Mercurial > repos > miller-lab > genome_diversity
annotate prepare_population_structure.xml @ 18:f04f40a36cc8
Latest changes from Belinda and Cathy. Webb's updates to the Fst tools.
author | Richard Burhans <burhans@bx.psu.edu> |
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date | Tue, 23 Oct 2012 12:41:52 -0400 |
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children | 248b06e86022 |
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1 <tool id="gd_prepare_population_structure" name="Prepare Input" version="1.0.0"> |
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2 <description>: Filter and convert to the format needed for these tools</description> |
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3 |
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4 <command interpreter="python"> |
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5 prepare_population_structure.py "$input" "$min_reads" "$min_qual" "$min_spacing" "$output" "$output.files_path" |
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6 #if $individuals.choice == '0' |
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7 "all_individuals" |
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8 #else if $individuals.choice == '1' |
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9 #for $population in $individuals.populations |
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10 #set $pop_arg = 'population:%s:%s' % (str($population.p_input), str($population.p_input.name)) |
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11 "$pop_arg" |
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12 #end for |
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13 #end if |
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14 #for $individual, $individual_col in zip($input.dataset.metadata.individual_names, $input.dataset.metadata.individual_columns) |
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15 #set $arg = 'individual:%s:%s' % ($individual_col, $individual) |
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16 "$arg" |
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17 #end for |
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18 </command> |
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19 |
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20 <inputs> |
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21 <param name="input" type="data" format="gd_snp" label="SNP dataset" /> |
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22 <conditional name="individuals"> |
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23 <param name="choice" type="select" label="Individuals"> |
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24 <option value="0" selected="true">All individuals</option> |
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25 <option value="1">Specified populations</option> |
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26 </param> |
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27 <when value="0" /> |
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28 <when value="1"> |
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29 <repeat name="populations" title="Population" min="1"> |
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30 <param name="p_input" type="data" format="gd_indivs" label="Individuals" /> |
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31 </repeat> |
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32 </when> |
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33 </conditional> |
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34 <param name="min_reads" type="integer" min="0" value="0" label="Minimum SNP coverage" /> |
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35 <param name="min_qual" type="integer" min="0" value="0" label="Minimum SNP quality" /> |
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36 <param name="min_spacing" type="integer" min="0" value="0" label="Minimum spacing between SNPs" /> |
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37 </inputs> |
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38 |
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39 <outputs> |
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40 <data name="output" format="gd_ped"> |
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41 <actions> |
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42 <action type="metadata" name="base_name" default="admix" /> |
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43 </actions> |
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44 </data> |
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45 </outputs> |
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46 |
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47 <tests> |
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48 <test> |
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49 <param name="input" value="test_in/sample.gd_snp" ftype="gd_snp" /> |
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50 <param name="min_reads" value="3" /> |
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51 <param name="min_qual" value="30" /> |
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52 <param name="min_spacing" value="0" /> |
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53 <param name="choice" value="0" /> |
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54 <output name="output" file="test_out/prepare_population_structure/prepare_population_structure.html" ftype="html" compare="diff" lines_diff="2"> |
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55 <extra_files type="file" name="admix.map" value="test_out/prepare_population_structure/admix.map" /> |
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56 <extra_files type="file" name="admix.ped" value="test_out/prepare_population_structure/admix.ped" /> |
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57 </output> |
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58 </test> |
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59 </tests> |
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60 |
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61 <help> |
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62 |
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63 **Dataset formats** |
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64 |
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65 The input datasets are in gd_snp_ and gd_indivs_ formats. |
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66 The output dataset is in gd_ped_ format. (`Dataset missing?`_) |
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67 |
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68 .. _gd_snp: ./static/formatHelp.html#gd_snp |
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69 .. _gd_indivs: ./static/formatHelp.html#gd_indivs |
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70 .. _gd_ped: ./static/formatHelp.html#gd_ped |
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71 .. _Dataset missing?: ./static/formatHelp.html |
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72 |
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73 ----- |
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74 |
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75 **What it does** |
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76 |
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77 This tool converts a gd_snp dataset into the format needed for estimating |
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78 the population structure. You can select the individuals to be included, |
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79 by using "population" datasets created via the Specify Individuals tool. |
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80 (It is important for these population datasets to have distinguishable names, |
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81 since they will be stored in the output's metadata so that subsequent tools |
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82 can use them as labels. If necessary, rename the datasets to give them |
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83 distinct and meaningful names before running this tool.) |
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84 |
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85 You can also filter the SNPs, based on criteria such as minimum coverage |
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86 (a qualifying SNP must have at least this many reads for every included |
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87 individual), minimum quality score (for every included individual), and/or |
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88 minimum spacing (SNPs that are too close together on the same chromosome or |
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89 scaffold are discarded). In addition to producing the filtered and formatted |
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90 .map and .ped files for subsequent analysis, the tool reports the number of |
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91 SNPs meeting these conditions, which can be seen by clicking on the eye icon |
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92 in the history panel after the program runs. |
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93 |
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94 ----- |
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95 |
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96 **Example** |
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97 |
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98 - input:: |
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99 |
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100 Contig161_chr1_4641264_4641879 115 C T 73.5 chr1 4641382 C 6 0 2 45 8 0 2 51 15 0 2 72 5 0 2 42 6 0 2 45 10 0 2 57 Y 54 0.323 0 |
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101 Contig48_chr1_10150253_10151311 11 A G 94.3 chr1 10150264 A 1 0 2 30 1 0 2 30 1 0 2 30 3 0 2 36 1 0 2 30 1 0 2 30 Y 22 +99. 0 |
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102 Contig20_chr1_21313469_21313570 66 C T 54.0 chr1 21313534 C 4 0 2 39 4 0 2 39 5 0 2 42 4 0 2 39 4 0 2 39 5 0 2 42 N 1 +99. 0 |
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103 etc. |
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104 |
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105 - output cover page:: |
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106 |
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107 Prepare to look for population structure Galaxy Composite Dataset |
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108 Output completed: 2012-10-01 04:09:36 PM |
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109 |
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110 Outputs |
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111 * admix.ped (link) |
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112 * admix.map (link) |
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113 * Using 222 of 400 SNPs |
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114 |
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115 Inputs |
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116 * Minimum reads covering a SNP, per individual: 6 |
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117 * Minimum quality value, per individual: 0 |
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118 * Minimum spacing between SNPs on the same scaffold: 0 |
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119 |
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120 Populations |
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121 * Pop. A |
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122 1. PB1 |
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123 2. PB2 |
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124 * Pop. B |
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125 1. PB3 |
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126 2. PB4 |
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127 * Pop. C |
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128 1. PB6 |
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129 2. PB8 |
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130 |
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131 </help> |
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132 </tool> |