Mercurial > repos > mingchen0919 > rmarkdown_deseq2
annotate DESeq.xml @ 9:924d122d0027 draft default tip
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author | mingchen0919 |
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date | Thu, 16 Nov 2017 10:16:29 -0500 |
parents | 5f7665898fd9 |
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rev | line source |
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7 | 1 <tool id="DESeq" name="DESeq2: DESeq" version="2.0.0"> |
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planemo upload for repository https://github.com/statonlab/docker-GRReport/tree/master/my_tools/rmarkdown_deseq2 commit 9285c2b8ad41a486dde2a87600a6b8267841c8b5-dirty
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2 <requirements> |
5
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3 <requirement type="package" version="1.15.0.6-0">pandoc</requirement> |
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4 <requirement type="package" version="1.20.0">r-getopt</requirement> |
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5 <requirement type="package" version="1.2">r-rmarkdown</requirement> |
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6 <requirement type="package" version="1.8.4">r-plyr</requirement> |
6 | 7 <requirement type="package" version="1.14.1">bioconductor-deseq2</requirement> |
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8 <requirement type="package" version="1.1.0">r-stringr</requirement> |
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9 <requirement type="package" version="0.4.0">r-highcharter</requirement> |
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10 <requirement type="package" version="0.2">r-dt</requirement> |
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11 <requirement type="package" version="1.4.2">r-reshape2</requirement> |
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12 <requirement type="package" version="4.5.6">r-plotly</requirement> |
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13 <requirement type="package" version="0.2.0.1">r-formattable</requirement> |
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14 <requirement type="package" version="0.3.5">r-htmltools</requirement> |
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15 <requirement type="package" version="1.0.8">r-pheatmap</requirement> |
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16 </requirements> |
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17 <description> |
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18 An R Markdown tool to perform DESeq analysis. |
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19 </description> |
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20 <stdio> |
6 | 21 <!--redirecting stderr to a file. "XXX" is used to match with nothing so that tool running won't be interrupted during testing--> |
22 <regex match="XXX" | |
23 source="stderr" | |
24 level="warning" | |
25 description="Check the warnings_and_errors.txt file for more details."/> | |
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26 </stdio> |
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27 <command> |
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28 <![CDATA[ |
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29 |
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30 Rscript '${__tool_directory__}/DESeq_render.R' |
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31 |
6 | 32 |
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33 ## 1. input data |
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34 -e $echo |
6 | 35 ##----- code chunk to get file paths and raw file names for a multiple inputs data field ---- |
36 #set $sep = '' | |
37 #set $count_file_paths = '' | |
38 #set $count_file_names = '' | |
39 #for $count_file in $count_files: | |
40 #set $count_file_paths += $sep + str($count_file) | |
41 #set $count_file_names += $sep + str($count_file.name) | |
42 #set $sep = ',' | |
43 #end for | |
44 ##----------------- end for getting file names and file paths ------------------------------ | |
45 -P '$count_file_paths' | |
46 -N '$count_file_names' | |
47 -S $sample_table | |
48 -p '$design_formula' | |
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49 |
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50 ## 2. output report and report site directory |
6 | 51 -r $report |
52 -d $report.files_path | |
53 -s $sink_message | |
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54 -w $deseq_workspace |
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55 |
6 | 56 ## 3. Rmd templates from the tool directory |
57 -t '${__tool_directory__}/DESeq.Rmd' | |
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58 |
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59 |
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60 |
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61 ]]> |
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62 </command> |
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63 <inputs> |
6 | 64 <param type="boolean" name="echo" truevalue="TRUE" falsevalue="FALSE" checked="false" |
65 label="Display analysis code in report?"/> | |
9 | 66 <param type="data" name="count_files" format="txt" multiple="true" label="Count files from htseq-count"/> |
67 <param type="data" name="sample_table" format="txt" multiple="false" label="sample table file" | |
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68 help="The sample table file contains a table. The first column is the sample name, the second column is |
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69 the count file name and the rest of columns are treatment columns. The file names in this table have |
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70 to be in the same order as the count files uploaded in the previous step. "/> |
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71 <param type="text" name="design_formula" value="~ condition_1 + condition_2" label="Design formula" |
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72 help="The simplest design formula for differential expression would be ~ condition, where condition |
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73 is a column in colData(dds) that specifies which of two (or more groups) the samples belong to"> |
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74 <sanitizer> |
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75 <valid initial="default"> |
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76 <add preset="string.printable"/> |
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77 <add value="~"/> |
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78 </valid> |
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79 </sanitizer> |
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80 </param> |
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81 </inputs> |
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82 <outputs> |
6 | 83 <data name="report" format="html" label="DESeq Analysis on ${on_string}"/> |
84 <data format="txt" name="sink_message" label="Warnings and Errors on ${on_string}" from_work_dir="warnings_and_errors.txt"/> | |
85 <data name="deseq_workspace" format="rdata" label="R workspace: DESeq analysis on ${on_string}"/> | |
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86 </outputs> |
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87 <citations> |
8 | 88 <citation type="bibtex"> |
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89 @article{love2014moderated, |
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90 title={Moderated estimation of fold change and dispersion for RNA-seq data with DESeq2}, |
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91 author={Love, Michael I and Huber, Wolfgang and Anders, Simon}, |
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92 journal={Genome biology}, |
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93 volume={15}, |
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94 number={12}, |
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95 pages={550}, |
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96 year={2014}, |
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97 publisher={BioMed Central} |
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98 } |
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99 </citation> |
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100 <citation type="bibtex"> |
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101 @article{allaire2016rmarkdown, |
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102 title={rmarkdown: Dynamic Documents for R, 2016}, |
6 | 103 author={Allaire, J and Cheng, Joe and Xie, Yihui and McPherson, Jonathan and Chang, Winston and Allen, Jeff |
104 and Wickham, Hadley and Atkins, Aron and Hyndman, Rob}, | |
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105 journal={R package version 0.9}, |
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106 volume={6}, |
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107 year={2016} |
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108 } |
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109 </citation> |
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110 <citation type="bibtex"> |
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111 @book{xie2015dynamic, |
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112 title={Dynamic Documents with R and knitr}, |
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113 author={Xie, Yihui}, |
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114 volume={29}, |
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115 year={2015}, |
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116 publisher={CRC Press} |
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117 } |
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118 </citation> |
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119 </citations> |
0
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120 </tool> |