Mercurial > repos > mingchen0919 > aurora_fastqc_site
view 08_sequence_length_distribution.Rmd @ 0:b7c115edd970 draft
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author | mingchen0919 |
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date | Tue, 27 Feb 2018 10:37:12 -0500 |
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children | c64267b9f754 |
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--- output: html_document --- ```{r setup, include=FALSE, warning=FALSE, message=FALSE} knitr::opts_chunk$set( echo = as.logical(opt$X_e), error = TRUE, eval = TRUE ) ``` # Sequence Length Distribution ```{r 'Sequence Length Distribution', fig.width=10} ## reads 1 sld_1 = extract_data_module(paste0(opt$X_d, '/read_1_fastqc/fastqc_data.txt'), 'Sequence Length Distribution') sld_1$id = 1:length(sld_1$X.Length) sld_1$trim = 'before' ## reads 2 sld_2 = extract_data_module(paste0(opt$X_d, '/read_2_fastqc/fastqc_data.txt'), 'Sequence Length Distribution') sld_2$id = 1:length(sld_2$X.Length) sld_2$trim = 'after' comb_sld = rbind(sld_1, sld_2) comb_sld$trim = factor(levels = c('before', 'after'), comb_sld$trim) p = ggplot(data = comb_sld, aes(x = id, y = Count)) + geom_line(color = 'red') + scale_x_continuous(breaks = sld_2$id, labels = sld_2$X.Length) + facet_grid(. ~ trim) + xlab('Sequence Length (bp)') + ylab('') + theme(axis.text.x = element_text(angle=45)) ggplotly(p) ```