comparison goseq.xml @ 0:fe71b97cc1a5 draft

planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/goseq_1_22_0 commit 85e0f34aec3045f48ca9b1a00db02de199a99c95-dirty
author mvdbeek
date Wed, 24 Feb 2016 15:54:37 -0500
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1 <tool id="goseq" name="goseq gene ontology analyser" version="0.1.1">
2 <description />
3 <requirements>
4 <requirement type="package" version="3.2.1">R</requirement>
5 <requirement type="package" version="1.22.0">goseq</requirement>
6 </requirements>
7 <command interpreter="Rscript">
8 goseq.r --dge_file "$dge_file"
9 --p_adj_column "$p_adj_column"
10 --cutoff "$p_adj_cutoff"
11 --genome "$genome"
12 --gene_id "$gene_id"
13 --wallenius_tab "$wallenius_tab"
14 --sampling_tab "$sampling_tab"
15 --nobias_tab "$nobias_tab"
16 --length_bias_plot "$length_bias_plot"
17 --sample_vs_wallenius_plot "$sample_vs_wallenius_plot"
18 --repcnt "$repcnt"
19 </command>
20 <inputs>
21 <param help="deseq2/edger/limma differential gene expression list" label="DGE list" name="dge_file" type="data" format="tabular" />
22 <param help="Select the column that contains the multiple-testing corrected p-value" label="p adjust column" name="p_adj_column" type="data_column" data_ref="dge_file"/>
23 <param help="Typically 0.05 after multiple testing correction" max="1" label="Minimum p adjust value to consider gene differentially expressed" name="p_adj_cutoff" type="float" value="0.05" />
24 <param help="Needed to retrive gene length for length correction" label="Select the genome source" name="genome" size="3" type="select">
25 <options from_file="genomes.loc">
26 <column name="value" index="0"/>
27 <column name="name" index="1"/>
28 </options>
29 </param>
30 <param help="Needed for GO analysis" label="Select gene identifier" name="gene_id" type="select">
31 <options from_file="gene_ids.loc">
32 <column name="value" index="0"/>
33 <column name="name" index="1"/>
34 </options>
35 </param>
36 <param help="Do this many random samplings. Larger values take a long time" label="Number of random sampling" name="repcnt" size="3" type="integer" min="100" max="10000" value="1000" />
37 </inputs>
38 <outputs>
39 <data format="tabular" label="Ranked category list - wallenius" name="wallenius_tab" />
40 <data format="tabular" label="Ranked category list - sampling" name="sampling_tab" />
41 <data format="tabular" label="Ranked category list - no length bias correction" name="nobias_tab" />
42 <data format="pdf" label="length bias plot" name="length_bias_plot" />
43 <data format="pdf" label="Plot P-value from sampling against wallenius distribution" name="sample_vs_wallenius_plot" />
44 </outputs>
45 <tests>
46 <test>
47
48 </test>
49 </tests>
50 <help>
51
52 **What it does**
53
54 Detects Gene Ontology and/or other user defined categories which are over/under represented in RNA-seq data.
55
56 Options map closely to the excellent manual_
57
58 .. _manual: https://bioconductor.org/packages/release/bioc/vignettes/goseq/inst/doc/goseq.pdf
59
60
61 </help>
62 <citations>
63 <citation type="doi">10.1186/gb-2010-11-2-r14</citation>
64 </citations>
65 </tool>