annotate prepare_population_structure.xml @ 18:f04f40a36cc8

Latest changes from Belinda and Cathy. Webb's updates to the Fst tools.
author Richard Burhans <burhans@bx.psu.edu>
date Tue, 23 Oct 2012 12:41:52 -0400
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1 <tool id="gd_prepare_population_structure" name="Prepare Input" version="1.0.0">
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2 <description>: Filter and convert to the format needed for these tools</description>
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3
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4 <command interpreter="python">
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5 prepare_population_structure.py "$input" "$min_reads" "$min_qual" "$min_spacing" "$output" "$output.files_path"
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6 #if $individuals.choice == '0'
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7 "all_individuals"
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8 #else if $individuals.choice == '1'
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9 #for $population in $individuals.populations
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10 #set $pop_arg = 'population:%s:%s' % (str($population.p_input), str($population.p_input.name))
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11 "$pop_arg"
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12 #end for
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13 #end if
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14 #for $individual, $individual_col in zip($input.dataset.metadata.individual_names, $input.dataset.metadata.individual_columns)
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15 #set $arg = 'individual:%s:%s' % ($individual_col, $individual)
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16 "$arg"
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17 #end for
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18 </command>
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19
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20 <inputs>
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21 <param name="input" type="data" format="gd_snp" label="SNP dataset" />
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22 <conditional name="individuals">
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23 <param name="choice" type="select" label="Individuals">
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24 <option value="0" selected="true">All individuals</option>
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25 <option value="1">Specified populations</option>
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26 </param>
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27 <when value="0" />
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28 <when value="1">
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29 <repeat name="populations" title="Population" min="1">
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30 <param name="p_input" type="data" format="gd_indivs" label="Individuals" />
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31 </repeat>
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32 </when>
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33 </conditional>
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34 <param name="min_reads" type="integer" min="0" value="0" label="Minimum SNP coverage" />
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35 <param name="min_qual" type="integer" min="0" value="0" label="Minimum SNP quality" />
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36 <param name="min_spacing" type="integer" min="0" value="0" label="Minimum spacing between SNPs" />
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37 </inputs>
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38
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39 <outputs>
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40 <data name="output" format="gd_ped">
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41 <actions>
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42 <action type="metadata" name="base_name" default="admix" />
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43 </actions>
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44 </data>
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45 </outputs>
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46
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47 <tests>
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48 <test>
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49 <param name="input" value="test_in/sample.gd_snp" ftype="gd_snp" />
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50 <param name="min_reads" value="3" />
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51 <param name="min_qual" value="30" />
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52 <param name="min_spacing" value="0" />
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53 <param name="choice" value="0" />
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54 <output name="output" file="test_out/prepare_population_structure/prepare_population_structure.html" ftype="html" compare="diff" lines_diff="2">
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55 <extra_files type="file" name="admix.map" value="test_out/prepare_population_structure/admix.map" />
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56 <extra_files type="file" name="admix.ped" value="test_out/prepare_population_structure/admix.ped" />
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57 </output>
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58 </test>
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59 </tests>
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60
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61 <help>
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62
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63 **Dataset formats**
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64
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65 The input datasets are in gd_snp_ and gd_indivs_ formats.
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66 The output dataset is in gd_ped_ format. (`Dataset missing?`_)
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67
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68 .. _gd_snp: ./static/formatHelp.html#gd_snp
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69 .. _gd_indivs: ./static/formatHelp.html#gd_indivs
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70 .. _gd_ped: ./static/formatHelp.html#gd_ped
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71 .. _Dataset missing?: ./static/formatHelp.html
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72
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73 -----
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74
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75 **What it does**
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76
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77 This tool converts a gd_snp dataset into the format needed for estimating
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78 the population structure. You can select the individuals to be included,
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79 by using "population" datasets created via the Specify Individuals tool.
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80 (It is important for these population datasets to have distinguishable names,
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81 since they will be stored in the output's metadata so that subsequent tools
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82 can use them as labels. If necessary, rename the datasets to give them
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83 distinct and meaningful names before running this tool.)
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84
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85 You can also filter the SNPs, based on criteria such as minimum coverage
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86 (a qualifying SNP must have at least this many reads for every included
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87 individual), minimum quality score (for every included individual), and/or
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88 minimum spacing (SNPs that are too close together on the same chromosome or
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89 scaffold are discarded). In addition to producing the filtered and formatted
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90 .map and .ped files for subsequent analysis, the tool reports the number of
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91 SNPs meeting these conditions, which can be seen by clicking on the eye icon
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92 in the history panel after the program runs.
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93
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94 -----
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95
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96 **Example**
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97
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98 - input::
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99
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100 Contig161_chr1_4641264_4641879 115 C T 73.5 chr1 4641382 C 6 0 2 45 8 0 2 51 15 0 2 72 5 0 2 42 6 0 2 45 10 0 2 57 Y 54 0.323 0
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101 Contig48_chr1_10150253_10151311 11 A G 94.3 chr1 10150264 A 1 0 2 30 1 0 2 30 1 0 2 30 3 0 2 36 1 0 2 30 1 0 2 30 Y 22 +99. 0
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102 Contig20_chr1_21313469_21313570 66 C T 54.0 chr1 21313534 C 4 0 2 39 4 0 2 39 5 0 2 42 4 0 2 39 4 0 2 39 5 0 2 42 N 1 +99. 0
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103 etc.
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104
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105 - output cover page::
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106
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107 Prepare to look for population structure Galaxy Composite Dataset
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108 Output completed: 2012-10-01 04:09:36 PM
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109
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110 Outputs
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111 * admix.ped (link)
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112 * admix.map (link)
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113 * Using 222 of 400 SNPs
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114
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115 Inputs
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116 * Minimum reads covering a SNP, per individual: 6
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117 * Minimum quality value, per individual: 0
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118 * Minimum spacing between SNPs on the same scaffold: 0
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119
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120 Populations
f04f40a36cc8 Latest changes from Belinda and Cathy. Webb's updates to the Fst tools.
Richard Burhans <burhans@bx.psu.edu>
parents: 14
diff changeset
121 * Pop. A
f04f40a36cc8 Latest changes from Belinda and Cathy. Webb's updates to the Fst tools.
Richard Burhans <burhans@bx.psu.edu>
parents: 14
diff changeset
122 1. PB1
f04f40a36cc8 Latest changes from Belinda and Cathy. Webb's updates to the Fst tools.
Richard Burhans <burhans@bx.psu.edu>
parents: 14
diff changeset
123 2. PB2
f04f40a36cc8 Latest changes from Belinda and Cathy. Webb's updates to the Fst tools.
Richard Burhans <burhans@bx.psu.edu>
parents: 14
diff changeset
124 * Pop. B
f04f40a36cc8 Latest changes from Belinda and Cathy. Webb's updates to the Fst tools.
Richard Burhans <burhans@bx.psu.edu>
parents: 14
diff changeset
125 1. PB3
f04f40a36cc8 Latest changes from Belinda and Cathy. Webb's updates to the Fst tools.
Richard Burhans <burhans@bx.psu.edu>
parents: 14
diff changeset
126 2. PB4
f04f40a36cc8 Latest changes from Belinda and Cathy. Webb's updates to the Fst tools.
Richard Burhans <burhans@bx.psu.edu>
parents: 14
diff changeset
127 * Pop. C
f04f40a36cc8 Latest changes from Belinda and Cathy. Webb's updates to the Fst tools.
Richard Burhans <burhans@bx.psu.edu>
parents: 14
diff changeset
128 1. PB6
f04f40a36cc8 Latest changes from Belinda and Cathy. Webb's updates to the Fst tools.
Richard Burhans <burhans@bx.psu.edu>
parents: 14
diff changeset
129 2. PB8
14
8ae67e9fb6ff Uploaded Miller Lab Devshed version a51c894f5bed again [possible toolshed.g2 bug]
miller-lab
parents:
diff changeset
130
8ae67e9fb6ff Uploaded Miller Lab Devshed version a51c894f5bed again [possible toolshed.g2 bug]
miller-lab
parents:
diff changeset
131 </help>
8ae67e9fb6ff Uploaded Miller Lab Devshed version a51c894f5bed again [possible toolshed.g2 bug]
miller-lab
parents:
diff changeset
132 </tool>