annotate libexec/callSomaticVariants.pl @ 17:8260a4188b08

solved conf_file.ini pb
author mini
date Wed, 01 Oct 2014 13:45:33 +0200
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1 #!/usr/bin/env perl
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2
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3 =head1 LICENSE
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4
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5 Strelka Workflow Software
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6 Copyright (c) 2009-2013 Illumina, Inc.
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7
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8 This software is provided under the terms and conditions of the
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9 Illumina Open Source Software License 1.
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10
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11 You should have received a copy of the Illumina Open Source
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12 Software License 1 along with this program. If not, see
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13 <https://github.com/downloads/sequencing/licenses/>.
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14
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15 =head1 SYNOPSIS
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16
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17 callSomaticVariants.pl [options] | --help
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18
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19 =head2 SUMMARY
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20
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21 Run the somatic variant caller for snvs and indels on a single
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22 chromosome bin.
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23
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24 =cut
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25
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26 use warnings FATAL => 'all';
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27 use strict;
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28
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29 use Carp;
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30 $SIG{__DIE__} = \&Carp::confess;
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31
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32 use File::Spec;
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33 use Getopt::Long;
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34 use Pod::Usage;
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35
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36 my $baseDir;
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37 my $libDir;
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38 BEGIN {
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39
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40 my $thisDir=(File::Spec->splitpath($0))[1];
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41 $baseDir=File::Spec->catdir($thisDir,File::Spec->updir());
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42 $libDir=File::Spec->catdir($baseDir,'lib');
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43 }
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44 use lib $libDir;
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45 use Utils;
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46 print "all imported call";
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47
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48 if(getAbsPath($baseDir)) {
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49 errorX("Can't resolve path for strelka_workflow install directory: '$baseDir'");
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50 }
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51 my $libexecDir=File::Spec->catdir($baseDir,'libexec');
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52
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53
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54 my $scriptName=(File::Spec->splitpath($0))[2];
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55 my $argCount=scalar(@ARGV);
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56 my $cmdline = join(' ',$0,@ARGV);
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57
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58
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59 my ($chrom, $binId, $configFile);
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60 my $help;
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61
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62 GetOptions(
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63 "chrom=s" => \$chrom,
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64 "bin=s" => \$binId,
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65 "config=s" => \$configFile,
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66 "help|h" => \$help) or pod2usage(2);
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67
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68 pod2usage(2) if($help);
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69 pod2usage(2) unless(defined($chrom));
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70 pod2usage(2) unless(defined($binId));
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71 pod2usage(2) unless(defined($configFile));
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72
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73
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74
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75 #
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76 # check all fixed paths (not based on commandline arguments):
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77 #
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78 checkDir($baseDir);
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79 checkDir($libexecDir);
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80
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81 my $strelkaBin=File::Spec->catdir($libexecDir,'strelka2');
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82 checkFile($strelkaBin,"strelka binary");
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83
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84
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85
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86 #
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87 # read config and validate values
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88 #
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89 checkFile($configFile,"configuration ini");
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90 my $config = parseConfigIni($configFile);
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91
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92 for (qw(knownGenomeSize tumorBam normalBam refFile outDir)) {
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93 errorX("Undefined configuration option: '$_'") unless(defined($config->{derived}{$_}));
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94 }
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95
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96 # we skip maxInputDepth,minTier2Mapq here to allow older config files
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97 for (qw(minTier1Mapq isWriteRealignedBam binSize ssnvPrior sindelPrior
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98 ssnvNoise sindelNoise ssnvNoiseStrandBiasFrac)) {
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99 errorX("Undefined configuration option: '$_'") unless(defined($config->{user}{$_}));
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100 }
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101
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102 my $outDir = $config->{derived}{outDir};
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103 my $binDir = File::Spec->catdir($outDir,'chromosomes',$chrom,'bins',$binId);
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104 checkDir($outDir,"output");
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105 checkDir($binDir,"output bin");
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106
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107
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108 my $tumorBam = $config->{derived}{tumorBam};
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109 my $normalBam = $config->{derived}{normalBam};
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110 my $refFile = $config->{derived}{refFile};
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111 checkFile($tumorBam,"tumor BAM");
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112 checkFile($normalBam,"normal BAM");
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113 checkFile($refFile,"reference");
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114
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115
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116 # pull out some config options for convenience:
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117 my $binSize=$config->{user}{binSize};
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118 my $isWriteRealignedBam=$config->{user}{isWriteRealignedBam};
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119 my $knownGenomeSize = $config->{derived}{knownGenomeSize};
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120
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121
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122 my $begin = (int($binId)*$binSize)+1;
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123 my $end = ((int($binId)+1)*$binSize);
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124 #my $end = $begin+100000; #debug mode
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125
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126 my $useroptions = $config->{user};
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127
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128 # set previous default value if an older config file is being used:
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129 if(! defined($useroptions->{minTier2Mapq})) {
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130 $useroptions->{minTier2Mapq} = 5;
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131 }
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132
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133
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134 #
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135 # setup the strelka command-line:
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136 #
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137 my $strelka_base_opts= "-clobber" .
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138 " -filter-unanchored" .
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139 " -min-paired-align-score " . $useroptions->{minTier1Mapq} .
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140 " -min-single-align-score 10" .
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141 " -min-qscore 0" .
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142 " -report-range-begin $begin -report-range-end $end" .
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143 " -samtools-reference '$refFile'" .
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144 " -max-window-mismatch 3 20 -print-used-allele-counts" .
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145 " -bam-seq-name '" . $chrom . "'" .
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146 " -genome-size $knownGenomeSize" .
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147 " -max-indel-size 50" .
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148 " -indel-nonsite-match-prob 0.5" .
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149 " --min-contig-open-end-support 35" .
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150 " --somatic-snv-rate " . $useroptions->{ssnvPrior} .
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151 " --shared-site-error-rate " . $useroptions->{ssnvNoise} .
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152 " --shared-site-error-strand-bias-fraction " . $useroptions->{ssnvNoiseStrandBiasFrac} .
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153 " --somatic-indel-rate " . $useroptions->{sindelPrior} .
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154 " --shared-indel-error-rate " . $useroptions->{sindelNoise} .
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155 " --tier2-min-single-align-score " . $useroptions->{minTier2Mapq} .
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156 " --tier2-min-paired-align-score " . $useroptions->{minTier2Mapq} .
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157 " --tier2-single-align-score-rescue-mode" .
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158 " --tier2-mismatch-density-filter-count 10" .
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159 " --tier2-no-filter-unanchored" .
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160 " --tier2-indel-nonsite-match-prob 0.25" .
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161 " --tier2-include-singleton" .
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162 " --tier2-include-anomalous";
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163
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164
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165 my $somSnvFile='somatic.snvs.unfiltered.vcf';
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166 my $somIndelFile='somatic.indels.unfiltered.vcf';
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167
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168 my $cmd = "$strelkaBin $strelka_base_opts" .
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169 " -bam-file " . $normalBam .
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170 " --tumor-bam-file " . $tumorBam .
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171 " --somatic-snv-file " . File::Spec->catfile($binDir,$somSnvFile) .
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172 " --somatic-indel-file " . File::Spec->catfile($binDir,$somIndelFile) .
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173 " --variant-window-flank-file 50 " . File::Spec->catfile($binDir,$somIndelFile . '.window');
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174
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175
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176 sub ualignFile($) {
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177 return File::Spec->catfile($binDir,$_[0] . ".unsorted.realigned.bam");
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178 }
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179 sub alignFile($) {
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180 return File::Spec->catfile($binDir,$_[0] . ".realigned");
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181 }
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182
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183
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184 if(exists($useroptions->{maxInputDepth}) && ($useroptions->{maxInputDepth} > 0)) {
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185 $cmd .= " --max-input-depth " . $useroptions->{maxInputDepth};
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186 }
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187
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188
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189 if($isWriteRealignedBam) {
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190 $cmd .= " -realigned-read-file " . ualignFile("normal") .
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191 " --tumor-realigned-read-file " . ualignFile("tumor");
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192 }
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193
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194 if(defined($useroptions->{extraStrelkaArguments})){
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195 my $arg=$useroptions->{extraStrelkaArguments};
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196 if($arg !~ /^\s*$/) {
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197 $cmd .= " " . $arg;
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198 }
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199 }
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200
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201 # this file contains site stats that used to be printed on stdout:
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202 #
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203 $cmd .=
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204 " --report-file " . File::Spec->catfile($binDir,'strelka.stats');
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205
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206 $cmd .=
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207 " >| " . File::Spec->catfile($binDir,'strelka.stdout') .
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208 " 2>| " . File::Spec->catfile($binDir,'strelka.stderr');
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209
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210
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211 executeCmd($cmd,0);
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212
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213
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214 if($isWriteRealignedBam) {
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215 for my $label (qw(normal tumor)) {
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216 my $ufile = ualignFile($label);
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217 if( -f $ufile ) {
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218 my $afile = alignFile($label);
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219 my $cmd = "samtools sort " . $ufile . " " . $afile;
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220 executeCmd($cmd,0);
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221 unlink($ufile);
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222 } else {
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223 logX("Can't find unsorted realigned BAM file: '$ufile'");
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224 }
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225 }
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226 }
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227
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228
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229 1;
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230
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231 __END__
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232
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233