Mercurial > repos > miller-lab > genome_diversity
view phylogenetic_tree.py @ 28:184d14e4270d
Update to Miller Lab devshed revision 4ede22dd5500
author | Richard Burhans <burhans@bx.psu.edu> |
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date | Wed, 17 Jul 2013 12:46:46 -0400 |
parents | 8997f2ca8c7a |
children |
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#!/usr/bin/env python import gd_util import os import sys from Population import Population import gd_composite ################################################################################ if len(sys.argv) != 12: gd_util.die('Usage') input, output, extra_files_path, input_type, data_source_arg, minimum_coverage, minimum_quality, p1_input, dbkey, draw_tree_options, ind_arg = sys.argv[1:] if input_type == 'gd_snp': if data_source_arg == 'sequence_coverage': data_source = 0 elif data_source_arg == 'estimated_genotype': data_source = 1 else: gd_util.die('Unsupported data_source: {0}'.format(data_source_arg)) elif input_type == 'gd_genotype': data_source = 1 minimum_coverage = 0 minimum_quality = 0 else: gd_util.die('Unsupported input_type:: {0}'.format(input_type)) # note: TEST THIS if dbkey in ['', '?', 'None']: dbkey = 'none' p_total = Population() p_total.from_wrapped_dict(ind_arg) if p1_input == "all_individuals": tags = p_total.tag_list() else: p1 = Population() p1.from_population_file(p1_input) if not p_total.is_superset(p1): gd_util.die('There is an individual in the population that is not in the SNP table') tags = p1.tag_list() ################################################################################ gd_util.mkdir_p(extra_files_path) phylip_outfile = os.path.join(extra_files_path, 'distance_matrix.phylip') newick_outfile = os.path.join(extra_files_path, 'phylogenetic_tree.newick') ps_outfile = 'tree.ps' pdf_outfile = os.path.join(extra_files_path, 'tree.pdf') informative_snp_file = os.path.join(extra_files_path, 'informative_snps.txt') mega_distance_matrix_file = os.path.join(extra_files_path, 'mega_distance_matrix.txt') ################################################################################ prog = 'dist_mat' args = [ prog ] args.append(input) args.append(minimum_coverage) args.append(minimum_quality) args.append(dbkey) args.append(data_source) args.append(informative_snp_file) args.append(mega_distance_matrix_file) for tag in tags: if input_type == 'gd_genotype': column, name = tag.split(':') tag = '{0}:{1}'.format(int(column) - 2, name) args.append(tag) with open(phylip_outfile, 'w') as fh: gd_util.run_program(prog, args, stdout=fh) ################################################################################ prog = 'quicktree' args = [ prog ] args.append('-in') args.append('m') args.append('-out') args.append('t') args.append(phylip_outfile) with open(newick_outfile, 'w') as fh: gd_util.run_program(prog, args, stdout=fh) ################################################################################ prog = 'draw_tree' args = [ prog ] if draw_tree_options: args.append(draw_tree_options) args.append(newick_outfile) with open(ps_outfile, 'w') as fh: gd_util.run_program(prog, args, stdout=fh) ################################################################################ prog = 'ps2pdf' args = [ prog ] args.append('-dPDFSETTINGS=/prepress') args.append(ps_outfile) args.append(pdf_outfile) gd_util.run_program(prog, args) ################################################################################ info_page = gd_composite.InfoPage() info_page.set_title('Phylogenetic tree Galaxy Composite Dataset') display_file = gd_composite.DisplayFile() display_value = gd_composite.DisplayValue() out_pdf = gd_composite.Parameter(name='tree.pdf', value='tree.pdf', display_type=display_file) out_newick = gd_composite.Parameter(value='phylogenetic_tree.newick', name='phylogenetic tree (newick)', display_type=display_file) out_phylip = gd_composite.Parameter(value='distance_matrix.phylip', name='Phylip distance matrix', display_type=display_file) out_mega = gd_composite.Parameter(value='mega_distance_matrix.txt', name='Mega distance matrix', display_type=display_file) out_snps = gd_composite.Parameter(value='informative_snps.txt', name='informative SNPs', display_type=display_file) info_page.add_output_parameter(out_pdf) info_page.add_output_parameter(out_newick) info_page.add_output_parameter(out_phylip) info_page.add_output_parameter(out_mega) info_page.add_output_parameter(out_snps) in_min_cov = gd_composite.Parameter(description='Minimum coverage', value=minimum_coverage, display_type=display_value) in_min_qual = gd_composite.Parameter(description='Minimum quality', value=minimum_quality, display_type=display_value) include_ref_value = 'no' if dbkey != 'none': include_ref_value = 'yes' in_include_ref = gd_composite.Parameter(description='Include reference sequence', value=include_ref_value, display_type=display_value) if data_source == 0: data_source_value = 'sequence coverage' elif data_source == 1: data_source_value = 'estimated genotype' in_data_source = gd_composite.Parameter(description='Data source', value=data_source_value, display_type=display_value) branch_type_value = 'square' if 'd' in draw_tree_options: branch_type_value = 'diagonal' in_branch_type = gd_composite.Parameter(description='Branch type', value=branch_type_value, display_type=display_value) branch_scale_value = 'yes' if 's' in draw_tree_options: branch_scale_value = 'no' in_branch_scale = gd_composite.Parameter(description='Draw branches to scale', value=branch_scale_value, display_type=display_value) branch_length_value = 'yes' if 'b' in draw_tree_options: branch_length_value = 'no' in_branch_length = gd_composite.Parameter(description='Show branch lengths', value=branch_length_value, display_type=display_value) tree_layout_value = 'horizontal' if 'v' in draw_tree_options: tree_layout_value = 'vertical' in_tree_layout = gd_composite.Parameter(description='Tree layout', value=tree_layout_value, display_type=display_value) info_page.add_input_parameter(in_min_cov) info_page.add_input_parameter(in_min_qual) info_page.add_input_parameter(in_include_ref) info_page.add_input_parameter(in_data_source) info_page.add_input_parameter(in_branch_type) info_page.add_input_parameter(in_branch_scale) info_page.add_input_parameter(in_branch_length) info_page.add_input_parameter(in_tree_layout) misc_individuals = gd_composite.Parameter(name='Individuals', value=tags, display_type=gd_composite.DisplayTagList()) info_page.add_misc(misc_individuals) with open(output, 'w') as ofh: print >> ofh, info_page.render() ################################################################################ sys.exit(0)