Mercurial > repos > miller-lab > genome_diversity
diff extract_flanking_dna.xml @ 21:d6b961721037
Miller Lab Devshed version 4c04e35b18f6
author | Richard Burhans <burhans@bx.psu.edu> |
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date | Mon, 05 Nov 2012 12:44:17 -0500 |
parents | 8ae67e9fb6ff |
children |
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--- a/extract_flanking_dna.xml Tue Oct 23 14:38:04 2012 -0400 +++ b/extract_flanking_dna.xml Mon Nov 05 12:44:17 2012 -0500 @@ -12,13 +12,13 @@ </command> <inputs> - <param format="tabular" name="input" type="data" label="Selected SNPS dataset"/> - <param name="output_format" type="select" format="integer" label="output format"> + <param format="tabular" name="input" type="data" label="SNP dataset"/> + <param name="output_format" type="select" format="integer" label="Output format"> <option value="fasta" selected="true">FastA format</option> - <option value="primer3">Primer3 input</option> + <option value="primer3">Boulder-IO (for Primer3)</option> </param> <conditional name="override_metadata"> - <param name="choice" type="select" format="integer" label="choose columns"> + <param name="choice" type="select" format="integer" label="Choose columns" help="Datasets in gd_snp format have the columns in the metadata, all others need the columns chosen." > <option value="0" selected="true">No, get columns from metadata</option> <option value="1" >Yes, choose columns</option> </param> @@ -53,17 +53,31 @@ <help> +**Dataset formats** + +The input dataset is in tabular_ format and must contain a scaffold or +chromosome column and a position column. The output is in fasta_ format or +Boulder-IO_ format used by Primer3. +(`Dataset missing?`_) + +.. _tabular: ./static/formatHelp.html#tab +.. _fasta: ./static/formatHelp.html#fasta +.. _Boulder-IO: ./static/formatHelp.html#boulder +.. _Dataset missing?: ./static/formatHelp.html + +----- + **What it does** - This tool reports a DNA segment containing each SNP, with up to 200 nucleotides on - either side of the SNP position, which is indicated by "n". Fewer nucleotides - are reported if the SNP is near an end of the assembled genome fragment. +This tool reports a DNA segment containing each SNP, with up to 200 nucleotides +on either side of the SNP position, which is indicated by "n". Fewer nucleotides +are reported if the SNP is near an end of the assembled genome fragment. ----- **Example** -- input file:: +- input (gd_snp format):: chr2_75111355_75112576 314 A C L F chr2 75111676 C F 15 4 53 2 9 48 Y 96 0.369 0.355 0.396 0 chr8_93901796_93905612 2471 A C A A chr8 93904264 A A 8 0 51 10 2 14 Y 961 0.016 0.534 0.114 2 @@ -77,7 +91,7 @@ chr19_39866997_39874915 3117 C T P P chr19 39870110 C P 3 7 65 14 2 32 Y 6 0.321 0.911 0.462 4 etc. -- output file:: +- output (FastA format):: > chr2_75111355_75112576 314 A C TATCTTCATTTTTATTATAGACTCTCTGAACCAATTTGCCCTGAGGCAGACTTTTTAAAGTACTGTGTAATGTATGAAGTCCTTCTGCTCAAGCAAATCATTGGCATGAAAACAGTTGCAAACTTATTGTGAGAGAAGAGTCCAAGAGTTTTAACAGTCTGTAAGTATATAGCCTGTGAGTTTGATTTCCTTCTTGTTTTTnTTCCAGAAACATGATCAGGGGCAAGTTCTATTGGATATAGTCTTCAAGCATCTTGATTTGACTGAGCGTGACTATTTTGGTTTGCAGTTGACTGACGATTCCACTGATAACCCAGTAAGTTTAAGCTGTTGTCTTTCATTGTCATTGCAATTTTTCTGTCTTTATACTAGGTCCTTTCTGATTTACATTGTTCACTGATT