Mercurial > repos > miller-lab > genome_diversity
annotate pca.py @ 28:184d14e4270d
Update to Miller Lab devshed revision 4ede22dd5500
| author | Richard Burhans <burhans@bx.psu.edu> |
|---|---|
| date | Wed, 17 Jul 2013 12:46:46 -0400 |
| parents | 8997f2ca8c7a |
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| rev | line source |
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| 0 | 1 #!/usr/bin/env python |
| 2 | |
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3 import gd_util |
| 0 | 4 import os |
|
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5 import re |
| 0 | 6 import shutil |
| 7 import sys | |
| 8 from BeautifulSoup import BeautifulSoup | |
| 9 import gd_composite | |
| 10 | |
| 11 ################################################################################ | |
| 12 | |
| 13 def do_ped2geno(input, output): | |
| 14 lines = [] | |
| 15 with open(input) as fh: | |
| 16 for line in fh: | |
| 17 line = line.rstrip('\r\n') | |
| 18 lines.append(line.split()) | |
| 19 | |
| 20 pair_map = { | |
| 21 '0':{ '0':'9', '1':'9', '2':'9' }, | |
| 22 '1':{ '0':'1', '1':'2', '2':'1' }, | |
| 23 '2':{ '0':'1', '1':'1', '2':'0' } | |
| 24 } | |
| 25 with open(output, 'w') as ofh: | |
| 26 for a_idx in xrange(6, len(lines[0]), 2): | |
| 27 b_idx = a_idx + 1 | |
| 28 print >> ofh, ''.join(map(lambda line: pair_map[line[a_idx]][line[b_idx]], lines)) | |
| 29 | |
| 30 def do_map2snp(input, output): | |
| 31 with open(output, 'w') as ofh: | |
| 32 with open(input) as fh: | |
| 33 for line in fh: | |
| 34 elems = line.split() | |
| 35 print >> ofh, ' {0} 11 0.002 2000 A T'.format(elems[1]) | |
| 36 | |
| 37 def make_ind_file(ind_file, input): | |
| 38 pops = [] | |
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39 name_map = [] |
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40 name_idx = 0 |
| 0 | 41 |
| 42 ofh = open(ind_file, 'w') | |
| 43 | |
| 44 with open(input) as fh: | |
| 45 soup = BeautifulSoup(fh) | |
| 46 misc = soup.find('div', {'id': 'gd_misc'}) | |
| 47 populations = misc('ul')[0] | |
| 48 | |
| 49 i = 0 | |
| 50 for entry in populations: | |
| 51 if i % 2 == 1: | |
| 52 population_name = entry.contents[0].encode('utf8').strip().replace(' ', '_') | |
| 53 pops.append(population_name) | |
| 54 individuals = entry.ol('li') | |
| 55 for individual in individuals: | |
| 56 individual_name = individual.string.encode('utf8').strip() | |
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57 name_map.append(individual_name) |
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58 print >> ofh, 'ind_%s' % name_idx, 'M', population_name |
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59 name_idx += 1 |
| 0 | 60 i += 1 |
| 61 | |
| 62 ofh.close() | |
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63 return pops, name_map |
| 0 | 64 |
| 65 def make_par_file(par_file, geno_file, snp_file, ind_file, evec_file, eval_file): | |
| 66 with open(par_file, 'w') as fh: | |
| 67 print >> fh, 'genotypename: {0}'.format(geno_file) | |
| 68 print >> fh, 'snpname: {0}'.format(snp_file) | |
| 69 print >> fh, 'indivname: {0}'.format(ind_file) | |
| 70 print >> fh, 'evecoutname: {0}'.format(evec_file) | |
| 71 print >> fh, 'evaloutname: {0}'.format(eval_file) | |
| 72 print >> fh, 'altnormstyle: NO' | |
| 73 print >> fh, 'numoutevec: 2' | |
| 74 | |
| 75 def do_smartpca(par_file): | |
| 76 prog = 'smartpca' | |
| 77 | |
| 78 args = [ prog ] | |
| 79 args.append('-p') | |
| 80 args.append(par_file) | |
| 81 | |
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82 stdoutdata, stderrdata = gd_util.run_program(prog, args) |
| 0 | 83 |
| 84 stats = [] | |
| 85 | |
| 86 save_line = False | |
| 87 for line in stdoutdata.split('\n'): | |
| 88 if line.startswith(('## Average divergence', '## Anova statistics', '## Statistical significance')): | |
| 89 stats.append('') | |
| 90 save_line = True | |
| 91 if line.strip() == '': | |
| 92 save_line = False | |
| 93 if save_line: | |
| 94 stats.append(line) | |
| 95 | |
| 96 return '\n'.join(stats[1:]) | |
| 97 | |
| 98 def do_ploteig(evec_file, population_names): | |
| 99 prog = 'gd_ploteig' | |
| 100 | |
| 101 args = [ prog ] | |
| 102 args.append('-i') | |
| 103 args.append(evec_file) | |
| 104 args.append('-c') | |
| 105 args.append('1:2') | |
| 106 args.append('-p') | |
| 107 args.append(':'.join(population_names)) | |
| 108 args.append('-x') | |
| 109 | |
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110 gd_util.run_program(prog, args) |
| 0 | 111 |
| 112 def do_eval2pct(eval_file, explained_file): | |
| 113 prog = 'eval2pct' | |
| 114 | |
| 115 args = [ prog ] | |
| 116 args.append(eval_file) | |
| 117 | |
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118 with open(explained_file, 'w') as fh: |
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119 gd_util.run_program(prog, args, stdout=fh) |
| 0 | 120 |
| 121 def do_coords2admix(coords_file): | |
| 122 prog = 'coords2admix' | |
| 123 | |
| 124 args = [ prog ] | |
| 125 args.append(coords_file) | |
| 126 | |
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127 with open('fake', 'w') as fh: |
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128 gd_util.run_program(prog, args, stdout=fh) |
| 0 | 129 |
| 130 shutil.copy2('fake', coords_file) | |
| 131 | |
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132 ind_regex = re.compile('ind_([0-9]+)') |
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133 |
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134 def fix_names(name_map, files): |
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135 for file in files: |
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136 tmp_filename = '%s.tmp' % file |
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137 with open(tmp_filename, 'w') as ofh: |
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138 with open(file) as fh: |
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139 for line in fh: |
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140 line = line.rstrip('\r\n') |
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141 match = ind_regex.search(line) |
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142 if match: |
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143 idx = int(match.group(1)) |
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144 old = 'ind_%s' % idx |
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145 new = name_map[idx].replace(' ', '_') |
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146 line = line.replace(old, new) |
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147 print >> ofh, line |
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148 |
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149 shutil.copy2(tmp_filename, file) |
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150 os.unlink(tmp_filename) |
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151 |
| 0 | 152 ################################################################################ |
| 153 | |
| 154 if len(sys.argv) != 5: | |
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155 gd_util.die('Usage') |
| 0 | 156 |
| 157 input, input_files_path, output, output_files_path = sys.argv[1:5] | |
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158 gd_util.mkdir_p(output_files_path) |
| 0 | 159 |
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160 ################################################################################ |
| 0 | 161 |
| 162 ped_file = os.path.join(input_files_path, 'admix.ped') | |
| 163 geno_file = os.path.join(output_files_path, 'admix.geno') | |
| 164 do_ped2geno(ped_file, geno_file) | |
| 165 | |
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166 ################################################################################ |
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167 |
| 0 | 168 map_file = os.path.join(input_files_path, 'admix.map') |
| 169 snp_file = os.path.join(output_files_path, 'admix.snp') | |
| 170 do_map2snp(map_file, snp_file) | |
| 171 | |
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172 ################################################################################ |
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173 |
| 0 | 174 ind_file = os.path.join(output_files_path, 'admix.ind') |
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175 population_names, name_map = make_ind_file(ind_file, input) |
| 0 | 176 |
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177 ################################################################################ |
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178 |
| 0 | 179 par_file = os.path.join(output_files_path, 'par.admix') |
| 180 evec_file = os.path.join(output_files_path, 'coordinates.txt') | |
| 181 eval_file = os.path.join(output_files_path, 'admix.eval') | |
| 182 make_par_file(par_file, geno_file, snp_file, ind_file, evec_file, eval_file) | |
| 183 | |
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184 ################################################################################ |
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185 |
| 0 | 186 smartpca_stats = do_smartpca(par_file) |
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187 fix_names(name_map, [ind_file, evec_file]) |
| 0 | 188 |
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189 ################################################################################ |
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190 |
| 0 | 191 do_ploteig(evec_file, population_names) |
| 192 plot_file = 'coordinates.txt.1:2.{0}.pdf'.format(':'.join(population_names)) | |
| 193 output_plot_file = os.path.join(output_files_path, 'PCA.pdf') | |
| 194 shutil.copy2(plot_file, output_plot_file) | |
| 195 os.unlink(plot_file) | |
| 196 | |
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197 ################################################################################ |
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198 |
| 0 | 199 do_eval2pct(eval_file, os.path.join(output_files_path, 'explained.txt')) |
| 200 os.unlink(eval_file) | |
| 201 | |
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202 ################################################################################ |
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203 |
| 0 | 204 do_coords2admix(evec_file) |
| 205 | |
| 206 ################################################################################ | |
| 207 | |
| 208 info_page = gd_composite.InfoPage() | |
| 209 info_page.set_title('PCA Galaxy Composite Dataset') | |
| 210 | |
| 211 display_file = gd_composite.DisplayFile() | |
| 212 display_value = gd_composite.DisplayValue() | |
| 213 | |
| 214 out_pdf = gd_composite.Parameter(name='PCA.pdf', value='PCA.pdf', display_type=display_file) | |
| 215 out_evec = gd_composite.Parameter(name='coordinates.txt', value='coordinates.txt', display_type=display_file) | |
| 216 out_explained = gd_composite.Parameter(name='explained.txt', value='explained.txt', display_type=display_file) | |
| 217 | |
| 218 evec_prefix = 'coordinates.txt.1:2.{0}'.format(':'.join(population_names)) | |
| 219 ps_file = '{0}.ps'.format(evec_prefix) | |
| 220 xtxt_file = '{0}.xtxt'.format(evec_prefix) | |
| 221 | |
| 222 os.unlink(os.path.join(output_files_path, ps_file)) | |
| 223 os.unlink(os.path.join(output_files_path, xtxt_file)) | |
| 224 | |
| 225 info_page.add_output_parameter(out_pdf) | |
| 226 info_page.add_output_parameter(out_evec) | |
| 227 info_page.add_output_parameter(out_explained) | |
| 228 | |
| 229 in_admix = gd_composite.Parameter(name='par.admix', value='par.admix', display_type=display_file) | |
| 230 in_geno = gd_composite.Parameter(name='admix.geno', value='admix.geno', display_type=display_file) | |
| 231 in_snp = gd_composite.Parameter(name='admix.snp', value='admix.snp', display_type=display_file) | |
| 232 in_ind = gd_composite.Parameter(name='admix.ind', value='admix.ind', display_type=display_file) | |
| 233 | |
| 234 info_page.add_input_parameter(in_admix) | |
| 235 info_page.add_input_parameter(in_geno) | |
| 236 info_page.add_input_parameter(in_snp) | |
| 237 info_page.add_input_parameter(in_ind) | |
| 238 | |
| 239 misc_stats = gd_composite.Parameter(description='Stats<p/><pre>\n{0}\n</pre>'.format(smartpca_stats), display_type=display_value) | |
| 240 | |
| 241 info_page.add_misc(misc_stats) | |
| 242 | |
| 243 with open (output, 'w') as ofh: | |
| 244 print >> ofh, info_page.render() | |
| 245 | |
| 246 sys.exit(0) | |
| 247 |