--- /dev/null	Thu Jan 01 00:00:00 1970 +0000
+++ b/wgcna_eigengene_visualization_render.R	Tue Aug 08 12:35:50 2017 -0400
@@ -0,0 +1,109 @@
+##======= Handle arguments from command line ========
+# setup R error handline to go to stderr
+options(show.error.messages=FALSE,
+        error=function(){
+          cat(geterrmessage(), file=stderr())
+          quit("no", 1, F)
+        })
+
+# we need that to not crash galaxy with an UTF8 error on German LC settings.
+loc = Sys.setlocale("LC_MESSAGES", "en_US.UTF-8")
+
+# suppress warning
+options(warn = -1)
+
+options(stringsAsFactors=FALSE, useFancyQuotes=FALSE)
+args = commandArgs(trailingOnly=TRUE)
+
+suppressPackageStartupMessages({
+  library(getopt)
+  library(tools)
+})
+
+# column 1: the long flag name
+# column 2: the short flag alias. A SINGLE character string
+# column 3: argument mask
+#           0: no argument
+#           1: argument required
+#           2: argument is optional
+# column 4: date type to which the flag's argument shall be cast.
+#           possible values: logical, integer, double, complex, character.
+spec_list=list()
+
+##------- 1. input data ---------------------
+spec_list$ECHO = c('echo', 'e', '1', 'character')
+spec_list$CONSTRUCT_NETWORK_WORKSPACE = c('construct_network_workspace', 'w', '1', 'character')
+spec_list$SOFT_THRESHOLD_POWER = c('soft_threshold_power', 'p', '2', 'double')
+spec_list$PLOT_GENES = c('plot_genes', 'n', '1', 'integer')
+
+
+##--------2. output report and report site directory --------------
+spec_list$OUTPUT_HTML = c('wgcna_eigengene_visualization_html', 'o', '1', 'character')
+spec_list$OUTPUT_DIR = c('wgcna_eigengene_visualization_dir', 'd', '1', 'character')
+
+
+
+##--------3. Rmd templates in the tool directory ----------
+
+spec_list$WGCNA_EIGENGENE_VISUALIZATION_RMD = c('wgcna_eigengene_visualization_rmd', 'M', '1', 'character')
+
+
+
+##------------------------------------------------------------------
+
+spec = t(as.data.frame(spec_list))
+opt = getopt(spec)
+# arguments are accessed by long flag name (the first column in the spec matrix)
+#                        NOT by element name in the spec_list
+# example: opt$help, opt$expression_file
+##====== End of arguments handling ==========
+
+#------ Load libraries ---------
+library(rmarkdown)
+library(WGCNA)
+library(DT)
+library(htmltools)
+library(ggplot2)
+
+
+#----- 1. create the report directory ------------------------
+system(paste0('mkdir -p ', opt$wgcna_eigengene_visualization_dir))
+
+
+#----- 2. generate Rmd files with Rmd templates --------------
+#   a. templates without placeholder variables:
+#         copy templates from tool directory to the working directory.
+#   b. templates with placeholder variables:
+#         substitute variables with user input values and place them in the working directory.
+
+
+#----- 01 wgcna_eigengene_visualization.Rmd -----------------------
+readLines(opt$wgcna_eigengene_visualization_rmd) %>%
+  (function(x) {
+    gsub('ECHO', opt$echo, x)
+  }) %>%
+  (function(x) {
+    gsub('CONSTRUCT_NETWORK_WORKSPACE', opt$construct_network_workspace, x)
+  }) %>%
+  (function(x) {
+    gsub('SOFT_THRESHOLD_POWER', opt$soft_threshold_power, x)
+  }) %>%
+  (function(x) {
+    gsub('PLOT_GENES', opt$plot_genes, x)
+  }) %>%
+  (function(x) {
+    gsub('OUTPUT_DIR', opt$wgcna_eigengene_visualization_dir, x)
+  }) %>%
+  (function(x) {
+    fileConn = file('wgcna_eigengene_visualization.Rmd')
+    writeLines(x, con=fileConn)
+    close(fileConn)
+  })
+
+
+#------ 3. render all Rmd files --------
+render('wgcna_eigengene_visualization.Rmd', output_file = opt$wgcna_eigengene_visualization_html)
+
+#-------4. manipulate outputs -----------------------------
+
+