Mercurial > repos > modencode-dcc > idr_package
diff batch-consistency-plot.r @ 4:bdd073e7ad8b draft
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author | modencode-dcc |
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date | Thu, 17 Jan 2013 15:45:33 -0500 |
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--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/batch-consistency-plot.r Thu Jan 17 15:45:33 2013 -0500 @@ -0,0 +1,68 @@ +# 1-20-10 Qunhua Li +# +# This program first plots correspondence curve and IDR threshold plot +# (i.e. number of selected peaks vs IDR) for each pair of sample +# +# usage: +# Rscript batch-consistency-plot-merged.r [npairs] [output.dir] [input.file.prefix 1, 2, 3 ...] +# [npairs]: integer, number of consistency analyses +# (e.g. if 2 replicates, npairs=1, if 3 replicates, npairs=3 +# [output.prefix]: output directory and file name prefix for plot eg. /plots/idrPlot +# [input.file.prefix 1, 2, 3]: prefix for the output from batch-consistency-analysis2. They are the input files for merged analysis see below for examples (i.e. saved.file.prefix). It can be multiple files +# + +args <- commandArgs(trailingOnly=T) +npair <- args[1] # number of curves to plot on the same figure +output.file.prefix <- args[2] # file name for plot, generated from script at the outer level +df.txt <- 10 +ntemp <- as.numeric(npair) +saved.file.prefix <- list() # identifier of filenames that contain the em and URI results +source("/mnt/galaxyTools/galaxy-central/tools/modENCODE_DCC_tools/idr/functions-all-clayton-12-13.r") + +uri.list <- list() +uri.list.match <- list() +ez.list <- list() +legend.txt <- c() +em.output.list <- list() +uri.output.list <- list() + +for(i in 1:npair){ + saved.file.prefix[i] <- args[2+i] + + load(paste(saved.file.prefix[i], "-uri.sav", sep="")) + load(paste(saved.file.prefix[i], "-em.sav", sep="")) + + uri.output.list[[i]] <- uri.output + em.output.list[[i]] <- em.output + + ez.list[[i]] <- get.ez.tt.all(em.output, uri.output.list[[i]]$data12.enrich$merge1, + uri.output.list[[i]]$data12.enrich$merge2) # reverse =T for error rate + + # URI for all peaks + uri.list[[i]] <- uri.output$uri.n + # URI for matched peaks + uri.match <- get.uri.matched(em.output$data.pruned, df=df.txt) + uri.list.match[[i]] <- uri.match$uri.n + + file.name <- unlist(strsplit(as.character(saved.file.prefix[i]), "/")) + + legend.txt[i] <- paste(i, "=", file.name[length(file.name)]) + +} + +plot.uri.file <- paste(output.file.prefix, "-plot.ps", sep="") + +############# plot and report output +# plot correspondence curve for each pair, +# plot number of selected peaks vs IDR +# plot all into 1 file +postscript(paste(output.file.prefix, "-plot.ps", sep="")) +par(mfcol=c(2,3), mar=c(5,6,4,2)+0.1) +plot.uri.group(uri.list, NULL, file.name=NULL, c(1:npair), title.txt="all peaks") +plot.uri.group(uri.list.match, NULL, file.name=NULL, c(1:npair), title.txt="matched peaks") +plot.ez.group(ez.list, plot.dir=NULL, file.name=NULL, legend.txt=c(1:npair), y.lim=c(0, 0.6)) +plot(0, 1, type="n", xlim=c(0,1), ylim=c(0,1), xlab="", ylab="", xaxt="n", yaxt="n") # legends +legend(0, 1, legend.txt, cex=0.6) + +dev.off() +