Mercurial > repos > mini > strelka
diff strelka2/strelka2.xml @ 0:7a9f20ca4ad5
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author | mini |
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date | Thu, 25 Sep 2014 11:59:08 -0400 |
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--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/strelka2/strelka2.xml Thu Sep 25 11:59:08 2014 -0400 @@ -0,0 +1,126 @@ +<tool id="strelka88" name="Strelka good interface but no dependencies"> + + <description>Strelka good interface but no dependencies</description> + <command interpreter="python">strelka_wrapper.py --tumorBam $tumorBam --normalBam $normalBam --refFile $refFile + #if $configuration.configuration_switch == 'Default': + --configFile Default + #else if $configuration.configuration_switch == 'Path': + --configFile $configuration.configFile + #else: + --configFile Custom + --depthFilterMultiple $configuration.depthFilterMultiple + --snvMaxFilteredBasecallFrac $configuration.snvMaxFilteredBasecallFrac + --snvMaxSpanningDeletionFrac $configuration.snvMaxSpanningDeletionFrac + --indelMaxRefRepeat $configuration.indelMaxRefRepeat + --indelMaxWindowFilteredBasecallFrac $configuration.indelMaxWindowFilteredBasecallFrac + --indelMaxIntHpolLength $configuration.indelMaxIntHpolLength + --ssnvPrior $configuration.ssnvPrior + --sindelPrior $configuration.sindelPrior + --ssnvNoise $configuration.ssnvNoise + --sindelNoise $configuration.sindelNoise + --ssnvNoiseStrandBiasFrac $configuration.ssnvNoiseStrandBiasFrac + --minTier1Mapq $configuration.minTier1Mapq + --minTier2Mapq $configuration.minTier2Mapq + --ssnvQuality_LowerBound $configuration.ssnvQuality_LowerBound + --sindelQuality_LowerBound $configuration.sindelQuality_LowerBound + --isWriteRealignedBam $configuration.isWriteRealignedBam + --binSize $configuration.binSize + --isSkipDepthFilters $configuration.isSkipDepthFilters + --maxInputDepth $configuration.maxInputDepth + #if $configuration.extra_arguments.extra_arguments_switch == 'Yes': + --extraStrelkaArguments $configuration.extra_arguments.extraStrelkaArguments + #end if + #end if + </command> + + <inputs> + <param format="bam" name="tumorBam" type="data" label="Tumor bam file"/> + <param format="bam" name="normalBam" type="data" label="Normal bam file"/> + <param format="fasta" name="refFile" type="data" label="ref fasta file"/> + + <conditional name="configuration"> + <param name="configuration_switch" type="select" label="how do you want to configure strelka"> + <option value="Default" selected="true">Default</option> + <option value="Path">Use a config file</option> + <option value="Custom">Custom</option> + </param> + <when value="Default"> + <!-- do nothing --> + </when> + <when value="Path"> + <param format="ini" name="configFile" type="data" label="config file"/> + </when> + <when value="Custom"> + <param name="binSize" type="integer" value="25000000" label="binSize" /> + <param name="minTier1Mapq" type="integer" value="20" min="0" max="40" help="between 0 and 40" label="minTier1Mapq" /> + <param name="minTier2Mapq" type="integer" value="5" min="0" max="5" help="between 0 and 5" label="minTier2Mapq" /> + <param name="isWriteRealignedBam" type="integer" value="0" label="isWriteRealignedBam" /> + <param name="ssnvPrior" type="float" value="0.000001" label="ssnvPrior" /> + <param name="sindelPrior" type="float" value="0.000001" label="sindelPrior" /> + <param name="ssnvNoise" type="float" value="0.0000005" label="ssnvNoise" /> + <param name="sindelNoise" type="float" value="0.000001" label="sindelNoise" /> + <param name="ssnvNoiseStrandBiasFrac" type="float" value="0.5" label="ssnvNoiseStrandBiasFrac" /> + <param name="ssnvQuality_LowerBound" type="integer" value="15" label="ssnvQuality_LowerBound" /> + <param name="sindelQuality_LowerBound" type="integer" value="30" label="sindelQuality_LowerBound" /> + <param name="isSkipDepthFilters" type="integer" value="1" label="isSkipDepthFilters" /> + <param name="depthFilterMultiple" type="float" value="3.0" label="depthFilterMultiple" /> + <param name="snvMaxFilteredBasecallFrac" type="float" value="0.4" label="snvMaxFilteredBasecallFrac" /> + <param name="snvMaxSpanningDeletionFrac" type="float" value="0.75" label="snvMaxSpanningDeletionFrac" /> + <param name="indelMaxRefRepeat" type="integer" value="8" label="indelMaxRefRepeat" /> + <param name="indelMaxWindowFilteredBasecallFrac" type="float" value="0.3" label="indelMaxWindowFilteredBasecallFrac" /> + <param name="indelMaxIntHpolLength" type="integer" value="14" label="indelMaxIntHpolLength" /> + <param name="maxInputDepth" type="integer" value="10000" label="maxInputDepth" /> + <conditional name="extra_arguments"> + <param name="extra_arguments_switch" type="select" label="Do you Want to add extraStrelkaArguments?"> + <option value="No" selected="true">No</option> + <option value="Yes">Yes</option> + </param> + <when value="No"> + <!-- do nothing --> + </when> + <when value="Yes"> + <param name="extraStrelkaArguments" type="text" value="" label="extraStrelkaArguments" /> + </when> + </conditional> + </when> + </conditional> + <conditional name="conf_file"> + <param name="conf_file_switch" type="select" label="output conf_file ?"> + <option value="No" selected="true">No</option> + <option value="Yes">Yes</option> + </param> + </conditional> + + + + + </inputs> + + <outputs> + <data format="vcf" name="output1_vcf" label="${tool.name} on ${on_string}(passed.somatic.snvs.vcf)" from_work_dir="StrelkaAnalysis/results/passed.somatic.snvs.vcf"/> + <data format="vcf" name="output2_vcf" label="${tool.name} on ${on_string}(passed.somatic.indels.vcf)" from_work_dir="StrelkaAnalysis/results/passed.somatic.indels.vcf"/> + <data format="vcf" name="output3_vcf" label="${tool.name} on ${on_string}(all.somatic.snvs.vcf)" from_work_dir="StrelkaAnalysis/results/all.somatic.snvs.vcf"/> + <data format="vcf" name="output4_vcf" label="${tool.name} on ${on_string}(all.somatic.indels.vcf)" from_work_dir="StrelkaAnalysis/results/all.somatic.indels.vcf"/> + <data name="conf_file.ini" label="conf_file.ini" from_work_dir="StrelkaAnalysis/tmp/config.ini"> + <filter>conf_file.conf_file_switch == "Yes"</filter> + </data> + </outputs> + + <tests> + <test> + <param name="normalBam" ftype="bam" value="NA12891_dupmark_chr20_region.bam"/> + <param name="tumorBam" ftype="bam" value="NA12892_dupmark_chr20_region.bam"/> + <param name="refFile" ftype="fasta" value="chr20_860k_only.fa"/> + <param name="configuration_switch" value="Default"/> + <output name="output1_vcf" file="passed.somatic.snvs.vcf"/> + <output name="output2_vcf" file="passed.somatic.indels.vcf"/> + <output name="output3_vcf" file="all.somatic.snvs.vcf"/> + <output name="output4_vcf" file="all.somatic.indels.vcf"/> + </test> + </tests> + + <help> +Strelka, a method for somatic SNV and small indel detectipon from sequencing data of matched tumor-normal samples. + </help> + +</tool>