Mercurial > repos > miller-lab > genome_diversity
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author | Richard Burhans <burhans@bx.psu.edu> |
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date | Wed, 20 Jun 2012 14:02:30 -0400 |
parents | e29f4d801bb0 |
children | 9b92372de9f6 |
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<tool id="gd_modify_snp_table" name="Modify" version="1.0.0"> <description>a SNP table</description> <command interpreter="python"> modify_snp_table.py "$input" "$p1_input" "$output" #if $limit_coverage.choice == "0" "-1" "-1" "-1" "-1" #else "${limit_coverage.lo_coverage}" "${limit_coverage.hi_coverage}" "${limit_coverage.low_ind_cov}" "${limit_coverage.lo_quality}" #end if #for $individual, $individual_col in zip($input.dataset.metadata.individual_names, $input.dataset.metadata.individual_columns) #set $arg = '%s:%s' % ($individual_col, $individual) "$arg" #end for </command> <inputs> <param name="input" type="data" format="snp" label="SNP table" /> <param name="p1_input" type="data" format="ind" label="Population individuals" /> <conditional name="limit_coverage"> <param name="choice" type="select" format="integer" label="Option"> <option value="0" selected="true">add columns to the SNP table</option> <option value="1">discard some SNPs</option> </param> <when value="0" /> <when value="1"> <param name="lo_coverage" type="integer" min="0" value="0" label="Lower bound on total coverage" /> <param name="hi_coverage" type="integer" min="0" value="1000" label="Upper bound on total coverage" /> <param name="low_ind_cov" type="integer" min="0" value="0" label="Lower bound on individual coverage" /> <param name="lo_quality" type="integer" min="0" value="0" label="Lower bound on individual quality values" /> </when> </conditional> </inputs> <outputs> <data name="output" format="snp" metadata_source="input" /> </outputs> <tests> <test> <param name="input" value="test_in/sample.snp" ftype="snp" /> <param name="p1_input" value="test_in/a.ind" ftype="ind" /> <param name="choice" value="1" /> <param name="lo_coverage" value="0" /> <param name="hi_coverage" value="1000" /> <param name="low_ind_cov" value="3" /> <param name="lo_quality" value="30" /> <output name="output" file="test_out/modify_snp_table/modify.snp" /> </test> </tests> <help> **What it does** The user specifies that some of the individuals in the selected SNP table are form a "population" that has been previously defined using the Galaxy tool to select individuals from a SNP table. One option is for the program to append four columns to the table, giving the total counts for the two alleles, the "genotype" for the population and the maximum quality value, taken over all individuals in the population. If all defined genotypes in the population are 2 (agree with the reference), the population's genotype is 2; similarly for 0; otherwise the genotype is 1 (unless all individuals have undefined genotype, in which case it is -1. The other option is to remove rows from the table for which the total coverage for the population is either too low or too high, and/or if the individual coverage or quality value is too low. </help> </tool>