Mercurial > repos > modencode-dcc > macs
changeset 3:72be1ab49f77 draft
Uploaded
author | modencode-dcc |
---|---|
date | Fri, 18 Jan 2013 11:18:11 -0500 |
parents | 8f4f3945dea7 |
children | 211849e90dd4 |
files | macs2npk.sh |
diffstat | 1 files changed, 60 insertions(+), 0 deletions(-) [+] |
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--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/macs2npk.sh Fri Jan 18 11:18:11 2013 -0500 @@ -0,0 +1,60 @@ +#!/bin/bash +# Converts macs xls output to narrowPeak output + +# Command Usage: macs2npk.sh INPUTFILE OUTPUTFILE + +if [[ "$#" -lt 1 ]] + then + echo $(basename $0) 1>&2 + echo "Converts MACS peak caller xls output file to narrowPeak format" 1>&2 + echo "USAGE:" 1>&2 + echo "$(basename $0) <MACSXlsFile> <outputDir>" 1>&2 + exit 1 +fi + +MACSFILE=$1 +if [[ ! -e ${MACSFILE} ]] + then + echo "MACS xls file ${MACSFILE} does not exist" 1>&2 + exit 1 +fi +# ODIR=$(dirname ${MACSFILE}) +# [[ $# -gt 1 ]] && ODIR=$2 +# if [[ ! -d ${ODIR} ]] +# then +# echo "Output directory ${ODIR} does not exist" 1>&2 +# exit 1 +# fi + +# OFILE="${ODIR}/$(echo $(basename ${MACSFILE} '_peaks.xls')).regionPeak.gz" +OFILE="${2}" + +# XLS format +# chr start stop length summit tags -10log10(pvalue) fold_enrichment %FDR + +# narrowPeak format +# chr start stop name score strand signalValue -log10(pValue) -log10(qValue) summit + +# Remove comments # +# Remove empty lines +# Remove header +# Sort by p-value and then rearrange columns +# adjust start coordinates + +# Check if header has FDR column +header=$(sed -r -e '/^#/d' -e '/^$/d' "${MACSFILE}" | head -1) +hasFdr=0 +echo ${header} | grep -q 'FDR' && hasFDR=1 + +if [[ ${hasFDR} -eq 1 ]] + then + sed -r -e '/^#/d' -e '/^$/d' "${MACSFILE}" | \ + sed 1d | \ + sort -k7nr,7nr | \ + awk '$2 < 1 {$2=1} {printf "%s\t%d\t%d\t%d\t%s\t.\t%s\t%s\t%f\t%d\n",$1,$2-1,$3,NR,$6,$8,$7/10,-log(($9+1e-30)/100)/log(10),$5}' > ${OFILE} +else + sed -r -e '/^#/d' -e '/^$/d' "${MACSFILE}" | \ + sed 1d | \ + sort -k7nr,7nr | \ + awk '$2 < 1 {$2=1} {printf "%s\t%d\t%d\t%d\t%s\t.\t%s\t%s\t-1\t%d\n",$1,$2-1,$3,NR,$6,$8,$7/10,$5}' > ${OFILE} +fi