Mercurial > repos > modencode-dcc > bamedit
view bamedit.py @ 15:eb166cebbe3c draft default tip
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author | modencode-dcc |
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date | Fri, 25 Jan 2013 11:49:17 -0500 |
parents | 1ceeba7b95a4 |
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#purpose: python wrapper for bamedit tool #author: Ziru Zhou #date: October, 2012 import sys, subprocess, tempfile, shutil, glob, os, os.path, gzip from galaxy import eggs import pkg_resources pkg_resources.require( "simplejson" ) import simplejson CHUNK_SIZE = 1024 def main(): options = simplejson.load( open( sys.argv[1] ) ) script_path = sys.argv[2] #experiment_name = '_'.join( options['bamout'] ) if(options['action'] == "merge"): cmdline = "samtools merge %s %s %s" % ( options['bamout'], options['input1'], options['input2'] ) if('input3' in options): cmdline = "samtools merge %s %s %s %s" % ( options['bamout'], options['input1'], options['input2'], options['input3'] ) elif (options['action'] == "split"): cmdline = "bash %s/split.sh %s %s %s" % ( script_path, options['bamout'], options['bamout2'], options['input1'] ) elif (options['action'] == "pileup"): cmdline = "perl %s/pileup.pl %s %s %s %s %s" % ( script_path, options['input1'], options['input2'], options['bamout'], options['bamname'], options['refname'] ) elif (options['action'] == "filter"): cmdline = "samtools view -q %s %s -bo %s" % ( options['quality'], options['input1'], options['bamout'] ) #create tempdir for output files and stderr reports tmp_dir = tempfile.mkdtemp() stderr_name = tempfile.NamedTemporaryFile().name proc = subprocess.Popen( args=cmdline, shell=True, cwd=tmp_dir, stderr=open( stderr_name, 'wb' ) ) proc.wait() #Do not terminate if error code, allow dataset (e.g. log) creation and cleanup if proc.returncode: stderr_f = open( stderr_name ) while True: chunk = stderr_f.read( CHUNK_SIZE ) if not chunk: stderr_f.close() break sys.stderr.write( chunk ) if __name__ == "__main__": main()