Mercurial > repos > mingchen0919 > rmarkdown_fastqc_site
view 06_per_sequence_gc_content.Rmd @ 11:507eec497730 draft
update fastqc site
author | mingchen0919 |
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date | Tue, 07 Nov 2017 16:52:24 -0500 |
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--- title: 'Per sequence GC content' output: html_document: number_sections: true toc: true theme: cosmo highlight: tango --- ```{r setup, include=FALSE, warning=FALSE, message=FALSE} knitr::opts_chunk$set( echo = ECHO, error = TRUE ) ``` ### Per sequence GC content ```{r 'Per sequence GC content', fig.width=10} ## reads 1 psGCc_1 = extract_data_module('REPORT_DIR/reads_1_fastqc_data.txt', 'Per sequence GC content') psGCc_1$trim = 'before' ## reads 2 psGCc_2 = extract_data_module('REPORT_DIR/reads_2_fastqc_data.txt', 'Per sequence GC content') psGCc_2$trim = 'after' comb_psGCc = rbind(psGCc_1, psGCc_2) comb_psGCc$trim = factor(levels = c('before', 'after'), comb_psGCc$trim) p = ggplot(data = comb_psGCc, aes(x = X.GC.Content, y = Count)) + geom_line(color = 'red') + facet_grid(. ~ trim) + xlab('Mean Sequence Qaulity (Phred Score)') + ylab('') ggplotly(p) ```