="1.0" encoding="UTF-8"?> -<tool id="mut2sscs" name="DCS mutations to SSCS stats:" version="2.1.1" profile="19.01"> - <description>Extracts all tags from the single stranded consensus sequence (SSCS) bam file that carry a mutation at the same position a mutation is called in the duplex consensus sequence (DCS) and calculates their frequencies</description> +<tool id="mut2sscs" name="DCS mutations to SSCS stats:" version="3.0.0" profile="19.01"> + <description>Extracts all tags from the single-stranded consensus sequence (SSCS) bam file that carries a mutation at the same position a mutation is called in the duplex consensus sequence (DCS) and calculates their frequencies</description> <macros> <import>va_macros.xml</import> </macros> @@ -15,7 +15,7 @@ ]]> </command> <inputs> - <param name="file1" type="data" format="vcf" label="DCS Mutation File" optional="false" help="VCF file with DCS mutations. See Help section below for a detailed explanation."/> + <param name="file1" type="data" format="vcf" label="DCS Mutation File" optional="false" help="VCF file with DCS mutations. See the Help section below for a detailed explanation."/> <param name="file2" type="data" format="bam" label="SSCS BAM File" optional="false" help="BAM file with aligned SSCS reads."/> </inputs> <outputs> @@ -25,29 +25,29 @@ <test> <param name="file1" value="FreeBayes_test.vcf"/> <param name="file2" value="SSCS_test.bam"/> - <output name="output_json" file="SSCS_counts_test.json" lines_diff="2"/> + <output name="output_json" file="SSCS_counts_test.json"/> </test> </tests> <help> <![CDATA[ **What it does** Takes a VCF file with DCS mutations and a BAM file of aligned SSCS reads -as input and writes statistics about tags of reads that carry a mutation in the -SSCS at the same position a mutation is called in the DCS to a user specified output file.. +as input and writes statistics about tags of reads that carry a mutation and the reference allele in the +SSCS at the same position a mutation is called in the DCS to a user-specified output file. **Input** -**Dataset 1:** VCF file with duplex consesus sequence (DCS) mutations. E.g. +**Dataset 1:** VCF file with duplex consensus sequence (DCS) mutations. E.g. generated by the `FreeBayes <https://arxiv.org/abs/1207.3907>`_ or `LoFreq <https://academic.oup.com/nar/article/40/22/11189/1152727>`_ variant caller. -**Dataset 2:** BAM file of aligned single stranded consensus sequence (SSCS) +**Dataset 2:** BAM file of the aligned single stranded consensus sequence (SSCS) reads. This file can be obtained by the tool `Map with BWA-MEM <https://arxiv.org/abs/1303.3997>`_. **Output** -The output is a json file containing dictonaries with stats of tags that carry a mutation in the SSCS -at the same position a mutation is called in the DCS. +The output is a json file containing dictionaries with stats of tags that carry a mutation in the SSCS +at the same position, a mutation is called in the DCS. ]]> </help>